Investigating Root Tip Mitosis

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Presentation transcript:

Investigating Root Tip Mitosis

Learning outcomes You will prepare temporary slides of cells found in garlic root tip meristem tissue use a light microscope to observe, identify and draw root tip meristem cells in different stages of the cell cycle measure the sizes of some of these cells calculate the mitotic index for the garlic root tip tissue that you observe

Safety 1M Hydrochloric acid is an irritant. Wear eye protection and rinse off any splashes or spills immediately with cold water Toluidine blue is harmful if ingested and will stain skin and clothes. Wear eye protection and protective gloves and rinse off any splashes or spills immediately with cold water Take care when using sharp instruments such as scalpels, scissors and mounted needles and follow the instructions for their safe use that have been given to you by your teacher.

Use a 3-day old rooted garlic clove, suspended over water with a cocktail stick.

Leave the beaker containing the garlic clove in acid in the water bath at 40oC for 5 minutes.

Use the cocktail stick to carefully remove the garlic clove from the acid and gently rinse the roots in tap water.

Using scissors, cut off the terminal 3mm of the root tips and allow them to fall onto a small watch glass.

Leave the garlic in the stain at room temperature for 2 minutes Leave the garlic in the stain at room temperature for 2 minutes. Remove the excess stain and rinse the root tips in the watch glass with tap water.

Cover the slide and coverslip with a paper towel by wrapping the towel around the slide several times. Gently press down on the coverslip with a thumb to squash the root tips and to spread them out.

Spend a few minutes looking over the whole of your prepared slide Spend a few minutes looking over the whole of your prepared slide. You are looking for small, square cells that are found in rows.

Calculate the mitotic index for your chosen field of view, using the formula MITOTIC INDEX = NUMBER OF CELLS IN THE FIELD OF VIEW UNDERGOING CELL DIVISION TOTAL NUMBER OF CELLS IN THE FIELD OF VIEW

MITOTIC INDEX = NUMBER OF CELLS IN THE FIELD OF VIEW UNDERGOING CELL DIVISION TOTAL NUMBER OF CELLS IN THE FIELD OF VIEW

This image may be used as sample data to calculate the mitotic index for garlic root tip tissue.