BIO409/509 Cell and Molecular Biology

SECOND Methods paper assignment due Wed., 4/20 (you don’t do this assignment if you are in the 4H STEM Ambassador Program) Assignment: Choose a SECOND method in Molecular Biology or Cell Biology and write 2-3 page paper about the following: 1)Describe the steps done in the method 2)Explain the “highlight” steps: What are the key “tricks” in the method. 3)Describe two different applications of the method to help demonstrate how the method is valuable. On an extra page, include a bibliography with any resources you used: Our textbook, web sites, personal communications, journal articles, etc.

BIO409/509 Cell and Molecular Biology We will skip Chapter 7 DNA Replication and never cover it. Finish Chapter 8 RNA Synthesis on on to Chapter 9 Protein Synthesis. That’s the end of Part II. We’ll start talking about Chapter 10 The Nucleus this week.

Protein Synthesis, Processing, and Regulation 9

Figure 9.9 Overview of translation

Figure 9.8 Signals for translation initiation

Table 9.1 Translation Factors

Figure 9.11 Initiation of translation in eukaryotic cells (Part 1)

Figure 9.18 Translational repressor binding to 3' untranslated sequences

Translation of mRNA RNA interference is mediated by: Small interfering RNAs (siRNAs)— produced from double-stranded RNAs by the nuclease Dicer. MicroRNAs (miRNAs)—transcribed by RNA polymerase II, then cleaved by nucleases Drosha and Dicer.

Figure 9.13 Elongation stage of translation (Part 1)

Figure 9.13 Elongation stage of translation (Part 3)

Figure 9.2 Attachment of amino acids to tRNAs

Figure 9.21 Regulation of translation by phosphorylation of eIF2 and eIF2B (Part 1)

Figure 9.21 Regulation of translation by phosphorylation of eIF2 and eIF2B (Part 2)

Figure 9.15 Termination of translation (Part 2)

Figure 9.30 The role of signal sequences in membrane translocation

Figure 9.16 Polysomes (Part 2)

Figure 9.23 Action of chaperones during translation

Figure 9.25 Sequential actions of chaperones

Table 9.2 Representative Diseases Associated with Protein Aggregation

Protein Folding and Processing Prions are misfolded proteins that can self-replicate. Diseases caused by prions include scrapie in sheep, mad cow disease, Creutzfeldt- Jakob disease, and kuru.

Figure 9.24 Action of chaperones during protein transport