The stem cell niche in the Drosophila testes Hub

de Cuevas and Matunis 2011 Fig 1

de Cuevas and Matunis 2011 Fig 2

The JAK/STAT signaling pathway Arbouzova and Zeidler, 2006 Development

GSC Maintenance and Replacement There is a balance between stem cell loss and stem cell replacement. Lost GSCs can be replaced by.. 1)symmetric division where 1 GSC produces 2 GSC daughters 2)reversion of a differentiating cell to a GSC Remarkably, the reverting cells do not need to contact the hub at the time of reversion but may need to contact CySCs. de Cuevas and Matunis 2011 Fig 3

Adherens Junctions

Inaba et al., 2010 Fig 1 Dominant negative E-cadherin (E-cad dCR4h )

Regulation of Galactose Metabolism in Yeast Three genes are required to metabolize Galactose. Upstream Activating Sequence (UAS) These genes are activated in the presence of Galactose and repressed in the absence of Galactose. Russell, igenetics, 2 nd ed.

Regulation of Galactose Metabolism in Yeast Gal80p binds to the Gal4 AD and blocks activation Russell, igenetics, 2 nd ed.

Regulation of Galactose Metabolism in Yeast Gal3p changes Galactose to an unknown inducer form

Heterologous Promoter  Misexpression Experiment Alberts, Mol. Biol. Cell, 5 th ed.

A tissue-specific or time-specific promoter can be used to regulate GAL4 expression A gene of interest can be misexpressed by using a UAS transgene

Inaba et al., 2010 Fig 1

Inaba et al., 2010 Fig 1

Inaba et al., 2010 Fig 2

Centrosome with 2 Centrioles Centrioles are present in animal cells – not in higher plants or fungi.

γ-Tubulin molecules are present at centrosomes and stabilize the - end of MTs γ-Tubulin ring complexes (γ-TuRCs) anchor the MTs.

Inaba et al., 2010 Fig 2

Inaba et al., 2010 Fig 3

Inaba et al., 2010 Fig 4