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Lecture 6 Web: pollev.com/ucibio Text: To: Type in:

Lecture 6 objectives Understand how 2° structure leads to 3° structure Know the different interactions that stabilize structure Understand domains, motifs and their relationship to structure Understand the theory of local energy minimas in folding Understand formation of 4° structure Relate protein structure to function, and disease

Folding proteins Need precise shape – active site Amino acid sequence – determines folding Secondary structural elements form

Motifs & Domains Motif: Common structural element Eg. Helix-turn-helix Will not fold independently

Motifs & Domains Domain:Common structure Eg. Ig domain Folds independently

Working together Quaternary structure Multiple peptide chains interact Structure & function dependent on interactions

Quaternary structure

Another protein folding experiment… APX Normal activity250 units450 units +Heat5 units9 units +Heat +recovery188 units14 units +Protein “Y”244 units412 units +“Y” + heat7 units11 units +heat +Y +recovery198 units388 units

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The importance of protein structure “Chaperones” & protein folding Misfolded proteins are very, very bad! E.g. Huntington’s disease

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The importance of structure “Chaperones” & protein folding Misfolded proteins are very, very bad! E.g. Huntington’s disease E.g. Prions

MC questions in exam Question text  T  F  ? A) Answer choice 1  T  F  ? B) Answer choice 2  T  F  ? C) Answer choice 3  T  F  ? D) Answer choice 4      Score ranges from -3 to +4

Practice exam Sent out No answers Discuss, Google, etc.