M P N HN M M P N HN M M P N HN M M P N HN M M P N HN M A Figure S1 A Strain CEPD 7 Strain NCPPB 790 Strain B03167Strain NCPPB 339Strain NCPPB 909

B M P N HN M M P N HN M M P N HN M M P N HN M Figure S1 B Strain CEPD 7 Strain NCPPB 790 Strain B03167 Strain NCPPB 339 Strain NCPPB 909

Figure S1. Conventional PCR and BIO-PCR assays targeting fliC gene encoding the flagella subunit. The detection sensitivity of the assays was illustrated for the 5 strains of Ralstonia solanacearum including biovar 2 (race 3) at a high level after enrichment in the BIO-PCR (A) than that in the conventional PCR (B). Dilution series of potato sap sample spiked with each strain were listed in Table 1. M: 100 bp ladder; P: NCPPB 325 DNA serves as a positive control; N: Sterile distilled water serves as a negative control; HN: Healthy potato sap without pathogen serves as a negative control for DNA extraction.

M P N HN M M P N HN M M P N HN M M P N HN M M P N HN M Strain CEPD 7Strain NCPPB 790 Strain B03167 Strain NCPPB 339 Strain NCPPB 909 Fig. S2. Multiplex PCR for phylotyping Ralstonia solanacearum strains. Dilution series of potato sap sample spiked with each strain were listed in Table 1. M: 100 bp ladder; P: NCPPB 325 DNA serves as a positive control; N: Sterile distilled water serves as a negative control; HN: Healthy potato sap without pathogen serves as a negative control for DNA extraction.

M N P HP M Fig. S3. Taqman real-time PCR assay illustrating the detection sensitivity of Ralstonia solanacearum biovar 2 (race 3) (A, B and C) while biovars 1 (D) and 3 (E) are negative. Dilution series of potato sap smaple spiked with each strain were listed in Table 1. M: Hyper Ladder V; N: Sterile distilled water as a negative control; P: NCPPB 909 DNA as a positive control; HN: Healthy potato sap sample without pathogen as a negative control for DNA extraction. D: Strain CEPD 7 E: Strain NCPPB 790 A: Strain B03167 B: Strain NCPPB 339 C: Strain NCPPB 909