K. Martin KINGS AND QUEENS!!!

K. Martin RouterModesWANEncapsulationWANServicesRouterBasicsRouterCommands RouterModesWANEncapsulationWANServicesRouterBasicsRouterCommands DNA Tech Basics 1 DNA Tech Techniques Forensics Stem Cells Cloning Gene Therapy ► ► ► F i n a l Question ◄ ◄ ◄

K. Martin Answer Process where humans select the traits that are to be bred for in a particular species. DNA Tech Basics 100 ARTIFICIAL SELECTION or SELECTIVE BREEDING

K. Martin Answer KHOLARABI, BROCCOLI, BRUSSELS SPROUTS, CABBAGE, CAULIFLOWER, KALE Name one of the six vegetables that was bred for from wild mustard. DNA Tech Basics

K. Martin Answer HYBRID The offspring of two different species. DNA Tech Basics 300

K. Martin Answer BIOETHICS What field of biology studies the implications involved behind the decisions whether something is right or wrong? DNA Tech Basics 400

K. Martin Answer HUMAN GENOME PROJECT (HGP) Completed in 2003, this accomplishment sequences all 3.2 billion base pairs found in human DNA. DNA Tech Basics 500

K. Martin Answer POLYMERASE CHAIN REACTION (PCR) The process of alternating temperatures of a DNA sample to create billions of copies. DNA Tech Techniques

K. Martin Answer GEL ELECTROPHORESIS This technique separates molecules based on size. DNA Tech Techniques 200

K. Martin Answer RESTRICTION ENZYMES These proteins cut DNA at specific recognition sequences. DNA Tech Techniques 300

K. Martin Answer VIRUSES and PLASMIDS What are the two main types of vectors used in DNA technology? DNA Tech Techniques 400

K. Martin Answer FLUORESCENCE IN SITU HYBRIDIZATION (FISH) What technique uses a probe to light up the gene? DNA Tech Techniques 500

K. Martin Answer SHORT TANDEM REPEATS (STR) What is the region between the genes called where much of the variation between individuals takes place? Forensics 100

K. Martin Answer How many STRs does the FBI use to determine the likeliness of DNA profiles? Forensics 200

K. Martin Answer Male 1 Who’s the father? Forensics 300

K. Martin Answer TACGTC What are the first 6 nucleotides in this sequence of DNA? Forensics 400

K. Martin Answer % What percentage of human DNA codes for proteins? Forensics 500

K. Martin Answer TOTIPOTENT What type of cells can develop into any cell type? Stem Cells 100

K. Martin Answer INDUCED PLURIPOTENT STEM CELLS, (iPS CELLS) When somatic cells are turned back into stem cells, what type of cell do they become? Stem Cells 200

K. Martin Answer INNER CELL MASS (ICM) What region of the blastula/blastocyst do adult stem cells come from? Stem Cells 300

K. Martin Answer ECTODERM, MESODERM, ENDODERM What are the three germ layers of a gastrula? Stem Cells 400

K. Martin Answer MULTIPOTENTCY What type of potency can form the least amount of differentiated cell types but is still a type of stem cell? Stem Cells 500

K. Martin Answer GENE CLONING What type of cloning uses recombinant DNA vectors? Cloning 100

K. Martin Answer THE NUCLEAR DONOR In the creation of Dolly, what sheep is genetically identical to her? Cloning 200

K. Martin Answer SOMATIC CELL NUCLEAR TRANSFER (SCNT) or REPRODUCTIVE CLONING What process created Dolly? Cloning 300

K. Martin Answer THERAPEUTIC & REPRODUCTIVE CLONING What two types of cloning use the genetic material of a somatic cell and places it into a donor egg cell. Cloning 400

K. Martin Answer NONE In what country is human cloning legal? Cloning 500

K. Martin Answer DNA PROBES What are ssDNA molecules that detect certain genes called? Gene Therapy 100

K. Martin Answer IN VITRO FERTILIZATION (IVF) What is it called when a sperm fertilizes an egg in a test tube or petri dish? Gene Therapy 200

K. Martin Answer RADIOACTIVE ssDNA & FLUORESCENT ssDNA What are the two main types of probes used in genetic therapy? Gene Therapy 300

K. Martin Answer PREIMPLANTATION GENETIC DIAGNOSIS (PGD) What is it called when one cell of a morula is tested for a genetic disorder? Gene Therapy 400

K. Martin Answer RNAi What molecule can intercept an mRNA molecule before it reaches the ribosome to essentially “turn off” a gene? Gene Therapy 500

K. Martin Answer CHICKS WITH DYED FEATHERS, BREEDING GIANT PANDAS, PUREBRED DOGS, SHEARED WOOLY SHEEP, VEAL, ANY OTHERS THAT APPLY What bioethical issue discussed in class does not need DNA technology? Final Question