1 Increment 33/34 JAXA Utilization Overview Payload Operations Integration Working Group (POIWG) #32 in Huntsville, July 24th – 26th, 2012 Takefumi Sakaguchi / Inc 33 Lead JEM PAYLOADS Yuji Abiru / Inc 34 Lead JEM PAYLOADS Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA).

2 JAXA Payloads Complement Overview JAXA Payload Ops Structure and Members - JFCT Staffing Trial for JEM Payloads Increment Overview - Final OOS Input Summary - Inc33/34 JAXA Utilization Theme - Inc35/36 JAXA Utilization Theme Contents

3 Topology in KIBO (JPM) - Saibo Rack and Ryutai Rack were launched on Flight 1J/A. - MELFI-1, ER4 and ER5 have been transferred into JPM during Inc MELFI-3 was launched on Flight 19A and then transferred into JPM. - MELFI-2 was swapped with MELFI 3 and transferred from USLab to the JPM during Inc Kobairo Rack and MSPR were launched on HTV-2 to ISS. Saibo Rack JPM1A2 Starboard Port Aft Forward Ryutai Rack JPM1A3 ER 5 JPM1F1 ER 4 JPM1F5 MELFI-1 JPM1D4 MELFI-2 JPM1A1 MSPR JPM1A4 Kobairo Rack JPM1F3 MSPR: Multi-purpose Small Payload Rack ER: Express Rack

4 Topology in KIBO (JEF) - SEDA-AP and MAXI were launched on 2J/A. - SMILES and HREP were launched on HTV-1. Multi-mission Consolidated Equipment (MCE) We are now operating three JAXA Exposed Payload Facilities (SEDA-AP, MAXI and SMILES) in JEF. In addition, NASA Exposed Payload Facility (HREP) is also in operations in JEF. HTV-3 will bring up MCE to JEF.

5 Background ✓ To achieve both increasing utilization mission success in the JEM and promotion of streamlining on JFCT structure, we continue the new JFCT staffing trial for JEM payload operations from Inc 29/30. Basic Concept ✓ When JEM PAYLOADS is off-console, J-Flight will complement the function. - The same way as we have been already doing in special holiday. (ex. New year holiday) - J-Flight will use JEM PAYLOADS/POD loop when coordinating with POD as JEM PAYLOADS does. ✓ When JEM PAYLOADS is off-console? - Weekend orbit 1&2 and only when JAXA exposed facility operations are planned in addition to NASA payload ops in JEM. - This trial has started from October JFCT Staffing Trial for JEM Payloads (1/3) JAXA Payload Ops Structure

6 User Integrator J-FLIGHT POICSSIPC/MCR SSIPC/UOA Rack Operator (BIO, FISICS, etc) Technical Support* JEM Pressurized Payload Operations NASA/POIC Cadre POD JAXA System/Payload Safety * SSIPC/ESR JEM Payload Officer (JEM PAYLOADS) Payload Ground System Officer* *IPU-G Operator *On-call JEM Exposed Payload Operations Exposed Facility Payload Officer (ExPO) MAXI Operator SEDA-AP Operator SMILES Operator Technical Support* (SEDA-AP, MAXI, SMILES) Payload Stowage Officer (HAWK-I)* JAXA PL FCT JFCT (FLAT,CANSEI, J-PLAN, J-COM, Tsukuba-GC, ARIES, KIBOTT) JAXA Payload Ops Conductor (JPOC) * Rack Officer (BIO, FISICS, GOLEM,MEISTER, etc) JFCT Staffing Trial for JEM Payloads (2/3) JAXA Payload Ops Structure

7 User Integrator J-FLIGHT POICSSIPC/MCR SSIPC/UOA Rack Operator (BIO, FISICS, etc) Technical Support* JEM Pressurized Payload Operations NASA/POIC Cadre POD JAXA System/Payload Safety * SSIPC/ESR JEM Payload Officer (JEM PAYLOADS) Payload Ground System Officer* *IPU-G Operator *On-call JEM Exposed Payload Operations Exposed Facility Payload Officer (ExPO) MAXI Operator SEDA-AP Operator SMILES Operator Technical Support* (SEDA-AP, MAXI, SMILES) Payload Stowage Officer (HAWK-I)* JAXA PL FCT JFCT (FLAT,CANSEI, J-PLAN, J-COM, Tsukuba-GC, ARIES, KIBOTT) JAXA Payload Ops Conductor (JPOC) * Rack Officer (BIO, FISICS, GOLEM,MEISTER, etc) JFCT Staffing Trial for JEM Payloads (3/3)

8 KIBO Payload Flight Control Team (PL FCT) JFCT in Misson Control Room (MCR) Payload FCT in User Operations Area (UOA)

9 JAXA Payload FCT Members (Inc 33/34) PL OPS MANAGER Chikara “Rickey” Harada Increment Lead Small Payload Officer (GNOME) Kuniko “Nicky” Okada Takefumi Sakaguchi Yuji Abiru Tetsuro Mizuno Yusuke Ohkawa Yuhei Suzuki Yasuyuki Sasaki Yukako Ishitsuka Shota Iino Mitsuhiro Kinno Kenichi Sekikawa Ryuji Hagi Hiroyuki Fujita ExPO (Exposed Payload Officer ) Norihiro Sakurai Naoya Adachi Takeshi Masuda Yoshihiro Koganemaru Yuki Wakayama MAXISMILES Yuki Matsui Junya Igaki SEDA-AP Kenji Sawahata Tsukasa Fujita Hideki Watanabe Kazuya Iwamoto Takashi Shimizu Reiko Sakai Susumu Fujita Hiromasa Saito Shin Ishida Kazuki Yanagisawa Junko Ohnishi Yuuta Iwahana Jun-ichi Yoshikawa Yuusuke Warashina GOLEM (GHF Ops Lead and Engineer of Material) Takashi Kihara Hiroharu Shibayama Hiroaki Miyata Hiroshi Tomioka Yasushi Kubota JEM PAYLOADS (JEM Payload Officer) Kyoichi Arakane Kensuke Teranishi Takuya Takami Kenji Noguchi Keiichiro “JJ” Sakagami Go Hyakutake Kuniko “Nicky” Okada Shunsuke Ochiai Takefumi Sakaguchi Yuji Abiru Futoshi Matsunaga BIO (Biology Ops Lead) Chie Inoue Yukako Ishitsuka Shohei Matsumoto Toshihiko Sasayama Shuichi Okawa Yusuke Suzuki JAXA PL Inc Scientist Izumi Yoshizaki Nobuyoshi Fujimoto Aiko Nagamatsu Waka Nishikawa Yoshimasa Higuchi Tsukasa Uekawa Payload Ground System Officer (PLGS) Kazumi Koide Takahisa Tokunaga JAXA IPM/IPE (JAXA Inc PL Manager /Engineer) JPOC (JAXA Payload Ops Conductor) Makoto Furukawa Yusuke Ohkawa Toru Mori Akane Yamaguchi Akiko Suzuki Tetsuhito Fuse IPU-G Kohei Noda Ritsuo Inoue Masaki Fukaishi HAWK-I (Payload Stowage Officer) Takahiro Sato Naoko Murakami Daichi Nakamura Atsushi Fujii Daisuke Masuda MEISTER (MSPR Engineering and Integration STaff for Enterprising Research) Tetsuro Mizuno Mei Sawada Naoki Ishikawa Kazue Ueyama Motoharu Masuda Hiroshi Okita BIO/FISICS/GOLEM/MSPR OP (Operator) Harumi Ugajin Mariko Ninomiya Naoya Nakate Hideki Nagata TBD FISICS (Fluid Science and Crystalization Science Ops Lead) Yukiko Abe Yuhei Suzuki Yuuta Iwahana Yasuyuki Sasaki Mariko Shimizu Shota Iino Hiroya Koike Inc33 Inc34 Naoki Ishikawa Shota Iino

10 Undock Dock JAXA Utilization Overview (Based on Final OOS Input) Increment 33/342012Sep.Oct.Nov.Dec.Jan.Feb.Mar. Note Saibo Rack 48P (- 12/25) 32S (10/17 - 3/18) 33S (12/7 - 5/16) 30S (- 9/17) Toal: 30 Runs Soyuz Progress 31S (7/17- 11/12) Ryutai Rack Marangoni Exp5 Nano Step Resist Tubule SpX-1 Kobairo Rack Hicari MSPR AQH Checkout C/O 51P (2/4 - 4/25) ATV COTS 50P (12/28 - 2/1) 49P (11/3 - 2/27) ATV3 SpX-2 (12/17 - 1/16) Orb-D (1/23 - 2/7) MEDAKA Osteoclast 50P 32S PCG SpX-2 31S SpX-1 (10/7 - 10/31)

11 Undock Dock JAXA Utilization Overview (Based on Final OOS Input) Increment 33/342012Sep.Oct.Nov.Dec.Jan.Feb.Mar. Note 48P (- 12/25) 33S (12/7 - 5/16) 30S (- 9/17) Soyuz Progress 31S (7/17- 11/12) 51P (2/4 - 4/25) ATV COTS 50P (12/28 - 2/1) 49P (11/3 - 2/27) ATV3 SpX-2 (12/17 - 1/16) Orb-D (1/23 - 2/7) Non Rack Biological Rhythms 48hrs Diagnostic Kit 2 Microbe-III Hair Area PADLES Commercial TEM for Orb Combination of Diagnostic Kit and Biological Rhythms 32S 32S (10/17 - 3/18) SpX-1 Stem Cells V-C REFLEX SpX-2 Orb-D 32S Pre/Post Flight Only SpX-1 (10/7 - 10/31)

12 Undock Dock JAXA Utilization Overview (Based on Final OOS Input) Increment 33/342012Sep.Oct.Nov.Dec.Jan.Feb.Mar. Note 48P (- 12/25) 33S (12/7 - 5/16) 30S (- 9/17) Soyuz Progress 31S (7/17- 11/12) 51P (2/4 - 4/25) ATV COTS 50P (12/28 - 2/1) 49P (11/3 - 2/27) ATV3 SpX-2 (12/17 - 1/16) Orb-D (1/23 - 2/7) EPO Unwinding Message in a Bottle JAXA VIDEO Try Zero G JAXA REPORT SEDA-AP MAXI Exposed Payload SMILES MCE Task List 32S (10/17 - 3/18) Blue Earth Gazing SpX-1 SpX-2 SpX-1 (10/7 - 10/31)

13 Inc33/34 JAXA Utilization Theme

14 Resist Tubule Research Objectives To clarify the mechanisms of gravity resistance in plants in the process from signal transformation and transduction to response. Operations Run1 (1-1 & 1-2): Microscope CCD camera and control pad are attached to fluorescence microscope in Clean Bench (CB) for observing Resist Tubule Chamber. Run3: Experiment Unit contains Plant Exp Sample Chamber and cultivates sample automatically for 32 days. KSC Fixation Tube (KFT) fixes samples. Crew activities: watering, set Meas Exp Unit B into CBEF, microscopic observation, sample fixation, MELFI insertion Time critical experiment. Facility/Interfaces: CBEF, CB, IPU, MELFI (+2 deg. C)

15 Ice Crystal 2 Research Objectives To study pattern, motion and growth rate of ice crystals in supercooled water including antifreeze glycoprotein (AFGP), and make clear mechanism of controlling crystal growth by AFGP. Ice Cell 2 Crew activities: Cell installation to SCOF and removal Facility/Interfaces: SCOF, IPU, MMA Experiment : 40 runs Crystal quality will be sensitive to g-disturbances. Experiment will be performed at GMT night time frame from ground. (Avoid docking time period or when there is a g-disturbing crew event in KIBO. ) Outputs - Temperature data (monitored by Ice Cell 2, downlinked via SCOF in real time) - Video Images of crystal (taken by Ice Cell 2, downlinked via IPU in real time) - Microgravity environment data (recorded by MMA, downlinked via LEHX or IPU) Examples of crystal images

16 MSPR Overview Multi-purpose Small Payload Rack (MSPR) is the rack to accommodate the researcher-made experiment equipment in JPM. MSPR provides Combustion Chamber for Experiment (CCE) which is used to conduct a combustion experiment. MSPR has interfaces of JEM Exhaust line, GN2 line and Medium Temp. Water line. These facilities are used inside of Work Volume (WV) which is the work space to be conducted combustion experiment and other experiments as well. MSPR consists of DC/DC Converter Unit (DCU), Multi Protocol Converter (MPC), Hub Unit (HBU), Video Compression and Recording Unit (VCRU), and CCE. Laptop PC is used by sending commands to experiment devices. Work Volume (WV) Small Experiment Area (SEA) Combustion Chamber for Experiment (CCE) Aquatic Habitat (AQH)

17 Aquatic Habitat (AQH) (1/2) Research Objectives During the space flight, bone mineral density is decreased by the influence of osteoclast activation, which molecular mechanism is expectantly investigated. To find the effect of microgravity on osteoclast in space, we perform a long-term experiment with small fresh water fish, medaka, and examine the alteration of osteoclast activity under microgravity with the histological analysis or the gene expression analysis by RNA in-situ hybridization. Schedule Medaka will be launched by 32Soyuz and 2months experiment will be started using the Aquatic Habitat. After approx. 2weeks and 2months from the experiment start, medaka will be sampled from the Aquatic Habitat Aquariums and chemically fixed for the retrieval. Also, the Aquatic Habitat maintenance operations, such as water sampling, water quality check, water exchange, and ORU exchanges are planned. Fixed samples will be retrieved by SpaceX-2 HTV332Soyuz 2 months SpX-2 Medaka (Oryzias latipes) Multi-purpose Small Payload Rack (MSPR) Aquatic Habitat (AQH)

18 Aquatic Habitat (AQH) (2/2) Crew activities: AQH C/O, experiment start, fish fixation, water quality check, water exchange. Facility/Interfaces: MSPR, MELFI(+2/-95 deg. C), CCD camera (HRDL) Medaka will be sampled from the Aquatic Habitat Aquariums and chemically fixed for the retrieval after approx. 2weeks, 1month, and 2months from the experiment start. The Aquatic Habitat maintenance operations, such as water sampling, water quality check, water exchange, and ORU exchanges are planned.

19 Stem Cells Research Objectives To estimate radiational effects to the organisms to forecast and defend the human body from those influences. The embryonic stem (ES) cells will be launched in the space by frozen condition. After about one month, six months, 12 months, 24 months, 36 months, the case will be retrieved to the ground. We will microinject the ES cells into mouse 8-cell embryos to develop and grow to adult mouse for analyzing the influence MELFI Stem Cells Sample Case A “Stem Cells Sample Case” in a mock up of MELFI 1/2 box module Cryo tubes in the Case Crew activities: MELFI ins. (Public Service) Facility/Interfaces: MELFI (-95 deg.C) After about one month, six months, 12 months, 24 months, 36 months, the case will be retrieved to the ground. Operations Once embryonic stem cells are launched at frozen condition, they’re kept in MELFI. Some dosimeters are stowed along with the samples and replaced within some extent interval. The case will be retrieved to the ground on a regular basis. (about 1, 6, 12, 24, 36 months).

20 EPO / Unwinding Research Objectives To clarify the differences between people’s senses of space in space and on the ground. The results would suggest ways of designing a comfortable living space in space and of improving the quality of life in space. These findings will also allow us to explore new ways of designing spaces on the ground. Operations Two set of G1 Camcorder will be fixed on the Multi-use Bracket at PORT side and STBD side. Two crew members will play catch by using the Ball at pre set position to relax in space, then evaluate the volumes and impressions of the spaces; JPM, JLP and around them. The Ball an example of set-up configuration Crew activities: Set up G1 Camcorders, play catch, evaluate the space and close out Facility/Interfaces: two G1 Camcorders

21 EPO / Message in a Bottle (MIB) Research Objectives To create a unique communication interface between space and earth, and present and future humankind. A small cylinder is to be filled with outer-space while the ISS is in orbit. It gives a chance for the participating astronaut to not only create a memento of his or her time in space but also a message for people on earth. Operations A crew will set the Bottle on the Platform for Small Sat Deploy. The Bottle will be exposed area through the JEM airlock. Once the Bottle is exposed in space, it includes and hold the vacuum automatically. Configuration of Bottle and Platform for Small Sat. deploy Bottle Crew activities: Set up G1 Camcorders, attach the Bottle to the Platform for Small Sat., take the video through JEM window and close out Facility/Interfaces: G1 Camcorder

22 EPO / Blue Earth Gazing Research Objectives Enjoy view of the Earth through water droplets from space, modeled on gracious moon gazing custom in Heian era in Japan. At the era, people enjoyed the moon reflected in a river or in cups of Japanese sake. Fig.1 Experiment configuration in JPM Fig.2 Image of the Earth through water droplets Operations One set of G1 Camcorder with Gazing Main Unit will be fixed on the Multi-use Bracket at Aft side of JEM Airlock and be located in front of JPM window. A crew will make some water droplets inside the Gazing Main Unit by using the water spray and take the video of the Earth through water droplets. Crew activities: Set up G1 with Gazing Main Unit on JEM airlock, make water droplets, take the video and closeout Facility/Interfaces: G1 Camcorder

23 Inc35/36 JAXA Utilization Theme

24 Material Exposure Equipment (ExHAM) (1) Overview Utilization Scenario Transfer ExHAM with samples to the exposed area through the JEM airlock, and install it to the handrail on JEM exterior by the JEMRMS small fine arm. Example Material exposure Capture of space debris/aerosols Small device test ExHAM : Exposed Experiment Handhold Attachment Mechanism

25 (2) Operational Scenario Material Exposure Equipment (ExHAM) (Cont’d)

26 (3) Handhold Candidates for ExHAM Material Exposure Equipment (ExHAM) (Cont’d)

27 (4) “Tanpopo”(dandelion) : the First mission for ExHAM Material Exposure Equipment (ExHAM) (Cont’d) Mission Objectives 1. Collection of Microbes from Space To analyze interplanetary migration of microbes, organic compounds, and meteoroids using Ultra low-density aerogel. 2. Survival of Exposed Microbes in Space To test the survivability of exposed microbes in space using sample tray.

28 There is no difficulty we can't overcome. Because “KIBO” is our hope !

29 Backup Charts

30 Payload Power Distribution Box (PPDB) P-PDB receives main power from a Power Distribution Unit and distributes power to JAXA ISPR Components and Experiment Facilities. AAA/SD Utility Interface Panel Utility Interface Panel provides following communication interfaces: 1553B, Ethernet, FDDI, and video. Maintenance Switch Panel (MSP) The MSP is used for redundant safety interlock. It is used to protect the crew during maintenance or installation activities. Utility DC to DC Converter Unit (UDC) UDC receives 120Vdc safing power from a Power Distribution Unit and provides 28V DC Power to experiment equipments such as Micro-gravity Measurement Apparatus. Clean Bench (CB) CB provides a closed work space for aseptic operation in order to perform life science and biotechnology experiments. Cell Biology Experiment Facility (CBEF) CBEF provides a controlled environment for studying fundamental phenomena of life in the space environment, using cells, tissues, small animals, plants, or microorganisms. PEF Access Port PEF Nozzle is penetrated into the PEF Access Port when the rack has fire. PEF Access Port for CBEF PEF Nozzle is penetrated into the PEF Access Port when the CBEF has fire. PEF Access Port for CB PEF Nozzle is penetrated into the PEF Access Port when the CB has fire. Saibo Rack Overview

31 Ryutai Rack Overview Protein Crystallization Research Facility (PCRF) AAA/SD UDC receives 120Vdc safing power from a Power Distribution Unit and provides 28V DC Power to experiment equipments such as Micro-gravity Measurement Apparatus. Utility Interface Panel Utility Interface Panel provides following communication interfaces: 1553B, Ethernet, FDDI, and video. Payload Power Distribution Box (PPDB) P-PDB receives main power from a Power Distribution Unit and distributes power to JAXA ISPR Components and Experiment Facilities. Image Processing Unit (IPU) IPU receives video data from cameras monitoring various experiments and from the Video Control Unit. IPU records, plays back, compresses, and outputs the data to the high rate data link for transmission to the ground. Solution Crystallization Observation Facility (SCOF) SCOF and PCRF provides opportunities for fundamental studies of crystal growth of various solutions and proteins. Fluid Physics Experiment Facility (FPEF) ) FPEF enables users to investigate the effects of the marangoni convection of the moderate temperature. Maintenance Switch Panel The MSP is used for redundant safety interlock during maintenance or installation activities. PEF Access Port PEF Nozzle is penetrated into the PEF Access Port when the rack has fire. Storage *Now FPEF silicon oil filter is installed on this position. Utility DC to DC Converter Unit (UDC)

32 Kobairo Rack Overview Gradient Heating Furnace (GHF) Water Pump Package (WPP) AAA/SD PEF Access Port Maintenance Switch Panel Payload Power Distribution Box (PPDB) Sample Cartridge P-PDB receives main power from a Power Distribution Unit and distributes power to JAXA ISPR Components and Experiment Facilities. PEF Nozzle is penetrated into the PEF Access Port when the rack has fire. The MSP is used for redundant safety interlock during maintenance or installation activities. The GHF is a vacuum furnace that contains three heating blocks. Their positions and temperatures can be independently controlled, and various temperature profiles can be realized. This facility will be mainly used for high quality crystal growth experiments using unidirectional solidification. Experiments can be performed automatically by installing 15 sample cartridges (Max.) in Sample Cartridge Automatic Exchange Mechanism ( SCAM). WPP is a pump package for secondary water coolant in Kobairo Rack.

33 SEDA-AP Overview RESEARCH OBJECTIVES SEDA-AP will measure space environment (neutrons, plasma, heavy ions, high-energy light particles, atomic oxygen, and cosmic dust) in ISS orbit and environmental effects on materials and electronic devices to investigate the interaction with and from the space environment at the JEM exposed facility (JEM EF). At the same time, it is also expected to conduct on-orbit verification of APBUS (Attached Payload BUS) technology, which furnishes necessary functions when mounted on the JEM EF.

34 MAXI Overview Gas Slit Camera (GSC) A slit camera with a gas proportional counter covers the 2 to 30 keV energy band with its large detection area. ·One-dimensional position-sensitive gas proportional counter with 10µm carbon anode wires, based on the method of charge division. ·MAXI is equipped with 12 counters with a total effective area of 5000 cm2. Solid-state Slit Camera (SSC) A slit camera with peltier-cooled X- ray sensitive CCD chips covers the 0.5 to 10 keV energy band with high resolution. ·The CCD chips are made in Japan. ·The pixel size is 24x24 µm, and there is a total of 1024x1024 pixels. ·MAXI is equipped with two cameras. One camera has 16 CCD chips, for a total effective area of 200 cm2. RESEARCH OBJECTIVES The Monitor of All-sky X-ray Image (MAXI) is an X-ray camera with a wide field of view designed to monitor active astronomical objects and the universe. It will monitor the variations of the X-ray intensity of various sources in the entire sky, at the highest sensitivity so far achieved with such an instrument.

35 SMILES Overview RESEARCH OBJECTIVES Superconduction Submillimeter-wave Limb-emission Sounder (SMILES), is aimed at global mappings of stratospheric trace gases by means of the most sensitive submillimeter receiver. Such sensitivity is ascribed to a Superconductor-Insulator-Superconductor (SIS) mixer, which is operated at 4.5 K in a dedicated cryostat combined with a mechanical cooler.

36 MCE Overview Outline of Payload Multi-mission Consolidated Equipment (MCE) accommodates five small mission experiment. 1. IMAP (ISS Ionosphere, Mesosphere, upper Atmosphere, and Plasmasphere mapping) earth observation mission consists from VISI (Visible-light and Infrared Spectral Imager) and EUVI (Extreme Ultra Violet Imager). 2. GLIMS (Global Lightning and Sprite Measurements) earth observation mission consists from cameras and electrical passive antennas. 3. SIMPLE (Space Inflatable Membrane Pioneer Long-term Experiment) technical demonstration mission consists from inflatable extendable mast and space terrarium. 4. REXJ (Robot EXperiment on JEM) robotic technical demonstration mission using tethers and extendable arm. 5. HDTV-EF (Commercial Off-The Shelf High Definition Television Camera System) technical demonstration mission using HDTV cameras.

37 ■ Design features (1/2) Material Exposure Equipment (ExHAM) – Attached to JEM JLP or JEF handhold by JEMRMS Small Fine Arm. – size:454 x 406 x 258 mm, weight: less than 22.5kg (50lbs)(including the samples) – The payload has no electrical device (for the first mission). – There are EVA devices for contingency EVA release capability, EVA bolt and Micro Conical Fitting.

38 ■ Design features (2/2) Material Exposure Equipment (ExHAM)

39 Inc35/36 JAXA Utilization Theme (Cont’d) Cell Mechanosensing Research Objectives To clarify the mechanism how the tension fluctuation in cell membrane regulate activities of such transducers during microgravity conditions. CBEF & Meas Exp Unit Meas Exp Culture Chamber in Cage with CO2 bag Meas Exp Solution Exchanger Operations The cells are launched at ambient using “Meas Exp CO2 bag” which maintain adequate concentration of CO2. After opening “Meas Exp CO2 bag” by a crew member, the cells are incubated using CBEF. After 3 days of incubation, 2 chambers are observed and fixed them using Cell Exp Fixation Cylinder, then kept at 2 deg.C in MELFI. Other chambers are continuously observed in next 4 days. Crew activities: set Meas Exp Unit into CBEF, sample fixation, MELFI insertion Time critical experiment. Facility/Interfaces: CBEF, IPU, MELFI (+2 deg. C)

40 Inc35/36 JAXA Utilization Theme (Cont’d) Space Pup MELFI Sample Case Ampoule (in the case) Research Objectives To examine the effects of cosmic radiation on spermatozoa using freeze-dried samples. These freeze- dried spermatozoa will be kept on board the ISS in Kibo and exposed to cosmic radiation for several months. Crew activities: MELFI ins. Facility/Interfaces: MELFI (-95 deg. C) After about 1 month, 12 months, 24 months, the samples will be retrieved to the ground. Operations Space Pup Sample Cases will be launched at ambient. They should be kept at -95C in MELFI on board for several months. On board operation is only to keep these samples at -95C in MELFI. The Sample Cases will be returned one by one at ambient.

41 Inc35/36 JAXA Utilization Theme (Cont’d) AQH Microscope Checkout Crew activities: Microscope assembly and C/O. Microscope disassembly and stow. Facility/Interfaces: MSPR, MWA, Ryutai UDC Microscope is assembled in the MSPR Work Volume or on MWA. CCD Camera Fluorescence LED Module Bright Field LED Module Condenser Scanning Stage Leica DMI6000B Base Stand Overview The Aquatic Habitat Microscope is an observation system that is a remotely controllable for various biological experiments, especially for aquatic experiments with Medaka etc. Initial checkout for subsequent science experiments will be performed in Increment 35/36.

42 Position (Call Sign) Roles and Responsibilities JEM Payload Officer (JEM PAYLOADS) JEM PAYLOADS is responsible for the overall JAXA payload complement, real-time operations, health and safety, and planning in cooperation with the EXPop. JEM PAYLOADS has overall authority for operations of NASA payloads located in JEM and operated by the POIC. JAXA Payload Ops Conductor (JPOC) JPOC supports JEM PAYLOADS and coordinates JAXA payloads planning, operations and anomaly resolution process. JPOC coordinates schedule conflict of NASA payloads in JEM with PPM. Rack Officer (BIO, FISICS, GOLEM, MEISTER) Small Payload Officer (GNOME) BIO/FISICS/GOLEM/MEISTER conduct JAXA pressurized payload racks (Saibo/Ryutai/Kobairo/MSPR) operations in coordination with JFCT and cope with troubles in the early phase. GNOME conducts JAXA non rack payloads operations such as educational, artistic, medical activities. Exposed Payload Officer (ExPO) ExPO conducts JAXA exposed facility payloads (SEDA-AP/MAXI/SMILES) Operations in coordination with JFCT and cope with troubles in the early phase. Operator (OP) Operators conduct telemetry monitoring and commanding on JAXA Payload Operations, following the instruction of Rack Officer or ExPO. JAXA PL FCT Roles & Responsibilities