Drosophila Identification of various phenotypes. Biology 22 Drosophila Identification of various phenotypes.

Outline Brief description of Drosophila phenotypes and life cycle 1-Experiment 1: collection of data related to gender and body color 2-Experiment : 3 point Test Cross How to study keep your flies still during the identification.

Reference for Pictures: Carolina Drosophila melanogster Manual (45-2620) Published: Carolina Biological Supply Company Based on research of Dr. Thomas Hunt Morgan

1866-1945

The life Cycle

Life Cycle: Drosophila melanogaster Eggs: contain filaments 2 molting phases “instars” Life cycle is 10 days

Fruity Fly Egg and Larva

Fruit Fly Pupa and Adult

1- EXPERIMENT: Identification of: Gender Body Color

Wild Type Versus Mutants Body Color Wild type: Beige Mutant: Yellow body

Parental phenotypes Experiment: body color A= female: wt (beige) B=female: wt (beige) C=male: wt (beige) D=female: mutant (yellow) E=male: mutant (yellow)

Fruit Fly Body Color

Sex combs: Male Drosophila

Female and Male Fruit Flies

Abdominal segements 7 segments to female abdominal segments 5 segments to male abdominal segments Male body size is smaller than female But the “last” abdominal segment is larger in male

2- Experiment: 3 point test cross: Eye color Body color Wing length

3 point test cross for: 1. Body Color 2. Wing length 3. Eye Color ♦ Wt vs. Mutant Beige (bl+) vs. Black (bl) Long(vg+) vs. Vestigal(vg) Brick red (br+) vs. brown (br)

Crosses: Female Males Males and female progeny from this F2 cross are your 3 point test cross results! bl+ vg+ Bw+ bl vg bw X bl vg bw bl vg bw

There are all sorts of eyes!

Eyes

You will have only red eyes (wt)

Fruit Fly Wings

Nap Time Triethylamine

How to anesthetize a fly

How to use the Napper

View the Fruit Fly

You will record class data After counting the crosses record the data on the class spreadsheet