PAPER NO: 144 International Conference on Advances in Chemical Engineering (ICACE 2015) NITK Surathkal, India – Dec 20-22, 2015 Evaluation of bio-surfactant.

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Presentation transcript:

PAPER NO: 144 International Conference on Advances in Chemical Engineering (ICACE 2015) NITK Surathkal, India – Dec 20-22, 2015 Evaluation of bio-surfactant mediated Microbial Enhanced Oil Recovery using sand packed column A Rajesh Kanna, Sathyanarayana N Gummadi and G Suresh Kumar Presenting author A. Rajesh Kanna Research Scholar Petroleum Engineering Program Department of Ocean Engineering Indian Institute of Technology Madras, India

Oil recovery mechanism Primary Pressure force 10 – 15 % recovery Secondary Water flood 20 – 30 % recovery Tertiary EOR 10 % recovery Chemical Thermal Microbial Others Leaching, Electrical Polymer Surfactant Bio-surfactant Ramakrishna Sen, Prog Energ Combust (2008), 34:714 - 724

Schematic MEOR http://www.teriin.org Commercial bio-surfactants: surfactin, lipopeptide, terhalose and rhamnolipid

Objectives To produce bio-surfactant using Bacillus subtilis MTCC 2422 To study the effect of temperature and pH on bio- surfactant production Bio-surfactant stability studies Application of bio-surfactant in oil recovery using sand column study

Samples after fermentation Bacteria in plate Production medium Incubation cum shaker Samples after fermentation Centrifugation Tensiometer

Effect of temperature on bio-surfactant production Cell dry weight (g/l) 1.7 Bio-surfactant (g/l) 1.4 Interfacial Tension (mN/m) 17

Effect of pH on bio-surfactant production Cell dry weight (g/l) 1.3 Bio-surfactant (g/l) 0.8 Interfacial Tension (mN/m) 18

Bio-surfactant stability studies Effect of pH Effect of temperature Effect of NaCl Effect of metal ions Bio-surfactant was stable at Basic pH Temperature 40 - 100 °C Salinity up to 50 g/l NaCl 20 g L-1 of metal ions

Oil Recovery - Sand column experiment Column packed with sand Column filled with oil Fluid injection - Water followed by Bio-surfactant Column packed with sand Column filled with oil Fluid injection - Water followed by Triton X 100 % Oil recovered Water - 28 Bio-surfactant – 11 Triton X 100 - 8

Conclusions Optimal conditions for Bacillus subtilis bio-surfactant production Source: Sucrose and Ammonium sulphate Temperature: 30 °C, pH: 7.0 Bio-surfactant was stable with temperature, NaCl and metal ions Bio-surfactant can recover 11% while Triton X 100 recovered 8%

Department of Ocean Engineering, IIT Madras Acknowledgements Department of Ocean Engineering, IIT Madras Department of Biotechnology, IIT Madras Dr. Indumathi N Nambi, Civil Engineering, IIT Madras Applied & Industrial Microbiology Lab members, friends and family ICACE 2015 organisation committee Session Chair Members

Thank You