Lab Equipment MicroscopeMicroscope Slide Cover SlipTest tube Test tube Rack Dissecting PanDissecting Scissors Dissecting PinsForceps PipetPetri DishWatch Glass Erlenmeyer FlaskBeaker Graduated Cylinder Magnifying Glass Safety Goggles

Microscope Eyepiece Arm Stage Fine adjustment knob Course adjustment knob Objective Nose piece Diaphragm Body Tube Base

Warm-up: August 10, 2012 Write down and try to answer the following questions: What is the function of the Microscope's Diaphragm? What would be the total Magnification of a Microscope, if its objective lens was 10x magnification? What does the prefix Micro- mean?

Warm-up: August 10, 2012 Complete the Measuring Volume Handout

Types of Microscopes Light Microscope (Compound Microscope)‏ Can be used to view living objects. Natural Color and movement can be observed. Poor Resolution- 200nm Easy to use; fairly inexpensive Electron Microscope Objects can not be alive Black and white images with no movement Excellent resolution- 0.2nm Viewer must be highly trained; very expensive.

Compound Microscope vs. Electron Microscope

2. Arm – provides support for the body tube 3. Eye piece – contains a lens that magnifies 4. Body Tube – allows light to pass up to the eye and provides the proper distance between the eyepiece lens and the objective lens. 5. Nose piece – holds the objectives and rotates to change magnification.

6. Objective Lens 1. High-power obj. lens (longest) provides the highest magnification 2. Medium-power obj. lens 3. Low-power obj. lens (shortest) provides the lowest magnification 7. Stage Clips – holds microscope slide in place. 8. Stage opening – allows light to pass through the slide

9. Stage – the platform where a slide is placed to be viewed 10. Diaphragm – regulates the amount of light that passes through the stage. 11. Light or mirror – provides or reflects light up through the specimen on the slide to the eye. 12. Base – supports the entire microscope 13. Fine adjustment Knob – used to bring the specimen into sharp focus 1. Coarse adjustment knob – used to focus the microscope

Magnification A light microscope has several lenses that magnify what you are viewing, which is why it is sometimes called a compound microscope. To find out how much magnification is being used, you first look at the eyepiece. It should be marked with a number such as 10x. This number means that the lens in the eyepiece magnifies 10 times. Now look at the low power objective. It is also marked with a number that tells its magnification. To find the total magnification being used, you multiply the two numbers.

Example1: The eyepiece of a light microscope is marked 10x and the low power objective is marked 4x. What is the total magnification? 10x * 4x = 40x

Example 2: The eyepiece of a light microscope is marked 20x and the low power objective is marked 5x. What is the total magnification? 20x * 5x = 100x

Example 3: The eyepiece of a light microscope is marked 100x and the low power objective is marked 60x. What is the total magnification? 100x * 60x = 6000x

Example 4: The eyepiece of a light microscope is marked 20x and the low power objective is marked 15x. What is the total magnification? 20x * 15x = 300x

Example 5: The eyepiece of a light microscope is marked 150 and the low power objective is marked 25x. What is the total magnification? 150x * 25x = 3750x

Closure What is the function of the Microscope's Diaphragm? What would be the total Magnification of a Microscope, if its objective lens was 10x magnification? What does the prefix Micro- mean?

What do I need to Study for My Quiz on Friday 1. Identifying Lab Equipment 2. Identifying Lab Symbols 3. Identifying Parts of the Microscope 4. Know the function of the Parts of the Microscope 5. Calculate Total Magnification