Performing HIV Rapid Tests 2005 Performing HIV Rapid Tests Demonstration and Practice Module 9: Performing HIV Rapid Tests

Learning Objectives At the end of this module, you will be able to: 2005 Learning Objectives At the end of this module, you will be able to: Perform 3 HIV rapid tests according to SOP KHB Rapid HIV 1/2 Stat-Pak HIV1/2 Uni-Gold HIV1/2 Perform multiple tests simultaneously Accurately interpret individual test results Accurately determine HIV status Module 9: Performing HIV Rapid Tests

Optional hands-on session Content Overview 2005 Overview of Testing Procedures Workspace Setup Practice (Multi-test algorithm with blind samples) Demo + Practice (individual tests on known samples) Optional hands-on session Module 9: Performing HIV Rapid Tests

2005 KHB rapid Module 9: Performing HIV Rapid Tests

Make ready the device and label with code number 123 Test line Control Line Sample port 5 5

KHB: Specimen collection 6

Add 40 l of whole/plasma/serum 7

Add 1 drop of running buffer 123 8 8

Test interpretation (within 30 minutes) Positive Test 123 9

Test interpretation Negative Test 123 10

Test interpretation Invalid result 123 11

The whole procedure (30’) 12

STAT-PAK

STAT-PAK : Getting Ready 2. Remove device from package, place it on flat surface and label device with client identification number 1. Collect test items and other necessary laboratory supplies .

STAT-PAK: Collecting Specimen 3. Fill the 5µl loop provided with the kit with whole blood, plasma or serum

STAT-PAK: Adding Specimen and Reagent to Test Device 4. Holding the sample loop vertically apply the 5µl of sample to the Sample pad (S) 5. Add 3 drops (150 µl) buffer slowly, drop wise into the Sample (S) well

STAT-PAK: Getting Results 6. Read the results between 15 and 20 minutes after addition of running buffer 7. Record results and other pertinent information on the worksheet

Stat-Pak: Test Interpretation Non-reactive Reactive Invalid

2005 Uni-Gold Module 9: Performing HIV Rapid Tests

Uni-Gold: Getting Ready 2005 Uni-Gold: Getting Ready 2. Remove device from package and label device with client identification number 1. Collect test items and other necessary lab supplies Module 9: Performing HIV Rapid Tests

Uni-Gold: Collecting Specimen 2005 Uni-Gold: Collecting Specimen 3. Collect specimen using the disposable pipette Module 9: Performing HIV Rapid Tests

Uni-Gold: Adding Specimen and Reagent to Test Device 2005 Uni-Gold: Adding Specimen and Reagent to Test Device 4. Add 2 drops (approx. 60µl) of specimen to the sample port in the device 5. Add 2 drops (approx. 60µl) of the appropriate wash reagent to sample port Module 9: Performing HIV Rapid Tests

Uni-Gold: Getting Results 2005 Uni-Gold: Getting Results 6. Wait for 10 minutes (no longer than 20 min.) before reading the results 7. Read and record the results and other pertinent info on the worksheet Module 9: Performing HIV Rapid Tests

Uni-Gold: Test Interpretation 2005 Uni-Gold: Test Interpretation Reactive Non-reactive Invalid Module 9: Performing HIV Rapid Tests

Activity: Workspace Setup 2005 Activity: Workspace Setup Instructions: Identify your Workspace in the practical room Gather test kits and supplies Obtain reactive and non-reactive specimens from instructor Arrange all items at your work station Activity time: 10 minutes Module 9: Performing HIV Rapid Tests

Hands-On Practice: Individual Tests 2005 Hands-On Practice: Individual Tests Instructions: Use safety precautions Practice with blood provided by your instructor only Raise your hand if you need additional supplies Show your test results to instructor after you are done Total time: 15 minutes per test Module 9: Performing HIV Rapid Tests

National Algorithm: Ethiopia 2005 Blood Sample Test 1 (KHB rapid ) Reactive Non-reactive Report Negative Test 2 (Stat Pak) Non Reactive Reactive Report Positive Test 3 (Unigold) Reactive Result Report Positive Non-reactive Result Report Negative Lab workers Health workers Counselors Module 9: Performing HIV Rapid Tests

Possible Outcomes in a serial Algorithm 2005 Possible Outcomes in a serial Algorithm KHB rapid Stat-Pak Uni-Gold HIV Status Non-reactive Negative Reactive Positive Invalid test needs to be repeated Does not count toward outcome of test Lab workers Health workers Counselors Module 9: Performing HIV Rapid Tests

Hands-On Practice: Multi-Test Algorithm 2005 Hands-On Practice: Multi-Test Algorithm Determine the HIV status for each of the three blind specimens provided by following your country’s serial algorithm 1 Test 1 - Test 2 - Test 3 HIV Status 2 3 4 5 Specimen Module 9: Performing HIV Rapid Tests

Hands-On Practice: Multi-Test Algorithm (Cont’d) 2005 Hands-On Practice: Multi-Test Algorithm (Cont’d) Instructions: Collect supplies and obtain panel of blind specimens Organize your workspace Complete one algorithm before starting the next Don’t forget – Use safety precautions Practice on blood provided by your instructor only Raise your hands if you need additional supplies Record results on worksheet Keep test devices - instructor will check results before discarding of devices Total time: 2 hours Module 9: Performing HIV Rapid Tests

Summary Describe the key learning from performing: 2005 Summary Describe the key learning from performing: KHB rapid HIV 1/2 Stat-Pak HIV 1/2 Uni-Gold HIV ½ Describe the key learning from performing the multi-test algorithm Module 9: Performing HIV Rapid Tests