Laboratory microwave oven 2.45 GHz multimode microwave reactor Maximum power of 900w The experimental SFME variables were optimised in order to maximise the essential oil yield Atmospheric pressure. 150g fresh plant material Solvent or water X Power of 150w heated Essenntial oil and aromatic water was simply seperated by decantation.

The MAC-75 apparatus is multimode microwave reactor. Microwave cavity – 150L, PTEE drum – 75L Cavity 6 external tube connection The system automatically adjusts the power delivered if absorption is too low.

Essential oil Dried under anhydrous sodium sulphate

Agilent 6850 gas chromatograph Vector gas : Helium Detector temperature : 250 ℃ Injected volume 1 Split ration 1/100 Hp1 column : polydimethylsiloxane (10m X mm i.d., film thickness X 0.4m constant flow 0.3mL/min) Three replicates were performed for each sample The average of these three values and the standard deviation were determined for each component identified.

Agilent 5973 MS Carrier gas : helium Constant flow 1mL min-1 Injector temperature ℃ or 230 ℃ at 2 /min then held isothermal(20min)at 250 ℃ or 230 ℃ Transfer line temperature 250 ℃ (aplolar column) or 230 ℃ (polar column) Ionisation energy 70eV mass range amu Fused-silica capillary column HP-IMS TM (polydimethylsiloxane, 50m X 0.25 mm i.d. X film thickness 0.25 ℳ m ) INNOWAX (polyethyleneglycol, 50m X 0.20 min i.d. X film thickness 0.4mm)

Spectra organized by positive/negative charge, mass, compound name, and conditions. Combined with a comparison of GC retention indices(R1) on a polar and polar columns. RIs were calculated with the help of a series of linear alkanes C6-C27 on apolar and polar columns (HP-1MS TM and HP-INNOMAX) Compounds available in the laboratory were confirmed by external standard com pound co-injection