Mosquitoes Bite How to genetically engineer the Anopheles mosquito to be resistant to Plasmodium.

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Presentation transcript:

Mosquitoes Bite How to genetically engineer the Anopheles mosquito to be resistant to Plasmodium

Plasmodium Parasite that causes Malaria

Anopheles Life Cycle

Cellular Target 2 places –Salivary Gland (parasite exits) –Gut (parasite grows)

Immunity Gene Scientists have found multiple genes in mosquitoes that make it resistant to Plasmodium One of these is the Rel 2 gene which is located in the gut of the mosquito It is deactivated by the Caspar gene

Isolating the Gene 2 possible methods –Reverse Transcriptase –Polymerase Chain Reaction …is a DNA polymerase enzyme that transcribes single-stranded RNA into double-stranded DNA. PCR Video

Construction Plasmid Immune Gene (Isolated with Restriction Enzymes) Green Gene New Plasmid!

Insertion Microinjection. Zygote Genetically engineered plasmid with Rel 2 gene and green gene