PROTEIN SYNTHESIS From DNA to RNA to Proteins. Genes Section of DNA that controls making of proteins.

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Presentation transcript:

PROTEIN SYNTHESIS From DNA to RNA to Proteins

Genes Section of DNA that controls making of proteins

Protein Synthesis Overview 2 Main Processes –Transcription-DNA copied to RNA (nucleus) –Translation-RNA made into proteins (ribosomes)

DNA mRNA Transcription! RibosometRNA Amino acid Protein Translation!!!!

Types of RNA Messenger RNA (mRNA)- carries protein making instructions from DNA Ribosomal RNA (rRNA)- Part of the ribosome-makes proteins Transfer RNA (tRNA)- transfers amino acids to ribosome to make protein.

DNA vs. RNA RNA –Sugar (Ribose) –Phosphate group –Nitrogenous Bases A U (Not “T”) G C Single Stranded DNA –Sugar (Deoxyribose) –Phosphate group –Nitogenous Bases –A–A –T–T –G–G –C–C Double Stranded

Transcription 1. DNA is unzipped (by RNA polymerase-enzyme) at a gene. “Promoter” initiates copying. 2. ONE strand of the DNA template is transcribed (copied) into mRNA using complimentary base pairing. 3. RNA polymerase reaches “termination Signal”/end of gene. Stops copying.

ol/phbio/active_art/protein_synth esis/index.htmlhttp:// ol/phbio/active_art/protein_synth esis/index.html

Transcribe the following DNA strands. ATTCGACG UAAGCUGC TTACCAGC AAUGGUCG TTAAAACG AAUUUUGC

=ztPkv7wc3yUhttp:// =ztPkv7wc3yU Transcription video

Codon __3_ consecutive __bases_ on ___mRNA___ that specify _ particular amino acid.

FLOW OF GENETIC INFO Genetic Trait- Blue eyes

A C B NUCLEUS

Translation Video =B6O6uRb1D38&feature=relatedhttp:// =B6O6uRb1D38&feature=related

Translation -- The decoding of mRNA into a protein Nuclear envelope tRNA Amino acid Polypeptide chain Cell membrane

Transcription/translation video =NJxobgkPEAohttp:// =NJxobgkPEAo

How is Protein Synthesis like a candy factory?

yUYQ =B6O6uRb1D38&feature=relatedhttp:// =B6O6uRb1D38&feature=related =NJxobgkPEAohttp:// =NJxobgkPEAo

Translation Decode mRNA to Proteins

Steps of Translation 1. The mRNA strand is broken into codons –(Codon- 3 bases that code for an amino acids.)

Translation 2.Ribosome reads the codons and translates them into amino acids. How?? –Uses the Genetic Code –Match the first letter on the left –Match the second letter on the top –Match the third letter on the right –Ex: codon AUG – Amino Acid: Methionine

What amino acid goes with the following codons: UGG- GAA- ACA- UAG- AGC- CAG-

Example

3. rRNA sends for the tRNA to bring the correct amino acids. 4.The tRNA anticodons match up with the mRNA codons –Ex: mRNACUG-codon – t RNAGAC -anticodon brings the amino acid methionine attached to it. 5.Amino acids are attached to each other making a protein, until a STOP codon is reached

Translation continued 6. Disassembly- Ribosome complex falls apart. Polypeptide chain (protein) is released.

DNA: ACA TTG TAG CAT mRNA: AminoAcids: DNA: TTT TAC TGG CGC GTA mRNA: AminoAcids:

The bases below are DNA-Transcribe into mRNA, then translate to amino acids DNA= TAGCATTACACT mRNA=AUC-GUA-AUG-UGA Amino Acid=Ile-Val-Met-Stop

The bases below are DNA-Transcribe into mRNA, then translate to amino acids DNA= TTG-TCG-CGA-ACC-AGC-GGC-TAA mRNA=AAC-AGC-GCU-UGG-UCG-CCG-AUU Amino Acid=Asn-Ser-Ala-Trp-Ser-Pro-Ile