 Facilities Open House Functional Genomics Facility Molishree Joshi, Ph.D. 6/1/2015 Contact Information: Phone: Location: B140, Porter Bioscience Building

What is Functional Genomics?  Functional genomics is a field of molecular biology that enables exploration of genes, protein functions and interactions on a global scale.  Functional genomics focuses on the dynamic aspects such as gene transcription, translation, and protein– protein interactions, as opposed to the static aspects of the genomic information such as DNA sequence or structures  It is characterized by high-throughput or large-scale experimental methodologies combined with statistical or computational analysis of the results Tools for Functional Genomics At the DNA level DNA sequencing Genetic interaction mapping The ENCODE project At the RNA level: transcriptome profiling SAGE (Serial analysis of gene expression) Microarrays RNAseq GROseq At the protein level: protein–protein interactions Yeast two-hybrid system Affinity purification and mass spectrometry Loss-of-function / Gain-of-function techniques Mutagenesis RNAi (RNA interference)...shRNA ✔ cDNA / ORF ✔ Genome Editing ZFN TALEN CRISPR ✔ Functional annotations for genes Functional genomics and bioinformatics

Products and Services shRNA Glycerol Stock Plasmid Lentiviral Suspension Premade Pathway- specific Panels Pre-made Genome- wide shRNA libraries CUSTOM shRNA Pools ORF Glycerol Stock Plasmid Lentivirus Suspension Cell Lines Pre-made Cell lines with TRC1/1.5 shRNA Library HCT116 RKO H460 A549 SJSA Custom creation of cell lines with shRNA library Technical Support CRISPR CRISPR- Cas9 Genome- wide Libraries SAM Library CRISPR Vectors Custom sgRNA Cloning Optimized protocols: Lentiviral suspension, Illumina library prep, sgRNA cloning, etc.

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Acknowledgement  Molecular Cellular and Development Biology, CU Boulder  Bio frontiers Institute  Espinosa Lab  NCI Cancer Center Support Grant (P30CA046934)  Howard Hughes Medial Institute