gRAD (generic Rapid Assay Device) New Product Introduction Dina Gonçalves December 2015

What, How and Why? What is gRAD? How does gRAD works? Why to use gRAD? Products portfolio Where and how to sell gRAD Customers References Market strategies Competing technologies/approaches Unique selling points Marketing Material Introductory special offer to distributors Outline

What, How and Why?

What is gRAD? sample pad sample pad conjugate pad test line biotin-binding protein test line specific antibodies control line anti-mouse / rabbit / goat antibodies control line generic antibodies Standard lateral flow (test-specific) gRAD (generic)

What is gRAD? flow Standard lateral flow (test-specific)

What is gRAD? Standard lateral flow (test-specific) Assay-specific Defined antibodies in the strip (detection and conjugated to gold) Biological recognition events on strip By custom outsourced development or requiring especial printing equipment

How does gRAD works? sample pad test line biotin-binding protein control line anti-mouse / rabbit / goat antibodies 1 test line + 1 control line Serum, plasma, urine, solutions of antigen, solid material dissolved in diluent Consistency of test line – CV 2-5% 50 pg/ml – 500 ng/ml, on device Monoclonal antibodies, polyclonal affinity-purified, provided by the user Mouse, rabbit or goat antibodies gRAD (generic)

flow How does gRAD works? B B B detection antibody conjugated to gold antigen-antibodies complex biotinylated capture antibody antigen gRAD (generic)

How does gRAD works? B B B gRAD (generic)

How does gRAD works? Developing and optimizing an assay a.Producing gold conjugates b.Finding the analyte concentrations giving a signal (visible test line) c.Optimization of the capture reagent d.Optimization of the detection reagent Running an optimized assay

How does gRAD works? Important points to consider Gold conjugation Testing different capture antibody concentrations Low antibody concentration  Higher sensitivity, but slower and less precise assay High antibody concentration  Lower signal intensity, but faster and more precise assay Testing different gold conjugated antibody volumes Increasing volume detection Ab  Test line signal increases, but also the background

How does gRAD works? Developing and optimizing an assay Running an optimized assay a.Pre-test user steps i.Conjugation ii.Mixing components b.Test user steps i.Dipping strip in reaction mix ii.Evaluation of test line intensity

How does gRAD works? PRE-TEST USER STEPS TEST USER STEPS Test Reagent Test Mixture

Why to use gRAD? Easier and faster in-house development of rapid assays Multiple uses and applications Simple procedures No need for specialized equipment Development of niche applications Possible quantification without need of a readers and expensive software Keeping internal knowledge Different levels of complexity

REFPRODUCT LIST PRICE gRAD(1) - KIT gRAD OneDetection - Kit 900 € gRAD(1) gRAD OneDetection – 120 strips 480 € NGIB18-1 Naked Gold Conjugation Kit (20 nm & 40 nm) 480 € NGIB18-2 Naked Gold Conjugation Kit (20 nm) 480 € NGIB18-3 Naked Gold Conjugation Kit (40 nm) 480 € SDB50 Sample Dilution Buffer 10 € The complete solution to start developing gRAD assays Perfect for testing new ideas and running optimized assays Optimized gold conjugation kit Optimized buffer to enhance gRAD assays performance Products Portfolio

gRAD(1) - KITgRAD(1) SDB50 NGIB18 Products Portfolio calibration card

Where and how to sell?

Customers Research and Development in: Veterinarian applications Livestock Companion animals Feed contamination control Milk and meet quality control Diagnostics, human Tropical diseases Cancer Biomarkers pre-screening Quality control, pharma and biologics …and more…

References Foot and mouth disease Yang et al. (2015) Development of a multiplex lateral flow strip test for foot-and-mouth disease virus detection using monoclonal antibodies. J. Virol. Methods 221: Yang et al. (2013) Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic Rapid Assay Device. Virol. J. 10:125.

Competing technologies/approaches Outsourcing custom development Development in-house from scratch Other rapid assays technologies

Marketing strategies Materials Mailings Video Printed material Campaigns Entry offer / trial - 20 strips for free Partner Program (for high-impact academic institutions) Demos / Conferences 2016 Custom service / offers Discount matrix for bulk orders

Marketing material Website Video Printed Material

Reactions in solution Open and Flexible Platform Qualitative Semi-Quantitative Possible Quantitative Assays Patented Technology (WO A1) Total customization Easier and faster Time and Money Saving Ready-to-use kit format Unique selling points

Dec 2015 ORDER in December 2015 Get EXTRA DISCOUNT on gRAD products purchase 2016 Total purchase target value in 2016 % purchase value in gRAD products 2017 Guaranteed extra discount of 5% on gRAD products (3 distributors awarded) Introductory special offer to distributors

Any purchase (min 1 x gRAD(1) – KIT + other product(s) at least the same value) Extra* 10 % on gRAD 5 x gRAD(1) – KIT or 3 x gRAD(1) – KIT + 4 gRAD(1) – 120 or 2 x gRAD(1) – KIT + 6 gRAD(1) – 120 Extra* 20 % on gRAD >10 x gRAD(1) – KIT or 5 x gRAD(1) – KIT + 10 gRAD(1) – 120 Extra* 30 % on gRAD * On top of distributor discount (the same you have for Abs/ELISAs) Extra discount December 2015

GROUP MINIMUM PURCHASE TARGET Group A (small distributors)5.000 € Group B (medium distributors) € Group C (large distributors) € Qualification for extra discount 2017

Make all your questions! Return feedback from customers Keep close contact to provide excellent support Share special requests from customers, ideas for improvement and new gRAD versions Share feedback on interest for quantitative setup Co-market gRAD with other products (antibodies, matched pairs, etc..) Share your ideas to reach new customers Making gRAD a success together!

BioPorto Diagnostics A/S Tuborg Havnevej 15, st. DK-2900 Hellerup Denmark Phone(+45) Webwww.bioporto.comwww.bioporto.com LinkedInhttps:// Thank you. Dina Goncalves, PhD Key Account Manager