10/30/2013BCHB524 - 2013 - Edwards Project/Review BCHB524 2013 Lecture 17.

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Presentation transcript:

10/30/2013BCHB Edwards Project/Review BCHB Lecture 17

Class Project: Expectations 40% of your grade! Project Report Long version of your homework write-up Project Presentation Demo your program Describe your project solution 10/30/2013BCHB Edwards2

10/30/2013BCHB Edwards3 Class Project: Blast Database 1. Write a program that computes all pairwise blast alignments for two species' proteomes and stores the alignments in a relational database. 2. Write a program that retrieves the blast alignment for two proteins (specified by their accessions) from the relational database. 3. Write a program that finds pairs of orthologous proteins that are mutually best hits in the species' proteomes.

Notes 10/30/2013BCHB Edwards4

10/30/2013BCHB Edwards5 Class Project: MS/MS Viewer Write a program to display peptide fragmentation spectra from an mzXML file. The program will take an mzXML file, a scan number, and a peptide sequence as input. The peptide's b-ion and y-ion m/z values should be computed, and peaks matching these m/z values annotated with appropriate labels. The output figure/plot should aid the user in determining whether or not the peptide is a good match to the spectrum.

Notes 10/30/2013BCHB Edwards6

10/30/2013BCHB Edwards7 Class Project: Protein Digest Write a simple web-server application using TurboGears to carry out an in silico enzymatic digest of a user-provided protein sequence. Users should be able to specify min and max length, min and max molecular weight, # of missed cleavages, and specific enzyme. Output should be a table of peptides, with their length, molecular weight, # of missed cleavages, and amino-acids to left and right of each peptide in the protein sequence.

Notes 10/30/2013BCHB Edwards8