2004. 5. 25 Ji-Yoon Park Nanoparticle-Based Theorem Proving.

Slides:

Advertisements

Similar presentations

Introduction to perl programming: the minimum to know! Bioinformatic and Comparative Genome Analysis Course HKU-Pasteur Research Centre - Hong Kong, China.

Advertisements

Supplementary Figure S1 (A) Change of reporter activity levels after actinomycin D treatment. HEK293T cells were transiently transfected with the reporter.

Uses of Cloned Genes sequencing reagents (eg, probes) protein production insufficient natural quantities modify/mutagenesis library screening Expression.

The genetic code.

Center for Biological Sequence Analysis The Technical University of Denmark DTU Chromatin and Gene Expression in E. coli Dave Ussery Biological Sequence.

Center for Biological Sequence Analysis Prokaryotic gene finding Marie Skovgaard Ph.D. student

 -GLOBIN MUTATIONS AND SICKLE CELL DISORDER (SCD) - RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLP)

ATG GAG GAA GAA GAT GAA GAG ATC TTA TCG TCT TCC GAT TGC GAC GAT TCC AGC GAT AGT TAC AAG GAT GAT TCT CAA GAT TCT GAA GGA GAA AAC GAT AAC CCT GAG TGC GAA.

Supplementary Fig.1: oligonucleotide primer sequences.

Gene Mutations Worksheet

Transcription & Translation Worksheet

Crick’s early Hypothesis Revisited. Or The Existence of a Universal Coding Frame Axel Bernal UPenn Center for Bioinformatics Jean-Louis Lassez Coastal.

1 Essential Computing for Bioinformatics Bienvenido Vélez UPR Mayaguez Lecture 5 High-level Programming with Python Part II: Container Objects Reference:

Today… Genome 351, 8 April 2013, Lecture 3 The information in DNA is converted to protein through an RNA intermediate (transcription) The information in.

GENE MUTATIONS aka point mutations. DNA sequence ↓ mRNA sequence ↓ Polypeptide Gene mutations which affect only one gene Transcription Translation © 2010.

IGEM Arsenic Bioremediation Possibly finished biobrick for ArsR by adding a RBS and terminator. Will send for sequencing today or Monday.

Application of MLVA-15 genotyping for typing of Brucella abortus isolates from India Dr. Gita Kumari.

 The following material is the result of a curriculum development effort to provide a set of courses to support bioinformatics efforts involving students.

Nature and Action of the Gene

Introduction to Python for Biologists Lecture 2 This Lecture Stuart Brown Associate Professor NYU School of Medicine.

Math 15 Introduction to Scientific Data Analysis Lecture 10 Python Programming – Part 4 University of California, Merced Today – We have A Quiz!

Undifferentiated Differentiated (4 d) Supplemental Figure S1.

Supplemental Table S1 For Site Directed Mutagenesis and cloning of constructs P9GF:5’ GAC GCT ACT TCA CTA TAG ATA GGA AGT TCA TTT C 3’ P9GR:5’ GAA ATG.

Other Detection System of Theorem Proving Park, Ji-Yoon.

Lecture 10, CS5671 Neural Network Applications Problems Input transformation Network Architectures Assessing Performance.

GeneGenbank NumberSequences GFPU55762 Sense: 5’-GUU CAG CGU GUC CGG CGA GTT Antisense: 5’-CUC GCC GGA CAC GCU GAA CTT Rat TLR2NM_ Sense: 5’-GGA UCU.

Polyamine Oxidase is the Source of Hydrogen Peroxide in Plants

Fig. S1 siControl E2 G1: 45.7% S: 26.9% G2-M: 27.4% siER  E2 G1: 70.9% S: 9.9% G2-M: 19.2% G1: 57.1% S: 12.0% G2-M: 30.9% siRNF31 E2 A B siRNF31 siControl.

PART 1 - DNA REPLICATION PART 2 - TRANSCRIPTION AND TRANSLATION.

TRANSLATION: information transfer from RNA to protein the nucleotide sequence of the mRNA strand is translated into an amino acid sequence. This is accomplished.

Antibiotic Resistance and Strategies to Develop New Antibiotics D. J. Kalita Associate Professor Department of Veterinary Biochemistry Faculty of Veterinary.

 The following material is the result of a curriculum development effort to provide a set of courses to support bioinformatics efforts involving students.

NSCI 314 LIFE IN THE COSMOS 4 - The Biochemistry of Life on Earth Dr. Karen Kolehmainen Department of Physics CSUSB

TABLE 2 Oligonucleotide primers and probes used in FISH* S1PRO634RHLIProchlorococcus GCC GAT CAG TTT CCA CTG S2PRO634RHLIIProchlorococcus GCC TTT CAG.

The 3 rd Research on Theorem Proving MEC Meeting Hanyang University Proteome Research Lab Park, Ji-Yoon.

Prodigiosin Production in E. Coli Brian Hovey and Stephanie Vondrak.

Passing Genetic Notes in Class CC106 / Discussion D by John R. Finnerty.

Supplementary materials

Dictionaries. A “Good morning” dictionary English: Good morning Spanish: Buenas días Swedish: God morgon German: Guten morgen Venda: Ndi matscheloni Afrikaans:

Transcription and Translation Activity 1.You will work with the person sitting next to you. 2.One of you will take the role of RNA polymerase and transcribe.

The 3 rd Research on Theorem Proving MEC Meeting Hanyang University Proteome Research Lab Hanyang University Proteome Research Lab Park, Ji-Yoon.

Suppl. Figure 1 APP23 + X Terc +/- Terc +/-, APP23 + X Terc +/- G1Terc -/-, APP23 + X G1Terc -/- G2Terc -/-, APP23 + X G2Terc -/- G3Terc -/-, APP23 + and.

Oligonucleotide-based Theorem Proving by Cross-Linking Gold Nanoparticle Assembly Park, Ji-Yoon.

GENE EXPRESSION. Transcription 1. RNA polymerase unwinds DNA 2. RNA polymerase adds RNA nucleotides (A ↔ U, G ↔ C) 3. mRNA is formed! DNA reforms a double.

RA(4kb)- Atggagtccgaaatgctgcaatcgcctcttctgggcctgggggaggaagatgaggc……………………………………………….. ……………………………………………. ……………………….,……. …tactacatctccgtgtactcggtggagaagcgtgtcagatag.

Example 1 DNA Triplet mRNA Codon tRNA anticodon A U A T A U G C G

Topic: Replication of DNA Standard: Explain the role of DNA in storing and transmitting cellular information.

DNA, RNA and Protein.

Protein Synthesis Worksheet Answer Key

Figure S1. Construction of pAL70

Nanoparticle-based Theorem Proving

Modelling Proteomes.

Supplementary information Table-S1 (Xiao)

Sequence – 5’ to 3’ Tm ˚C Genome Position HV68 TMER7 Δ mt. Forward

Supplemental Table 3. Oligonucleotides for qPCR

GENE MUTATIONS aka point mutations © 2016 Paul Billiet ODWS.

Supplementary Figure 1 – cDNA analysis reveals that three splice site alterations generate multiple RNA isoforms. (A) c.430-1G>C (IVS 6) results in 3.

Huntington Disease (HD)

DNA By: Mr. Kauffman.

Tissue-Specific Reduction in Splicing Efficiency of IKBKAP Due to the Major Mutation Associated with Familial Dysautonomia Math P. Cuajungco, Maire Leyne,

Gene architecture and sequence annotation

Molecular engineering of photoresponsive three-dimensional DNA

Fundamentals of Protein Structure

Station 2 Protein Synethsis.

6.096 Algorithms for Computational Biology Lecture 2 BLAST & Database Search Manolis Piotr Indyk.

Shailaja Gantla, Conny T. M. Bakker, Bishram Deocharan, Narsing R

Presentation transcript:

Ji-Yoon Park Nanoparticle-Based Theorem Proving

May 25, 2004 APRL 2 Target Problem P ∧ Q → R, S ∧ T → Q, S, T, P R = True ?

May 25, 2004 APRL 3 DNA-Based Nanoparticle Assembly Strategy Mirkin, 2000

May 25, 2004 APRL Purchase of gold nanoparticle 2. Preparation of 3’-(alkanethiol) modified oligonucleotides 3. Preparation of Linker DNA 4. Characterization of DNA-linked gold assembly Experimental Section

May 25, 2004 APRL 5 PrimerSequence(5’ → 3’)LengthModification ST¬Q AAG CAG TAG CGA CCA ATT GAC GCA AAT TGA CGT ACG TAC GCT GAA 45 merNone ¬RPQ CAT ACA ATG AAC GCA GTC AAC GCA AGG CAG TTC AGC GTA CGT ACG 45 merNone ¬P CTG CCT TGC GTT GAC 15 mer 5’-phosphate R TGC GTT CAT TGT ATG 15 mer 3’-thiol ¬S TGG TCG CTA CTT 15 mer 3’-thiol ¬T TCA ATT TGC GTC AAT 15 mer 5’-phosphate The Sequence of Oligonucleotides

May 25, 2004 APRL 6 Assembly 15 mer 45 mer S1 Digestion Hypothesis

May 25, 2004 APRL 7 Colloidal Gold

May 25, 2004 APRL 8 Full Assembly vs S1 Digestion

May 25, 2004 APRL 9 Partial Assembly vs S1 Digestion

May 25, 2004 APRL 10 TEM Image (Colloidal Gold) (a)(b)(c) Figure 2. TEM image of the annealed DNA-linked assembly (a) Colloidal gold (b) Full-assembly of DNA-linked nanoparticles (c) S1 Nuclease digestion of full-assembled DNA-linked nanoparticles TEM image of the annealed DNA-linked assembly (a) Colloidal gold (b) Full-assembly of DNA-linked nanoparticles (c) S1 Nuclease digestion of full-assembled DNA-linked nanoparticles

Surface-Based Theorem Proving

May 25, 2004 APRL 12 Surface-based DNA Computation Liu et al., 2000

May 25, 2004 APRL Purchase of gold nanoparticle 2. Preparation of 3’-(alkanethiol) modified oligonucleotides 3. Preparation of Linker DNA 4. Characterization of DNA-linked gold assembly Experimental Section

May 25, 2004 APRL 14 I II III IV V A B C (a) (b) I II III IV V I II III IV V A B C (c) ¬RPQ ¬T ¬S P.C N.C R ¬P ST ¬Q ¬RPQ ¬T ¬S P.C N.C Theorem Proving by Fluorescence Imaging

May 25, 2004 APRL 15 Paper publish Genotyping experiment Structure & Theorem Proving Further Work