Early neutralizing IgG response to Chikungunya virus in infected patients targets a dominant linear epitope on the E2 glycoprotein by Yiu ‐ Wing Kam, Fok.

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Presentation transcript:

Early neutralizing IgG response to Chikungunya virus in infected patients targets a dominant linear epitope on the E2 glycoprotein by Yiu ‐ Wing Kam, Fok ‐ Moon Lum, Teck ‐ Hui Teo, Wendy W. L. Lee, Diane Simarmata, Sumitro Harjanto, Chong ‐ Long Chua, Yoke ‐ Fun Chan, Jin ‐ Kiat Wee, Angela Chow, Raymond T. P. Lin, Yee ‐ Sin Leo, Roger Le Grand, I ‐ Ching Sam, Joo ‐ Chuan Tong, Pierre Roques, Karl ‐ Heinz Wiesmüller, Laurent Rénia, Olaf Rötzschke, and Lisa F. P. Ng EMBO Mol Med. Volume 4(4): April 4, 2012 ©2012 by European Molecular Biology Organization

Antigenic recognition by CHIKV ‐ infected patients' samples. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization

Epitope mapping of the E2 glycoprotein. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization

Localization of the E2EP3 epitope. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization

Alanine ‐ scan analysis of E2EP3 by anti ‐ CHIKV antibodies. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization

Depletion of anti ‐ E2EP3 antibodies reduces neutralizing activity against CHIKV infection. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization

Sero ‐ positivity of anti ‐ E2EP3 IgG3 antibodies in CHIKV ‐ infected patients from other cohorts. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization

IgG from CHIKV ‐ infected NHP plasma recognize E2EP3 and neutralize CHIKV infection in ‐ vitro. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization

Protection against CHIKV challenge in mice vaccinated with E2EP3 peptide. Yiu ‐ Wing Kam et al. EMBO Mol Med. 2012;4: ©2012 by European Molecular Biology Organization