Phosphorylation by casein kinase 2 induces PACS ‐ 1 binding of nephrocystin and targeting to cilia by Bernhard Schermer, Katja Höpker, Heymut Omran, Cristina Ghenoiu, Manfred Fliegauf, Andrea Fekete, Judit Horvath, Michael Köttgen, Matthias Hackl, Stefan Zschiedrich, Tobias B Huber, Albrecht Kramer ‐ Zucker, Hanswalter Zentgraf, Andree Blaukat, Gerd Walz, and Thomas Benzing EMBO J. Volume 24(24): December 21, 2005 ©2005 by European Molecular Biology Organization

Nephrocystin is expressed in respiratory epithelial cells and accumulates at the base of cilia. Bernhard Schermer et al. EMBO J. 2005;24: ©2005 by European Molecular Biology Organization

Colocalization of nephrocystin with RPGR at the transition zone/basal body area of cilia in human respiratory epithelial cells. Bernhard Schermer et al. EMBO J. 2005;24: ©2005 by European Molecular Biology Organization

Nephrocystin colocalizes with the transport protein PACS ‐ 1 at the base of cilia of respiratory epithelial cells. Bernhard Schermer et al. EMBO J. 2005;24: ©2005 by European Molecular Biology Organization

Nephrocystin interacts with acidic cluster sorting protein PACS ‐ 1. Bernhard Schermer et al. EMBO J. 2005;24: ©2005 by European Molecular Biology Organization

The interaction between nephrocystin and PACS ‐ 1 is phosphorylation ‐ dependent and maps to a cluster of acidic residues in nephrocystin. Bernhard Schermer et al. EMBO J. 2005;24: ©2005 by European Molecular Biology Organization

Requisite phosphorylation of serines 121, 123, 126, but not serine 129, of nephrocystin mediates interaction with PACS ‐ 1. Bernhard Schermer et al. EMBO J. 2005;24: ©2005 by European Molecular Biology Organization

Targeting of nephrocystin to the transition zone/basal body area requires CK2 activity and is dependent on PACS ‐ 1. Bernhard Schermer et al. EMBO J. 2005;24: ©2005 by European Molecular Biology Organization