LABORATORY OF RADIATION BIOLOGY Radiaton Induced Apoptosis in Human Lymphocytes Joint Institute for Nuclear Research Karolina Ficenzova, Martin Sefl Faculty.

Slides:

Advertisements

Similar presentations

Radiation Safety Course: Biological Effects

Advertisements

The Radiobiology of Radiation Therapy. Type of Injuries Nuclear DNA is major target Nuclear DNA is major target Cellular membrane damage – minor Cellular.

Computer modeling of radiation effects Noriyuki B. Ouchi and Kimiaki Saito Radiation Effects Analysis Research Group, Nuclear Science and Engineering Directorate,

Andrew J. Pierce TOX 780 Single and Double-strand Break Repair TOX 780 Andrew Pierce Microbiology, Immunology and Molecular Genetics Toxicology University.

Module 4 Biological Effects of Ionizing Radiation

Mathematical Modeling of Physio-chemical Phase of the Radiobiological process J. Barilla, J. Felcman, S. Kucková Department of Numerical Mathematics, Charles.

DNA damage, repair and recombination

Alpha-particle-induced bystander effects between zebrafish embryos in vivo E.H.W. Yum 1, V.W.Y. Choi 1, V.W.T. Li 2, S.H. Cheng 2, and K.N. Yu 1 1 Department.

THE INFLUENCE OF THE P53STATUS FOR BIOLOGICAL EFFECTS OF THE GLIOBLASTMA CELLS FOLLOWING BORON NEUTRON CAPTURE THERAPY Keiko Seki, Yuko Kinashi, Sentaro.

May 01RWS Development of Nanodosimetry for Biomedical Applications Project Goals and Current Status.

What is apoptosis?? Apoptosis is a morphological term

Bystander Effects.

Characterization of a potential new drug in cancer therapy Lab 2 Salah Farag.

BIOLOGICAL EFFECTS OF IONIZING RADIATION AT MOLECULES AND CELLS.

Studying of regularity of programmed cell death after influence of demages factors Speaker: Karolina Socha 2011 Student Practice in JINR Fields of Research.

TRAINING COURSE ON RADIATION DOSIMETRY: Radiobiology Basics – RBE, OER, LET Anthony WAKER University of Ontario Institute of Technology Thu. 22/11/2012,

Cell Injury Cell and Tissue Adaptation Necrosis and Apoptosis Dr. Raid Jastania.

Fig. S1. Modulation of chromatin structure of AS52 cells by increasing concentrations of resveratrol, probed by limited digestion with Dnase I: densitometric.

Module 11: Radioecology in the North The three types of radiation Identify radiation as a normal part of the environment Define the concept of half-life.

Chemical-Induced Mutagenesis

General Pathology – Seminar 2 Necrosis Atrophy - causes Jaroslava Dušková Inst. Pathol.,1st Med. Faculty, Charles Univ. Prague

Nermeen Kamel Abe El Moniem Laboratory of Radiation Biology, JINR Supervised by Dr. Oleg Belov Modeling of DNA damages induction under ionizing radiation.

1 IONIZING RADIATION. 2 Non-Ionizing Radiation Does not have enough energy to remove electrons from surrounding atoms.

Introduction to Radioisotopes: Measurements and Biological Effects

Chapter 10: Chemical-Induced Mutagenesis. DNA and Mutations A mutation is a permanent change in the DNA. DNA is in our chromosomes and it codes for all.

AP Biology DNA Replication AP Biology Double helix structure of DNA “It has not escaped our notice that the specific pairing we have postulated.

Molecular methods of cell culture III. Apoptosis  Programmed cell death  A physiological mechanism to eliminate excess, damaged or dangerous cells from.

Mathematical modeling of recombination repair mechanism for double strand DNA breaks in Escherichia coli bacterial cells Alaa Mohamed Researcher Assistant,

2010 Student Practice in JINR Fields of Research

DNA Damage and Biodosimetry

24th International Conference on Nuclear Tracks in Solids Bologna, 1-5 September 2008 Study of influence of catechins on bystander responses in alpha-

Radiation Effects on DNA and Chromosomes. So, what do you understand by DNA anyway? DNA can be described as a long fiber that resembles a hair under a.

Use of Ethidium Bromide in gel electrophoresis  Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in.

MUTAGENS AND MUTAGENESIS. Mutagens  Naturally occurring mutations are referred as spontaneous mutations and are thought to arise through chance errors.

Basic Biologic Interactions of Radiation IONIZATION.

GENETICS ESSENTIALS Concepts and Connections SECOND EDITION GENETICS ESSENTIALS Concepts and Connections SECOND EDITION Benjamin A. Pierce © 2013 W. H.

Gihan E-H Gawish, MSc, PhD Ass. Professor Molecular Genetics and Clinical Biochemistry KSU 10 TH WEEK DNA damage, repair & Mutagenesis.

Methoxyamine (MX) potentiates IUdR-induced radiosensitization by enhancing senescence in RKO cells BACK TO THE BEGINNING.

Fourth lecture.

Fifth Lecture Oxidative damage by free radicals Oxidative damage by free radicals DNA Protein Lipids Cross- linkage Oxidized bases Strand breaks Hydroxylation.

1 Radiobiology & Radiobiology of Accelerated Heavy Ions Haitham A. Shaban Haitham A. Shaban Assistant Researcher, NRC Teaching Assistant, American University.

Programmed cell death is a normal physiological form of cell death that plays a key role both in the maintenance of adult tissues and in embryonic development.

Dr. Mohammed Alnafea Radiation Interaction with matter.

Targeting of reactive oxygen species can be a potential therapeutic strategy for cancer treatment Ying-Ray Lee 1, San-Yuan Chen 2, and Hau-Ren Chen 3 1.

Nature of radioactivity: Spontaneous disintegration of atomic nuclei, usually in nuclei that deviate from a balance of protons & neutrons. Radiation involves.

Comparison of neutron production on lead-uranium setup irradiated by protons and deuterons with different energies Ondřej Svoboda Nuclear Physics Institute,

Lecture 18 DNA Repair Rohini. K. Learning Objective 08 Nov 2011Rohini K FoM 2  An overview of the types of DNA damage  DNA repair mechanisms  Defects.

 When radiation interacts with target atoms, energy is deposited, resulting in ionization or excitation.  The absorption of energy from ionizing radiation.

Rad T 110 Sherer Biologic Effects of Radiation Exposure.

General conclusion on the Laboratory of Radiation Biology poster session Programme Advisory Committee for Condensed Matter Physics 32 nd meeting,

INDIAN INSTITUTE OF TECHNOLOGY ROORKEE Development of 5- fluorouracil loaded hydrogel and study of its anticancer potential Sonal Omer Dr. Bharat Bhushan,

Elucidate the mechanism of anticancer activity of Piper nigrum extract on colon cancer cell line Akila Prashant, MVSST Subbarao, Devananda D, Prashant.

Laboratory of Radiation Biology

Scheme 1 Cu(Sal-Gly)(phen) Cu(Sal-Gly)(pheamine) Cu(Sal-Gly)(phepoxy)

DNA repair kinetics: the effect of dose and radiation quality

Bystander Effects.

Piotr Knapp Piotr Wróblewski

Mechanism of Cell Injury

Tumor Promoting Inflammation

Laboratory of Information Technology

Figure 5 The biological effects of charged particles

Do protons and X-rays induce cell-killing in human peripheral blood lymphocytes by different mechanisms? J. Miszczyk, K. Rawojć, A. Panek, A. Borkowska,

Radioactivity Henri Becquerel discovered X-rays in As a result of his experiments, he also discovered other forms of rays that could be emitted.

LMD-assisted single cell DNA typing of forensic biological evidence: Issues of the cell type and sample condition Sergey Leonov, Elena Zemskova, Pavel.

General Pathology – Seminar 2

T-cell activation and induction of apoptosis in epithelial cells of oral lesions induced by 4-NQO. T-cell activation and induction of apoptosis in epithelial.

Repair kinetics of radiation-induced DNA damage under microgravity conditions. Repair kinetics of radiation-induced DNA damage under microgravity conditions.

Presentation transcript:

LABORATORY OF RADIATION BIOLOGY Radiaton Induced Apoptosis in Human Lymphocytes Joint Institute for Nuclear Research Karolina Ficenzova, Martin Sefl Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague JINR Summer Practice 2010, Dubna

Damage of DNA direct indirect – high energy particle lyse the water and free radicals results (H 3 0 +, OH - ·, HO 2 · )

DNA damages Single-strand break Double strand break Base damage Sugar damage Laboratory of Radiation Biology, JINR

Apoptosis (programmed cell death)  Apoptosis is an important mechanism which provides the removal of weakened, unnecessary, and damaged cells.  Apoptosis is necessary for maintaining the stability of genetic information.  The loss of the spontaneous apoptosis capability results in carcinogenesis.  Apoptosis efficiency is strongly decreased or completely suppressed in cancer cells. Laboratory of Radiation Biology, JINR

6 Donors blood Incubations of the cells for 24, 48, 72 h Lymphocytes separation Irradiation Phasotron Rocus-M detecting with a fluorescent microscope preparations Laboratory of Radiation Biology, JINR

Imaging different forms of cell death Living cells and death cells (thanks to apoptosis or necrosis) were detected with a fluorescent microscope by tracing morphological changes in preparations stained with a mix of dyes – ethidium bromide and acridine orange. necrosisliving apoptosis Laboratory of Radiation Biology, JINR

Apoptotic cell yield 0, 24 and 48 h after irradiation  -rays Protons Laboratory of Radiation Biology, JINR

Influence of DNA repairinhibitors on the induction of DNA lesions Influence of DNA repair inhibitors on the induction of DNA lesions AraC+Hu S 1 endonuclease Double-strand break After γ -irradiation, some difficult-to- repair single-strand DNA breaks (SSB) formed in cells. When Ara-C and Hu were applied, these lesions – unrepaired for a long time – were attacked by S1-endonuclease, which attacked the opposite SSB site turning them into DNA double- strand breaks. As a result, the post- period apoptotic cell output increased. Laboratory of Radiation Biology, JINR

Apoptotic cell output after γ-irradiation with DNA repair inhibitors Ara-C and Hu Laboratory of Radiation Biology, JINR

Influence of DNA repair inhibitors Ara-C and Hu on proton-induced apoptosis Laboratory of Radiation Biology, JINR

Thank you for attention! Laboratory of Radiation Biology, JINR