Chapter 4, 5, 6
Chapter 4: Microscopy, Staining, and Classification
Units of Measurement Measurement used to measure microbes Metric Units of Length
Microscopy General Principles of Microscopy Light Microscopy Electron Microscopy Probe Microscopy
Staining Preparing Specimens for Staining Principles of Staining Simple Stains Differential Stains Special Stains Staining for Electron Microscopy
Classification and Identification of a Microorganism Linnaeus and Taxonomic Categories Domains Taxonomic and Identifying Characteristics Taxonomic Keys
Chapter 5: Microbial Metabolism
Basic Chemical Reactions Underlying Metabolism Catabolism and Anabolism Oxidation and Reduction Reactions ATP Productions and Energy Storage Roles of Enzymes in Metabolism
Carbohydrate Catabolism Glycolysis Cellular Respiration Alternatives to Glycolysis Fermentation
Other Catabolic Pathways Lipid Catabolism Protein Catabolism
Photosynthesis Chemicals and Structures Light-Dependent Reactions Light-Independent Reactions
Other Anabolic Pathways Carbohydrate Biosynthesis Lipid Biosynthesis Amino Acids Biosynthesis Nucleotides Biosynthesis
Integration and Regulation of Metabolic Functions Control of gene expression Metabolic expression – Synthezing or degrading channel proteins and enzymes – Sequestering reactions in membrane bound organelles – Feedback inhibition
Chapter 6: Microbial Nutrition and Growth
Growth Requirements Nutrients: Chemical and Energy Requirements Physical Requirements Associations and Biofilms
Oxygen Requirements Obligate aerobes Obligate anaerobes
Culturing Microorganisms Clinical Sampling Obtaining Pure Cultures Culture Media Special Culture Techniques Preserving Cultures
Growth of Microbial Populations Mathematical Considerations in Population Growth Generation Time Phases of Microbial Population Growth Continuous Culture in a Chemostat Measuring Microbial Reproduction