Primers to map bsd deletion points on genomic DNA NameForwardReverse Amplicon size (base pairs) A15’-CCACGGATGGAGTGAGTTCT-3’5’-GCCCCCAAGATGAGGATTAT-3’931.

Slides:



Advertisements
Similar presentations
RAPD Randomly Amplified Polymorphic DNA
Advertisements

Original Tree:
Figure 1. Melting curve of SNP marker D1 of probe and PCR amplicon on LightScanner. SNA D1 is CA or CA deletion. It is very clear to see the genotype by.
1 times table 2 times table 3 times table 4 times table 5 times table
University of Oklahoma Genome Center4/14/12.
4-3 Relations Objective: Students will represent relations as sets of ordered pairs, tables, mappings, and graphs. Students will find the inverse of a.
Genomic walking (1) To start, you need: -the DNA sequence of a small region of the chromosome -An adaptor: a small piece of DNA, nucleotides long.
Identification of an AFLP marker for stem rust resistance in Avena strigosa Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari AAFC-CRC, Winnipeg.
DNA Bases. Adenine: Adenine: (A) pairs with Thymine (T) only.
SV validation plate #1 Format: 384 amplicons ( two 384-well plates of primers ) Events: 4 different types of SVs: Deletions Insertions Tandem duplications.
File 5 MOUSE sequences used in target gene promoter regions to detect NFATc1 protein binding by ChIP.
Molecular Cloning.
$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.
Tables Learning Support
第 8 章 PowerPoint 2003 的使用 1 PowerPoint 2003 窗口简介 2 PowerPoint 2003 演示文稿的创建与放映 3 幻灯片的基本操作.
Genome evolution within the individual
PCR & visualise products on gel
PCR conditions: 94ºC for 15 min
(A) SGT Antisense RNA construct
Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.
Myopodin, a Synaptopodin Homologue, Is Frequently Deleted in Invasive Prostate Cancers  Fan Lin, Yan-Ping Yu, Jeff Woods, Kathleen Cieply, Bill Gooding,
Pre-genomic era: finding your own clones
Hire Toyota Innova in Delhi for Outstation Tour
Times Tables.
Supplementary Figure 1 A B wild type ΔacsS
Fig. S Fig. S2 Cre-mediated recombination in vivo. G2 mice displaying high levels of GFP were crossed.
PCR genotype analysis to determine RNP-mediated knockout efficiency in C. lusitaniae. PCR genotype analysis to determine RNP-mediated knockout efficiency.
1.6 Describing Pairs of Angles
A C Tumour Chromosome 8 Tumour Chromosome 8 B D Tumour Chromosome 17
A B Tumour 74 – Whole Genome Tumour 74 – Whole Genome C D
إعداد المشرفة التربوية نجلاء الجارد
© 2013 Elsevier, Inc. All rights reserved.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
Relations.
In groups of 3 at your seat, list all you can about this table.
cpDNAs with large deletions accumulate in cptk1 mutants.
2.1: Represent Relations and Functions HW: p.76 (4-20 even, all)
Relations for functions.
Slope  4 Ways: Graph Formula Ordered Pairs Table.
Objectives The student will be able to:
1.6 - Relations.
Lisa Edelmann, Jianli Dong, Robert J. Desnick, Ruth Kornreich 
Ricardo A. Leite, Maria C. Marchetto, Alysson R
Relations and Functions
Development of a SNaPshot® Multiplex system for the typing of single nucleotide polymorphisms (SNPs) involved in the adaptive response to high altitude.
3-1 Relations A relation is a set of points.
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
Proportion of 16S rRNA gene sequences in each category of phylogenetic novelty relative to cultures for each environment, by amplicons, metagenomes (without.
Definition Here Vocabulary Word Here Definition Here
The FRAME Routine Functions
3 times tables.
Objectives The student will be able to:
6 times tables.
Objective- To graph a relationship in a table.
X Y Relation (a set of ordered pairs) x y x y ( , ) x y Mapping
Relation (a set of ordered pairs)
Production of Mapk14 and Mapk7 KO cells.
Gel electrophoresis of measles virus cDNA amplicons (HU2 control measles virus RNA) amplified under optimised reaction conditions. Gel electrophoresis.
PCR amplification of the ORF encoding the cytosolic p36 protein of M
Objectives The student will be able to:
Supplementary Table Primer Sequence Reference Region 1 BS-CTCF-725-F
In situ footprinting of the IGRP promoter: primer set C
In situ footprinting of the IGRP promoter: primer set D
Amplicon sequencing analysis of on-target sites in trβ crispants
KEY CONCEPT Entire genomes are sequenced, studied, and compared.
The TSDR of ATL cells exhibits Treg-like hypomethylated status or CD4+ conventional T cell–like methylated status. The TSDR of ATL cells exhibits Treg-like.
Relative abundance and expression of the 10 most abundant MAGs in the bioreactor at day 96. Relative abundance and expression of the 10 most abundant MAGs.
Mitochondrial DNA as a Cancer Biomarker
Presentation transcript:

Primers to map bsd deletion points on genomic DNA NameForwardReverse Amplicon size (base pairs) A15’-CCACGGATGGAGTGAGTTCT-3’5’-GCCCCCAAGATGAGGATTAT-3’931 A25’-ATGGGCATCGTCTTTTTCAG-3’5’-CCTCTCCCACCCTTACCAGT-3’895 B15’-GATGAGAAAGCCCTTTGCAG-3’5’-CCAGCCAAGAAACTGGACAT-3’873 B25’-GTGTAAGCGCTCCAGGAAAG-3’5’-AGGGCCAATGCATTAAAGTG-3’876 C15’-CCCTGTCCCACTTATGCACT-3’5’-GTCCTTGGCCTTTTTCTTCC-3’956 C25’-GCACACGTTTATGCCATCAC-3’5’-TCCTAGGCAGGCAACAGAAT-3’977 C35’-CCCTCCACTCTTCCTTCCTG-3’5’-CCTTTTTGGGTTGACAATGG-3’953 C45’-CCATGCCAGAGCCTATATTTC-3’5’-AGAGCCTCATTCACAAGCAAC-3’1000 C55’-CCCATTGTTGCTTGTGAATG-3’5’-TCCTCCATTGGCTTTGTCTC-3’603 C65’-ATCAGTCCCACTGCCACTTC-3’5’-CACCAGTGGGAGGAAGAAAG-3’992 C75’-CGGTGACAATGGGTATCCTC-3’5’-AGGCAAGCTCTTGGGAAAAG-3’933 C85’-TAAATGGGCCAATGAAGACC-3’5’-ACCCTCACTGCCTATCATGC-3’998 C95’-CCTAGACGGTGGAAGTCAGC-3’5’-GCCTTGTCTTCTTGCTTTGG-3’986 Supplementary table 3: mapping bsd deletion 1/4

Primers to map bsd deletion points on genomic DNA NameForwardReverse Amplicon size (base pairs) C105’-GGTGAATGGGATGAGCTAGG-3’5’-TCTTGTGGGATAGCCACATTC-3’884 C115’-CACATGTGGGAAAAGTGGTG-3’5’-AAGGACCTGAAGGGGTTAGC-3’885 C125’-TCAAATGTTGCCTCCTTTCC-3’5’-CGGCTGTGCTGAGTAAGATG-3’912 D15’-CAGCCTCAAGGACAGAGACC-3’5’-TCCAGCACAGTTGACGAAAG-3’922 D25’-GTCTGCCTTGAGGACTCTGC-3’5’-TGACACAGGCTGGAAGAATG-3’855 D35’-CCTCTGCTCACTCGAGAACC-3’5’-TGGTCTGTTGGCACAGAGTC-3’918 D45’-GCTAGCTGGAGAGCGTTGAG-3’5’-ATACGTTGGCATGGCTTTTC-3’955 D55’-CTGGCAGGCTTTCTTCACTC-3’5’-TTGGGTTTGTCGTTTCACTG-3’696 D65’-GACTAAAGCGAGGGCTTCTG-3’5’-TTCTGCCTTGTGTCTTCTGC-3’661 D75’-TCCTGTTTAGAGCGGCAGAG-3’5’-AGCCAGAGAAGCCTGAGATG-3’605 D85’-TCTCTGGCTGAGAACTGTGC-3’5’-CCCTGGACTTTGACTTTCTTG-3’844 D95’-TGTGGATGAACTCTGCCAAG-3’5’-GCCGTGTTTCAACTCTTTCC-3’671 D105’-AGGGGAAAGAAAGGCTTCTG-3’5’-AGATTGGGGATGTTTTGCAG-3’624 2/4

Primers to map bsd deletion points on genomic DNA NameForwardReverse Amplicon size (base pairs) D115’-GAAGCTAGCCAGCACAACTG-3’5’-AGAAGGCCCAGGATTAAAGG-3’654 D125’-CTCCAAAGTGCTTGGCTCTC-3’5’-TTGGGGGTAGATTGTGGAAG-3’765 D135’-GTATCCCAGCAAAGCAAAGG-3’5’-TGTATTGAGCCCCTCTGTC-3’817 D145’-TTACGGCTCACTGAAACTGC-3’5’-ACTGCACAGCCTCCCATAAG-3’811 D155’-CTAACCATGGGGGACACATC-3’5’-AGCTGCACCTTACTCCCTTG-3’261 E15’-TTCCTGACTGAGCCTTCTCC-3’5’-AGGGGGCTATAGGAGCTCTG-3’852 E25’-AGGGAGCAGGGATTTAAGGA-3’5’-AGGACCTAGGTTTGGGGTGA-3’868 E35’-GGCTGAACAGACCAACCATT-3’5’-GGAAAATTGCCTGGAATCAA-3’914 E45’-CAGGTGGCATTCTCGATTTT-3’5’-AACAAACACTGGGTGGAAGG-3’868 E55’-TTTCTGAAGGCAGGCTGTTT-3’5’-TGCTGAAAGGACCCAGATAGA-3’940 E65’-GTATGGGGACGTTGCTGTGT-3’5’-CCATATCCGGGGATCCAT-3’621 E75’-GATGGATCCCCGGATATG-3’5’-AAGAGGACAGAGCCAGCAGT-3’788 E85’-CCCAGGAGAGAAATTGATGC-3’5’-TCATGCCTTGATCTGCACTC-3’640 3/4

Primers to map bsd deletion points on genomic DNA NameForwardReverse Amplicon size (base pairs) E95’-TGGGGTGGTATTGTTGTTCC-3’5’-GGCCATGCTAACAGACCTTC-3’829 E105’-AAGAGGGCTTGTCATTTGG-3’5’-AGCGGCGATTTATCTAAGTTTG-3’849 E115’-CTGAGAGATGGGCAAAGAGG-3’5’-TGCCTACCGGTCAGAAATGT-3’714 F15’-CCACTCCAGAATGCATGAAA-3’5’-CCTCTCTGTTCCTTGGGTGA-3’909 F25’-ACACAGCCAGTGAGGTGACA-3’5’-CATTTCTGGAATGGGTGGAT-3’935 G15’-CTGGAGACAGCTGGCACATA-3’5’-AAAAGGAGGCTGAGGTAGGC-3’944 G25’-GGAGCGAGACACAAGGAGAC-3’5’-CATGCGCAGAAGACAACATT-3’919 4/4