Sample Collection Bayer recommendations BNP is unstable in glass containers Recommended sample type is EDTA plasma using plastic tubes for sample collection Average recovery of BNP in uncentrifuged whole blood after 24h storage at 2 to 8 o C = 96% After centrifugation store separated plasma samples at 2 to 8 o C Average recovery of BNP in EDTA plasma after 24h storage at 2 to 8 o C = 91% Samples are stable for up to 9 months when stored frozen at or below – 20 o C
Stability of BNP Plasma vs Whole Blood
Stability of BNP
Sample stability (plasma)
Effect of freezing and thawing