By Iballa Martínez Yanes DT204/2 1

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-Beta-2-microbulin is a protein present in class 2 MHC molecules - Beta pleated sheet structure -Member of the immunoglobulin super family -It does not have a transmembranal region 3

4 Spondyloarthropathies: chronic inflamation of joints. Myolema: cancer of plasma cells in bone marrow DISEASES Renal filtration disorders

5 Blood samplesUrine samples Cerebrospinal fluid samples Plasma samples

- Measurement is based on level of agglutination - Agglutination occurs between beta-2- microglobulin and latex particles coated with antibodies. 6 Latex Antibodies Beta-2-microglobulin

7 Flow cytometer in the lab Principle

-Acchiardo S1, Kraus AP Jr, Jennings BR. (1989). Beta 2-microglobulin levels in patients with renal insufficiency.. Ad J Kidney Disease. 13 (1), p70-4 -Ackerman E, Rosevear JW. (1979). Immunonephelometric assays. Med Proq Technology. 6 (2), p American Association for Clinical Chemistry. (2014). Beta-2 Microglobulin Tumor Marker. Available: Last accessed 13th Feb Bernard, AM and Lauwerys, RR. (1983). Continuous-flow system for automation of latex immunoassay by particle counting. Clinical chemistry. 29 (6), p Bernard AM, Vyskocil A, Lauwerys RR. (1981). Determination of beta 2-microglobulin in human urine and serum by latex immunoassay..Clinical chemistry. 27 (6), p Charles A Janeway, Jr, Paul Travers, Mark Walport, and Mark J Shlomchik (2001). Immunobiology, The immune system in Health and Disease. 5th ed. New York: Garland Science. p Gooptu, M. (2014). Beta-2-microglobulin. Available: Last accessed 13th Feb

- Owen, J. Punt, J. Stranford and Stranford, S.A. (2009). The Major Histocompatibility Complex and Antigen Presentation. In: Champion, E. and Pech, I. Kuby Immunology. 7th ed. North America: Winslow, S.. p R Bataille, J Grenier, and J Sany. (1984). Beta-2-microglobulin in myeloma: optimal use for staging, prognosis, and treatment--a prospective study of 160 patients. Blood Journal. 63 (2), p R Bataille, M Boccadoro, B Klein, B Durie, and A Pileri. (1992). C-reactive protein and beta-2 microglobulin produce a simple and powerful myeloma staging system. Blood Journal. 80 (3), p Roberston, S. (2014). What is flow cytometry?. Available: sciences/What-is-Flow-Cytometry.aspx. Last accessed 12th Feb S D Khare, H S Luthra, and C S David. (1995). Spontaneous inflammatory arthritis in HLA-B27 transgenic mice lacking beta 2-microglobulin: a model of human spondyloarthropathies.. The Journal of Cell Biology (4), p