Procedure for making a wet mount

Slides:

Advertisements

Similar presentations

1 Making and staining a wet mount Refer to the Practical Manual Introduction Sections F.1 and F.2.

Advertisements

Cheek to Cheek Lab Animal Cells Making a Wet Mount Slide 1.Use dropper to place a drop of water on center of slide. 2.Take a toothpick and carefully.

EXTRA CREDIT!!! MAKE YOUR OWN FLASH CARDS BASED UPON WHAT YOU HAVE LEARNED ABOUT CELLS animal vs. plant cells cell structures & functions Minimum of 12.

The Glue Method. 1. Take a clean slide and carefully place a coverslip at one end of it.

Parts of the Compound Light Microscope

VIEWING CELLS: USING THE COMPOUND LIGHT MICROSCOPE & STAINS

PLEASE DO NOT TOUCH THE MICROSCOPES AT YOUR DESKS!!!

Parts of the Microscope and Their Function On the next slide there is an image of a microscope, very similar to the scopes we use in class. Click on the.

DO NOW: 1.Hand in the field trip brainstorming you wrote on Friday. 2.Take out your microscope Green packets from last week. 3.In your NOTES section of.

DO NOW: Take out your microscope YELLOW packets from last week.

Topic: Microscope Aim: How do we use the compound microscope? Do Now: Take out your homework. Identify the microscope part being described as I walk around.

Microscope Lab. Mrs. Orwar’s sure fire way to find an image every time! 1.Start on low power (red objective) 2.Place your slide centered over the light.

 A form of energy that travels in waves  These waves are a form of electromagnetic radiation. Light is not the only form of electromagnetic radiation.

Preparing a slide Before adding the sample, clean the slide with ethanol and cotton wool. Hold at a 45° angle Lower slowly to avoid forming air bubbles.

The Compound Microscope

Anton van Leeuwenhoek. Anton van Leeuwenhoek (1600’S) -created 1 st microscope. He noticed that if he placed 2 magnifying lenses at proper distances from.

T. Trimpe Body Tube Nosepiece Objectives Stage Clips Light Ocular lens (Eyepiece) Arm Stage Coarse Adjustment Fine Adjustment.

Introduction to the Microscope  Care  Parts  Focusing  Wet mounts  Calculating magnification.

Microscopes Biology Light Microscope (LM) [aka Compound Microscope] Visible light is projected through the specimen. Glass lenses enlarge the image &

Types of Microscopes 0.1 mm is the smallest object that is seen with the human eye. When we view with a microscope, we are either viewing in micrometer.

Lab 1: Using the Microscope

Intro to the Microscope. Parts of a Microscope Objective Lens: Lens that receives the first rays of light from the light source. 3 magnifications:

 A form of energy that travels in waves  These waves are a form of electromagnetic radiation. Light is not the only form of electromagnetic radiation.

Light Microscopes. Light Microscopes allow scientists to see objects up to 400x their natural size. Scientists view objects that are on glass slides.

Chapter 7 Osmosis lab. Materials red onion forceps metric ruler Scissors paper towel iodine solution microscope slide coverslip dissecting probe microscope.

HOW TO USE THE MICROSCOPE

Microscope Notes. Parts of a microscope 1. Eye piece/ocular- the piece that is looked through, has a power of Body tube- skinny tube attached to.

Microscopes. Handling & Care Carrying the Microscope –Carry from the base & arm, use 2 hands! Using the Microscope –Always use tissue paper to clean lenses!

Introduction to Microscopes A lesson in the care and use of compound microscopes.

EXTRA CREDIT!!! MAKE YOUR OWN FLASH CARDS BASED UPON WHAT YOU HAVE LEARNED ABOUT CELLS IN THIS UNIT animal vs. plant cells cell structures & functions.

Microscopy Techniques and Calculations. Types of Microscopes.

Bell Ringer: 1. What are cell membranes made of? 2. What happens to a cell if it is placed in salt water (hypertonic solution)? 3. What is the function.

Do Now(1) How many: Cm in 4 meters? Decimeters in 5 meters?

Microscope and Other Tools

Preparing Wet Mount Slides

Topic: Microscope Aim: How do we use the compound microscope? Do Now:

Introduction to the Microscope  Parts  Care  Focusing.

Prepared By: Ms. Carolyn R. Magat

GREEN BOOK REVIEWS – STATE LAB

An introduction to the Compound Light Microscope (This is a note check, for p. 3) You must make sure you have these correct for studying them later.

TOPIC: Microscope AIM: How do we use a microscope?

Unit 2: Cells and Cell Structure

Using the Microscope.

Important Microscope Techniques

Using the Microscope for the Cheek &

The Compound Microscope

Parts of the Compound Light Microscope

Microscope Basics T. Trimpe

What is the function of the vacuole?

Types of Microscopes 0.1 mm is the smallest object that is seen with the human eye. When we view with a microscope, we are either viewing in micrometer.

Microscope Review.

Microscope Use.

Microscopes!!!.

AIM: HOW DO WE USE MICROSCOPES?

Do Now(1) How many: Cm in 4 meters? Decimeters in 5 meters?

Microscope Calculations

Teaching a Lesson LE Lesson Using Regents Diagrams

Light Microscope: – light passes through one or more lenses to produce an enlarged image of a specimen compound light microscope – light microscope that.

Microscope Intro Honors Bio.

Light Microscope: – light passes through one or more lenses to produce an enlarged image of a specimen compound light microscope – light microscope that.

Microscope Review.

Microscopes Always use 2 hands to carry; one on the arm and one on the base Plug in; turn light on.

Microscope Intro Honors Bio.

Microscopy information

Locard’s Exchange Principle

Microscope Identification

Eyepiece/Ocular Coarse Adjustment Fine Adjustment Arm Objective

Light Microscope: – light passes through one or more lenses to produce an enlarged image of a specimen compound light microscope – light microscope that.

Presentation transcript:

Procedure for making a wet mount

Procedure for making a wet mount Step One: Use the lens paper to clean a glass slide and coverslip. Step Two: Place the specimen that you wish to observe in the center of the slide. Step Three: Using a medicine dropper, place one drop of water on the specimen. Step Four: Position the coverslip so that it is at a 45 degree angle to the slide *Make sure that the water runs along the edge of the coverslip Step Five: Lower the coverslip slowly to avoid trapping air bubbles.

Procedure for making a wet mount cont. Step Six: If a stain solution will be added to a wet mount, place a drop of the staining solution to the microscope slide along one side of the coverslip. Place a small piece of paper towel on the opposite side of the coverslip. Step Seven: As the water evaporates from the slide, add another drop of water by placing the tip of the medicine dropper next to the edge of the coverslip, just as you would if adding stains or solutions to a wet mount. If you have added too much water, remove the excess by using the corner of a paper towel as a blotter. Do not lift the coverslip to add or remove water.