Introduction There is increasing concern levels of vaccination are falling below requirements for herd immunity, potentially placing newborn infants at risk. Most research on attitudes towards infant vaccination has involved mothers with limited research into paternal attitudes. Aim Our hypothesis was that fathers with higher levels of education would display a more positive attitude towards vaccination. Methods Observational study of 400 fathers enrolled within the larger Australian Father’s Study. Institutional ethics approval and consent were obtained via mothers. Fathers were recruited from antenatal clinics and community settings. Researchers administered demographic and attitudinal surveys. Quantitative and qualitative data were collected. ACTR: The primary trial was registered at the Australasian Clinical Trials Registry with the number ACTRN Results The majority of fathers had a positive attitude towards infant vaccination (N=357, 89%). However, 9% (N=35) of fathers had a neutral attitude and 2% (N=8) reported a negative attitude. The demographics of the population were similar in education, employment and smoking status to the wider Australian population of fathers Fathers with a negative attitude to vaccination self-reported a higher level of knowledge of pregnancy related issues, yet were significant less likely to have obtained information from a healthcare professional and were instead more likely to have relied upon the Internet as a source of information (both p<0.05) (Table 2). Conclusions The findings suggest that the majority of expectant fathers have a positive attitude toward childhood immunisation. Actual level of paternal education did not impact upon vaccination attitude. However, fathers who self reported a higher knowledge of pregnancy issues and accessed information from the Internet, as opposed to healthcare providers, were more likely to view vaccination negatively. Healthcare staff engaging fathers in vaccination discussions may improve vaccination rates. Natasha Prosser, Rodney Petersen 2, Julie Quinlivan 1,3. 1 Department of Obstetrics and Gynaecology, Joondalup Health Campus; 2 University of Adelaide, Australia; 3 University of Notre Dame Australia, Australia. Literature cited 1.Brown KF, Kroll JS, Hudson MJ, Ramsay M, Green J, Long S, Vincent CA, Fraser G, Sevdalis N. Factors underlying parental decisions about childhood vaccinations including MMR: A systematic review. Vaccine, 2010; (28) Gust D, Brown C, Sheedy K, Hibbs B, Weaver D, Nowak G. Immunization attitudes and beliefs among parents: Beyond a dichotomous perspective. Am J Health Behav, 2005; 29(1): Ramsay ME, Yarwood J, Lewis D, et al. Parental confidence in measles, mumps and rubella vaccine: evidence from vaccine coverage and attitudinal surveys. British Journal of General Practice, 2002; 52, Wu AC, Wisler-Sher DJ, Griswold K, et al. Postpartum Mothers’ attitudes, knowledge and trust regarding vaccination. Matern Child Health J 2008; 12: Australian Father’s Study: What attitudes do expectant fathers have towards infant vaccination? Australian Father’s Study