Gas Chromatography Gas Chromatography Effendy De Lux Putra

Gas Chromatography A physical separation method It involves the distribution of component between two phases –Stationary phase: solid (10%) liquid (90%) –Mobile phase: gas

Why use GC ? Short analysis time Wide Choise of Stationary Phase Wide Choice of Detectors Ease of operation

Goals To separate mixtures of organic compounds To detect individual analytes To identify & quantitative using reference standards Preparative work

Block Diagram of a GC Block Diagram of a GC Block Diagram of a GC Injector InjectorColumnDetector Data Processor Qualitative Analysis Quantitative Analysis

Typical GC System A GC comprised of five main parts –Flow Controller –Injection Port –Column and Column Oven –Detector –Data processor

Mechanism of Zone Migration Chromatography separation is due to the differential migration rate of components in the mixtures The mobile phase helps to move the sample through the stationary phase