Date of download: 7/1/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Diagnosis of Intra-amniotic Infection by Proteomic Profiling and Identification of Novel Biomarkers JAMA. 2004;292(4): doi: /jama SELDI-TOF indicates surface-enhanced laser desorption/ionizationtime-of-flight. Group B Streptococcus infection– induceddifferential protein expression in nonhuman primate (A) and human (B) amnioticfluid samples. SELDI-TOF-mass spectrometry spectrum of amniotic fluid extractsbound to chemically defined Normal Phase Protein chip arrays. Spectrum from2.5 to 15 kDa collected at 235 laser intensity. Detailed spectra show increasedexpression of the 3.5-kDa and 10.8-kDa peaks between control and infected.Arrows indicate the unique peaks represented by polypeptides overexpressedin infection. Figure Legend:

Date of download: 7/1/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Diagnosis of Intra-amniotic Infection by Proteomic Profiling and Identification of Novel Biomarkers JAMA. 2004;292(4): doi: /jama Surface-enhanced laser desorption/ionization time-of-flight analysisof nonhuman primate amniotic fluid before and after infection using NormalPhase Protein chip arrays. The 10.8-kDa candidate biomarker is detected inamniotic fluid infected with Mycoplasma hominis and Ureaplasma parvum. Detection in group B Streptococcus is shown in Figure 1. Figure Legend:

Date of download: 7/1/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Diagnosis of Intra-amniotic Infection by Proteomic Profiling and Identification of Novel Biomarkers JAMA. 2004;292(4): doi: /jama A, Nonhuman primate control and infected amniotic fluid and humancontrol and infected amniotic fluid. VDBP indicates vitamin D binding protein,an unregulated control marker. Insulinlike growth factor binding protein 1(IGFBP-1) bands represent the intact protein (approximately 30 kDa) and proteolyticfragment (approximately 11 kDa). B, Detection of calgranulin B and IGFBP-1in pooled human maternal serum from immunoprecipitates. Figure Legend: