/2011/07/02/say-cheese-for-scanning-electron-microscopy/ Life Science Mr. Hooper September 2015

Velcro

Butterfly eggs

Influenza virus particles

Bread mold penny

Salt and PepperPollen grains

Microscopic Images Common House Fly Scanning Electron Microscope (SEM)

Most light microscopes have two glass lenses and are called compound microscopes. The light microscope is an instrument that uses light to produce an enlarged image of the object being examined. Light microscopes can magnify an object up to 1000 times.

Eyepiece: to look through. Magnifies 10x. Body tube: Maintains correct distance between eyepiece lens and objective lens. Coarse adjustment: Moves body tube or stage up and down; large movements. Fine adjustment: moves body tube or stage very small distance; fine focusing. Revolving Nosepiece: Holds objective lenses; revolves. Scanning objective: shortest objective (4x). Low power objective: short objective (10x). High power objective: long objective (40x).

Arm: Supports body tube. Stage: platform that holds specimen slides. Stage clips: Holds slide to stage. Diaphragm: controls amount of light passing through specimen. Mirror/Light Source: illuminates specimen. Base: Supports microscope.

Eyepiece magnificationObjective magnificationTotal magnification 4.10x400x 5.100x500x 6.8x50x What is the total magnification when: 1.Eyepiece is 10x and objective is 10x? 2.Eyepiece is 10x and objective is 50x? 3.Eyepiece is 6x and objective is 100x? Complete this table (4-6)

History of the Microscope

Anton van Leeuwenhoek Leeuwenhoek was a Dutch businessman who sold cloth, and in his spare time built simple microscopes. Leeuwenhoek is credited with discovery of bacteria, protozoa, algae, red blood cells, and spermatozoa. All of these he observed with his simple microscopes.

Anton van Leeuwenhoek Leeuwenhoek studied 67 kinds of insects as well as spiders. He examined life cycles from egg to larva to adult. Examined various types of tissues including muscle, bone, and nerve. First to observe RBCs, bacteria, protozoa, spermatozoa, nematodes, rotifers. October 24, 1632

Sends electrons over the surface of a specimen. Produces a three- dimensional image. SEMs can magnify a specimen up to 150,000 times.

Cellar spider

Send electrons through a very thinly sliced specimen. TEMs can magnify a specimen up to 500,000 times.

Bacillus subtilis - bacteriaplant cell

Does not harm living specimens, like electron microscopes. Uses sound waves to “see” an object. Can be used to examine living cells and for cancer detection.

Ballistic Forensics Investigators can examine the striations on bullets to determine if they were fired from a particular gun. CSI – Crime Scene Investigation Trace evidence such as hair and fibers can be examined microscopically.

leukemia African Sleeping Sickness parasites

Microscopic stream analysis of the number and diversity of living things in a particular area over time.

HIV (human immunodeficiency virus) particles, colored transmission electron micrograph (TEM). Each particle consists of an RNA (ribonucleic acid, purple) genome, surrounded by a protein nucleocapsid (coat, yellow). This is enclosed in a matrix of proteins (grey) and the whole virus is enclosed in an envelope (purple).

Preparing a Wet Mount Slide See Appendix B Pg. 197 of Cells and Heredity

Anton van Leeuwenhoek. Bacillus subtilis.TEM. bacillus-subtilis-bacteria-tem. bacillus-subtilis-bacteria-tem Balloons. Bullet with striations. Chronic Lymphycytic leukemia. Common House Fly. Hair. Healthy Blood Smear. Influenza virus Leeuwenhoek microscope. Leeuwenhoek portrait. Jan%20Verkolje jpg. Jan%20Verkolje jpg Rotifer. Penicillium sp. Pollen. 3cwHBJMhY3A/UWYwIYFaVZI/AAAAAAAAH7c/k9fh78P13I8/s1600/pollen.jpg Salt and Pepper SEM. SAM of bone. SAM Set Up. -acoustic-microscope.html T.Brucei blood smear. TEM. Plant cell.