KETA series Imaging System 2016/6/27 1 Gel Documentation (G) Multi-Purpose (M) Chemiluminescence (C)

Features of KETA series systems Robotic controlling system including: Motorized lens and filter wheel, auto sample stage(option) MC100/U10 LED: Multi color Epi-LED provides the basic fluorescence experiment. U10 white LED for sample placement. 2016/6/ Advanced, Automatic controlling system -

KD100 dark room: Absolute light-tight dark room suitable for chemiluminescence experiment. Leakage proof folding Optional SELM 200 Upgradeable high intensity Xenon light as excitation light of live plant and animal fluorescence detection. 2016/6/27 3 Safety inter lock Safety Switch Smart Latch AS100 Auto stage

Status display Signal light Customize idling time setting MD25K 312 nm UV Transilluminator (Also in KETA G) Intensity of UV light was controlled by computer Dedicate controlling software: Magic 1D & Magic Chemi 2016/6/27 4

5 KETA M Robotic Models: M: 2/3 ”, Peltier cooled ML: 2/3 ”, 2 stage Peltier cooled MLX: 2/3 ”, 16 bits, Peltier cooled Outstanding performance on gel documentation and highly sensitive on Chemi experiments.

KETA M KETA MLKETA MLX Gel Analysis Fluorescence Chemiluminescence /6/27 6

7 Camera of KETA M Series K12CHK12CHSK16CH CCD size 2/3" Depth 12/16. A/D bit 16 bit Grayscale 4096 / Resolution 1360X X 2048 S/N >62 dB>67 dB>69 dB Pixel Size 6.45 μm x 6.45 μm 7.4 μm x 7.4 μm Cooling Peltier2-stage PeltierPeltier ↓Ambient -30 ℃ -45 ℃ -40 ℃ Megapixels Communication USB 2.0

QE value 2016/6/27 8 Dark Current : K12CH/K12CHS/K16C/K16CH Lower than 20 ℃ Ambient K12CH/K12CH S K16C/K16CH

KETA M Configuration KETA M KETA MLKETA MLX CameraK12 CHK12 CHSK16CH Motorized Filter Wheel555 MC100 LED (W/R/G/B)Standard MC100-1 (W/G/B/UV 365 nm)Option AS 100Option Standard Magic1D & Magic ChemiStandard CommunicationUSB 2.0 Thermal printerOption SELM Module 200Option Lens f1.4, mm 2016/6/27 9

Typical Application for KETA M Gel documentation Colony counting Microtiter plate imaging Spot density calculation Photographic imaging 1 D analysis Fluorescence detection Chemiluminescence detection Optional SELM 200 for live imaging 2016/6/27 10

Sample Detection 2016/6/27 11 Protein Coomassie Blue Silver Stain SYPRO Orange SYPRO Ruby SYPRO Red Fluorescein Q-Dot Nucleic Acid Ethidium Bromide SYPRO Green SYPRO Gold Texas Red Gel Star Chemiluminescent ECL, ECL Plus ECL Advance, ECL Plex CDP star, Q-dot Super signal CSPD

Applications 2016/6/27 12 SYBR Green I stain: KETA M KETA ML Excited with: 312 nm Trans-UV, Filter: WK102 Excited with: 312 nm Trans-UV, Filter: WK105 SYPRO Red stain:

Chemiluminescence Experiment 2016/6/27 13 Sample: β-actin in MCF7 cell lysate Image model: ML Stain: plus-ECL (Perkin Elmer) Exposure time: 20 seconds Binning: Off Sample: GST pure protein Image model: ML Stain: ECL Enhanced (Millipore) Exposure time: 20 seconds Binning: Off (A) (B)

KETA M system: High performance gel doc and good sensitivity of Chemi detection. KETA ML system: 2-stage Peltier cooled system provides best cooling effect for the camera. ↓ noise, ↑performance.  Most profitable systems for sale. KETA MLX system: Truly 16 bits CCD with 4.2 Megapixels provides the best observation on both Chemi- and Fluor- detection. 2016/6/27 14

Specs to defeat competitors AS 100 sample stage 1. Easy for adjust the height of sample 2. Clear in focusing 3. Change samples fast and easy 4. Prevent cross contamination 5. Easy to enlarge the observation area 2 nd stage Peltier camera 1. Cheapest among the same level image systems 2. Absolute low temperature 3. Low noise 4. High S/N ratio 5. Good Chemi detection ability Upgradeable to live imaging system 2016/6/27 15

2016/6/27 16Magic1D Controls Dolphin series and KETA series, respectively

Features of 1D software Easy image capturing Multiple file format supported: JPG, BMP, JPEG, DFN, TIFF, PCX, GIF, TGA image Automatic data analysis (Within 1 minute) Traceable image enhancement function Intelligent colony counting Comprehensive microtiter plate analysis Accurate spot density calculation 3-D display image profile Support GLP/GMP Image blending function 2016/6/27 17

2016/6/27 18 Magic Chemi Software Enhancing on Image Capturing Tools Image capture control panel Batch capture for different exposure time DynaView capture function for accumulate multi-exposure signal Force cooling design ensure the best S/N ratio Darkroom calibration eliminate all undesired noise from the cabinet Binning and Gain function for hard-detectible signal

2016/6/27 19 Image Capturing Batch Capture: Allow users to select the best-desired image with optimal exposure time from the captured set. Time pic1 pic2 pic3 Exposure Time DynaView: Allow users to accumulate the multi capture signal with same exposure time. Time pic1 pic2 pic3 Exposure Time + + +

2016/6/27 20 Gel image analysis: DNA agarose gel, sequencing gel, blotting membrane, Protein SDS-PAGE gels, X-Ray film and photo etc. –Lane profile with lane image alignments –Ability to identify smiling gel accurately –Specialized X-axis band analysis function –Precise sample info report

2016/6/27 21 Colony counting: -Auto colony counting -intelligent colony distinguish Microtiter plate assay: Includes 48, 96, 384, 1536 wells micro-plate assay and various dot-blot and slot-blot formats.

2016/6/27 22 Spot image analysis: Suitable for irregular shape sample analysis, including 2-D gel sample and other special purpose assays.

Thank you!! 2016/6/27 23