Amadori CML CEL MGH H O
PLGS identified AGE modified Peptides In HSA associated with CICs from Clinical plasma (Annotated MS/MS spectra)
A.Unmodified peptide B.Modified peptide C.Highlighted modified residue with “b-ions” or “y ions” encompassing the modified site. The lines (blue line for “b-ion and red line for y-ion) show the mass shift (∆M) corresponding to the type of AGE modification. Depiction of AGE modified MS/MS spectra
CON
A7 Sequence: RDAHKSEVAHR CML (5) A. B. C. ∆M ∆M (b5) = Da Da (CML)
CASLQKFGERAFK Carbamidomethyl C (1), CEL (6) Sequence: CASLQKFGERAFK Carbamidomethyl C (1), CEL (6) A. B. C Da (CEL) ∆M ∆M (b7) = Da
Sequence: NLGKVGSKCCK AMADORI (8), Carbamidomethyl C (9), Carbamidomethyl C (10) ∆M ∆M (y4) = Da Da (AMADORI)
IGT
Da (AMADORI) Sequence: LDELRDEGKASSAK AMADORI (5) A. B. C. ∆M ∆M (y11) = Da
Sequence: AEFAEVSKLVTDLTK CML (8) Da (CML) A. B. C. ∆M1 (b8) = Da ∆M Da (CML)
Da (CEL) Da (AMADORI) Sequence: NLGKVGSKCCK CEL (4), AMADORI (8), Carbamidomethyl C (9), Carbamidomethyl C (10) A. B. C. ∆M1 ∆M1 (y8) = Da ∆M2 ∆M2 (y4) = Da
NDD
Sequence: CASLQKFGERAFK Carbamidomethyl C (1), CEL (6) A. B. C Da (CEL) ∆M ∆M (y10) = Da
LSQRFPKAEFAEVSK MGH1 (4) ∆M ∆M (b5) = Da Da (MGH1)
Sequence: AEFAEVSKLVTDLTK CML (8) Da (CML) ∆M ∆M (b8) = Da A. B. C.
Sequence: DVFLGMFLYEYARR AMADORI (13) Da (AMADORI) ∆M ∆M (y2) = Da A. B. C.
Sequence: KQTALVELVK AMADORI (1) Da (AMADORI) ∆M ∆M (b1) = Da A. B. C.
DM
Sequence: RDAHKSEVAHR AMADORI (1), AMADORI (5) Da (AMADORI) ∆M1 ∆M1 (b2) = Da ∆M2 ∆M2 (b5) = Da A. B. C Da (AMADORI)
Da (CML) Sequence: ASSAKQRLK CML (5) ∆M ∆M (y5) = Da A. B. C.
Da (CEL) Sequence: CASLQKFGERAFK Carbamidomethyl C (1), CEL (6) A. B. C. ∆M ∆M (b7) = Da
Da (CML) Sequence: AEFAEVSKLVTDLTK CML (8) ∆M ∆M (b8) = Da A. B. C.
DM-MIC
F Da (CML) RDAHKSEVAHR CML (5) ∆M ∆M (y7) = Da A. B. C.
Sequence: AACLLPKLDELR Carbamidomethyl C (3), AMADORI (7) Da (AMADORI) ∆M ∆M (b7) = Da A. B. C.
Sequence: AEFAEVSKLVTDLTK CML (8) ∆M ∆M (b8) = Da A. B Da (CML) C Da (CML)
Da (CEL) Sequence: LKECCEKPLLEK Carbamidomethyl C (4), Carbamidomethyl C (5), CEL (7) ∆M ∆M (y6) = Da A. B. C.