1 SURVEY OF BIOCHEMISTRY Course Review

2 PRS-1 Which amino acid contains a ring system? 1.Phe 2.Ile 3.Val 4.Gly

3 PRS-1 Which amino acid contains a ring system? 1.Phe 2.Ile 3.Val 4.Gly

4 PRS-2 Which enzyme is an oxidoreductase? 1.Hemoglobin 2.Enolase 3.GAP Dehydrogenase 4.Aconitase

5 PRS-2 Which enzyme is an oxidoreductase? 1.Hemoglobin 2.Enolase 3.GAP Dehydrogenase 4.Aconitase What gets oxidized? What gets reduced?

6 PRS-3 In mixed inhibition ___________. 1.V max increases and K M increases 2.V max decreases and K M increases 3.V max stays the same and K M increases 4.V max decreases and K M stays the same

7 PRS-3 In mixed inhibition ___________. 1.V max increases and K M increases 2.V max decreases and K M increases 3.V max stays the same and K M increases 4.V max decreases and K M stays the same

8 PRS-4 A fatty acid with 18 carbons and no double bonds in the alkyl chain is called: 1.Stearic acid 2.Palmitic Acid 3.Linoleic acid 4.Arachidonic acid

9 PRS-4 A fatty acid with 18 carbons and no double bonds in the alkyl chain is called: 1.Stearic acid 2.Palmitic Acid 3.Linoleic acid 4.Arachidonic acid

10 PRS-5 An alpha helix is stabilized by: 1.Hydrogen bonds between the side chains of the amino acids 2.Hydrogen bonds between the carbonyl oxygens and the N-H group of the adjacent residue 3.H-bonds between the carbonyl oxygens and the N-H group of the (n+3)th residue 4.H-bonds between the carbonyl oxygens and the N-H group of the (n+4)th residue

11 PRS-5 An alpha helix is stabilized by: 1.Hydrogen bonds between the side chains of the amino acids 2.Hydrogen bonds between the carbonyl oxygens and the N-H group of the adjacent residue 3.H-bonds between the carbonyl oxygens and the N-H group of the (n+3)th residue 4.H-bonds between the carbonyl oxygens and the N-H group of the (n+4)th residue

12 PRS-6 In ion exchange chromatography, the protein captured by the resin is eluted with: 1.Salt 2.Water 3.Imidazole 4.Diethylaminoethyl groups

13 PRS-6 In ion exchange chromatography, the protein captured by the resin is eluted with: 1.Salt 2.Water 3.Imidazole 4.Diethylaminoethyl groups

14 PRS-7 What reaction follows GAP dehydrogenase in glycolysis? 1.Phosphoglucomutase 2.Phosphofructokinase 3.Phosphoglycerate kinase 4.Phosphoglycerate mutase

15 PRS-7 What reaction follows GAP dehydrogenase in glycolysis? 1.Phosphoglucomutase 2.Phosphofructokinase 3.Phosphoglycerate kinase 4.Phosphoglycerate mutase

16 PRS-8 What is Dansyl Chloride used for? 1.End group analysis in peptide sequencing 2.Staining polyacrylamide gels 3.Determining protein concentration 4.Reducing disulfide bonds

17 PRS-8 What is Dansyl Chloride used for? 1.End group analysis in peptide sequencing 2.Staining polyacrylamide gels 3.Determining protein concentration 4.Reducing disulfide bonds

18 PRS-9 Which amino acids make up the catalytic triad of serine proteases? 1.His, Lys, Arg 2.Ser, His, Asp 3.Asp, Cys, Ser 4.Lys, Ser, Asp

19 PRS-9 Which amino acids make up the catalytic triad of serine proteases? 1.His, Lys, Arg 2.Ser, His, Asp 3.Asp, Cys, Ser 4.Lys, Ser, Asp

20 PRS-10 Which of the following is NOT a disaccharide? 1.Lactose 2.Maltose 3.Sucrose 4.Mannose

21 PRS-10 Which of the following is NOT a disaccharide? 1.Lactose 2.Maltose 3.Sucrose 4.Mannose

22 PRS-11 How many fused rings are found in cholesterol? 1.One 2.Two 3.Three 4.Four

23 PRS-11 How many fused rings are found in cholesterol? 1.One 2.Two 3.Three 4.Four

24 PRS-12 Which of the following cannot serve as a nucleophile? 1.Ser 2.His 3.Asp 4.Zn 2+

25 PRS-12 Which of the following cannot serve as a nucleophile? 1.Ser 2.His 3.Asp 4.Zn 2+