NASC European Arabidopsis Stock Centre: a quick overview ICAR2015

Location NASC began in 1990 with 200 stocks from Germany which were then passed on to start our sister centre ABRC in the US. In 2014, NASC and ABRC mirror nearly a million stocks.

a) Security – we preserve the seeds under the best possible conditions for longevity and germination frequency. b) Stability – any seeds donated can be recovered if local stocks get compromised or lost (or just lose their germination state) many years after donation. c) Publication – many journals now require seeds to be donated to the stock centres before publication. We give a NASC code on receipt of the seeds. d) Less work – we distribute on your behalf, and your name is associated with the donated stock forever. e) Publicity – we will associate your stocks with documents from your lab, e.g. instructions on use, observations etc, even logos if that is what you want. Benefits

Distribution NASC distributes over 100,000 tubes of seed a year

Two main centres NASC = Europe ABRC = USA, Canada and Latin America Rest of the world can go to either centre

Types of Stocks 6 ecotypes mapping populations characterised mutants multiple markers We hold ~1,000,000 genotypes 99%+ insertion lines knockouts RNAi knockdowns enhancer traps promoter traps transactivation lines activation tags

From A to B… Large donations in sufficient quantity can be released immediately We correlate orders with seed stocks; triple pack the seeds for GMO security against accidental release and then finally send the seeds to our users A sample of the original donation of seeds is dried to 5-7% moisture content and stored at -20°C as archival stock. Low quantity stocks are bulked (or re- bulked) under conditions of maximal separation from all other lines for quality control purposes Seed is catalogued and stored at 15% RH, 15ºC in our low humidity rooms Bulking Storage Distribution

Stocks sent GERMANY30089 CHINA28215 UK16548 SPAIN6151 FRANCE5657 SWITZERLAND3864 JAPAN3712 USA3678 BELGIUM3648 SINGAPORE3078 GERMANY26496 UK10246 SPAIN8994 CHINA7413 FRANCE5453 JAPAN5195 USA3707 SWITZERLAND2678 NETHERLANDS2439 BELGIUM GERMANY25504 CHINA20246 UK11409 FRANCE7413 USA7271 JAPAN4067 SWITZERLAND3912 DENMARK3765 NETHERLANDS3303 BELGIUM

Stocks sent 2014 New players

Additional uses Space Science at Royal Chelsea Flower Show Hampton Court Gardens

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Infrastructure Main laboratory

Glasshouse space Three glasshouses: Total space for 8000 lines per cycle 3 cycles per year = 24, 000 lines

Storage space -Six low humidity/temperature rooms which store the seed collection -Seed packets/tubes organized numerically -Orders are processed here SR1 SR2 SR6 (Newest)

The whole story… Donations are received and checked: GKsDUPLO Sowing Harvesting

Meet the Team Prof. Sean May Director Curation Bioinformatics NASC - ABRC Operations

Thank you Our funding body The Arabidopsis community Our staff: Chris, Andrew, Lamar, Siyue, Eliseo, Selman and Abdul

Donate now!