Date of download: 6/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anterior Keratocyte Depletion in Fuchs Endothelial Dystrophy Arch Ophthalmol. 2011;129(5): doi: /archophthalmol Identification of keratocyte nuclei from histologic sections stained with 4',6-diamidino-2-phenylindole. A, Sagittal section of a control cornea. B, The same sagittal section as in A, marked for counting. Keratocyte nuclei were counted using a manual point-and-click method. By convention, nuclei were not counted if they crossed the right boundary of the area defined by the yellow box. C, Sagittal section of a cornea with Fuchs endothelial dystrophy. The cornea was edematous with a thickened Descemet membrane and guttata. Keratocyte loss was visible in the anterior stroma. Scale bar, 100 μm; original magnification ×20. Figure Legend:

Date of download: 6/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anterior Keratocyte Depletion in Fuchs Endothelial Dystrophy Arch Ophthalmol. 2011;129(5): doi: /archophthalmol Division of full-thickness stroma into 5 layers. By confocal microscopy, mean keratocyte densities were determined from 2 manually selected frames (frontal section) from each of the 5 layers. By histology, each sagittal section was divided into the same 5 layers of stroma, and mean cell density in each layer was determined from 15 full-thickness sections that were each spaced by 8 μm. Figure Legend:

Date of download: 6/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anterior Keratocyte Depletion in Fuchs Endothelial Dystrophy Arch Ophthalmol. 2011;129(5): doi: /archophthalmol Mean (SD) keratocyte density determined by histologic and confocal methods. A, In corneas with Fuchs dystrophy (n = 11), keratocyte density was overestimated by automated assessment of confocal images in the anterior 10% of the stroma and underestimated by the confocal method in the posterior third of the stroma. B, In control corneas (n = 3), keratocyte density determined by automated assessment of confocal images did not differ from that determined by the histologic method. Data points are offset slightly for clarity. Figure Legend:

Date of download: 6/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anterior Keratocyte Depletion in Fuchs Endothelial Dystrophy Arch Ophthalmol. 2011;129(5): doi: /archophthalmol Mean (SD) keratocyte density determined by histologic methods in 5 stromal layers. Keratocyte density was lower in corneas with Fuchs dystrophy (n = 11) than in control corneas (n = 3) in all layers of stroma except for the middle third. Figure Legend:

Date of download: 6/29/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Anterior Keratocyte Depletion in Fuchs Endothelial Dystrophy Arch Ophthalmol. 2011;129(5): doi: /archophthalmol Confocal images of the anterior stroma in Fuchs dystrophy and control corneas. Few keratocytes were visible at the anterior boundary of the stroma in Fuchs dystrophy (A), although in control corneas this region of stroma has the highest density of stromal cells (B). The stromal boundary was approximately 15 μm posterior to subbasal nerves in both eyes. In patients with Fuchs dystrophy, this boundary was identified by the appearance of remnants of cells and cellular debris, whereas in control corneas, it was identified by the high concentration of cell nuclei. Cell density was also reduced 25 μm posterior to the stromal boundary in Fuchs dystrophy (C) compared with controls (D). Figure Legend: