Today House Keeping –Final report (rubric, due date) –Insert, (your) primer walking. 15min.ppt fungal sequences, clones, NGS,CARC Edit, assemble Parasite.

Slides:



Advertisements
Similar presentations
You start with a biologically relevant protein from a pathogen (Bacterium, virus, parasite…)
Advertisements

Polymerase Chain Reaction - PCR The photocopier of molecular biology.
Expression of the lacZ Gene from the ibpB Heat Shock Protein Ebee Hornbeck & Sheena Donald.
Transposable Mutagenesis for an E. coli Methionine or Histidine Synthesis Gene Jon Kissel Mary Maschek.
Synthetic Biology Crash Course DAY ONE. Pre-Assessment 1:00-1:10.
DNA Sequencing Kabi R. Neupane, Ph.D. Leeward Community College ABE Workshop 2006.
Analysis of Gene Expression - Overview -
What are the three steps in PCR?. Denaturation Hybridization of Primer DNA replication.
Restriction Mapping of Plasmid DNA. Restriction Maps Restriction enzymes can be used to construct maps of plasmid DNA Restriction enzymes can be used.
Biotechnology Overview
TOPICS IN (NANO) BIOTECHNOLOGY Lecture 7 5th May, 2006 PhD Course.
Genetic Engineering. Tools of Molecular Biology DNA Extraction Cutting DNA Restriction Enzymes Recognize certain sequences of DNA and cut the hydrogen.
Analysis of Hot Spring Microbial Mat
Warm Up Create a Vocabulary 4 Square As we identify the terms in our lesson: 1.Define the term 2. Give an example 3. Draw a picture to help you remember.
Unit 8 test Biotech study guide.
Revision – Concept map.
AP Biology DNA Study Guide. Chapter 16 Molecular Basis of Heredity The structure of DNA The major steps to replication The difference between replication,
DNA Technology Chapter 11. Genetic Technology- Terms to Know Genetic engineering- Genetic engineering- Recombinant DNA- DNA made from 2 or more organisms.
CS 461b/661b: Bioinformatics Tools and Applications Software Algorithm Mathematical Models Biology Experiments and Data.
Do Now: 3/16 Period 4: Science & Computers 3 rd period today – get right to work! Period 2: Radiation levels in Tokyo are 20 times above background levels,
Biotechnology Practice Test. Question #1 An organism’s chromosomes are part of its a) plasmid b) recombinant DNA c) genome d) enzymes.
INTRODUCING…. THE APPLORANGE Finally and orange with an edible peel.
Today Housekeeping Lab Math Preparing reagents Field trip Topics
Lab Methods in Molecular Biology Bio FALL 2015 M/F Molecular biology of parasites, snails, plants and fungi You, Adema, Natvig, Hutchinson.
Today House Keeping –(field trip, ppts) Update on research progress –(what have we done) Clean up PCR reactions Direct sequencing.
AP Biology Biotech Tools Review AP Biology Biotech Tools Review  Recombinant DNA / Cloning gene  restriction enzyme, plasmids,
Molecular Biology II Lecture 1 OrR. Restriction Endonuclease (sticky end)
Today HK DNA samples (gel and spec) PCR background PCR targets – snail 16S and CO1 – parasite rDNA 18S and 28S Compose PCR reactions AmpliTaq Gold (ABI)
Today  Housekeeping  Gel electrophoresis  (Complete DNA extractions)  Prepare an 1% (w/v) agarose gel  Run DNA on the gel (45 min)  Spectrophotometry.
Today Do you have PCR amplicons? Run gel Background DNA and our PCRs Interpretation of PCR results What to do next?
Today House Keeping –RT-PCR result –Final report (manuscript style) C-value paradox.ppt Phylogeny, COI: snail species ID-ed fungal sequences.
Today House Keeping –CARC? RNA extraction, DNAse treatment Quality Control: Bioanalyzer Stay on schedule, breaks are for Background Fungal amplicons Exosap,
Today Extension product purification (direct sequencing) House Keeping
August 20, 2007 BDGP modENCODE Data Production. BDGP Data Production Project Goals 21,000 RACE experiments 6,000 cDNA’s from directed screening and full.
Today House Keeping (schedule/additional sequencing) 15.ppt Conrad PCR; T-gradient PCR redo parasite P5 (four targets) PCR with 2 snails from Sevilleta.
Variable positions in U1A6 snRNA Qie Kuang Qie Kuang
Molecular Cloning.
DNA Technology & Genomics
Today House Keeping (midterm/additional sequencing) Updates: T-gradient PCR, Sevilleta snails and parasite P5 Cloning TOPO TA 15.ppt Ian/ESTs Direct sequencing.
Today Blue/White screening Surface sterilization Sequence editing.
Today House Keeping (schedule,HW) Sequencing extension product precipitation (constructs) Editing, BLAST 30min PPT Dual activities –Editing, BLAST, direct.
Today House Keeping –CARC –MIDTERMs Exosap, sequencing fungal amplicons Ppt presentation Primer design.
Today House Keeping (talks, sequencing) Follow-up on cloning
Today HOUSEKEEPING, background, presentations DNA extraction from snails DNA extraction from parasites (cercariae) Start protocols (timing!) Background.
Today House Keeping (talks, sequencing) Follow-up on insert check Sequencing Plant DNA extractions 15 minute PPT Sequencher plus BLAST.
Today House Keeping Plasmid extraction, EcoRI digest PCR plants 15.ppt Bianca/microarrays (gels)
Today House Keeping –CARC –Primer order –Final report (manuscript style) Start RT(-PCR), PCR next class Precipitate fungal sequencing reactions RNA Quality.
Today House Keeping (schedule, PCRs 16S, COI) 30’.ppt talk Transfer of colonies Visit MBF 14.00h Complete transfer Intro editing chromatograms Edit and.
AIM: Genetic Engineering: changing the DNA of living organisms. 1. Inserting genes into other organisms 2. Selective Breeding 3. Cloning.
Vocab review Unit 8 - biotechnology. 1. Organism that has acquired genetic material by artificial means.
Topic Cloning and analyzing oxalate degrading enzymes to see if they dissolve kidney stones with Dr. VanWert.
Molecular Cloning. Definitions   Cloning :   Obtaining a piece of DNA from its original source (Genome) and introducing it in a DNA vector   Sub-cloning:
Today House Keeping –Final report (due date) Sequence submissions fungal sequences, clones, NGS,CARC.
Today House Keeping –CARC –Course evaluation on-line soon, –Primer order –Final report (manuscript style) Continue RT-PCR, PCR Phylogeny, COI 15min.ppt.
DNA Isolation. Nucleic Acid Structure & Function DNA & RNA are composed of Nucleotides A nucleotide consists of three covalently-linked parts: –A nitrogen.
Aim: To develop a new one-step RT-PCR assay to detect H1N1 by designing new primer to target NP gene Experimental approach: -nasopharyngeal swabs from.
- making changes to the DNA code of a living organism.
Restriction digestion DNA concentration Gel electrophoresis
Emily Eder HC70AL - Spring 2005
Biotechnology Practice Test
Figure 20.0 DNA sequencers DNA Technology.
اجابة السؤال الاول.
Reads aligned into contigs
DNA sequencing technology
Restriction Enzymes and Plasmid Mapping
Biotech Tools Review
Strategy for F1 mutation carrier screening and identification of their mutations. Strategy for F1 mutation carrier screening and identification of their.
Bioinformatics Lecture By: Ms AQSAD RASHDA
Week 1: Tutorial Outline
Practical Contents DNA Extraction Gel Electrophoresis
Presentation transcript:

Today House Keeping –Final report (rubric, due date) –Insert, (your) primer walking. 15min.ppt fungal sequences, clones, NGS,CARC Edit, assemble Parasite Physa 5 (PP5)28S

Final Reports Topics – Snail/parasite identification (RT-PCR) – Fungal metagenomics Format: manuscript for submission – Format: Research note, Journal of Parasitology

REPORTS in PDF format Due 15.45h, Wednesday December 9th Key for final report Correct format (order/spacing/legends/etc.) Correct methods Basic results Appropriate references Discussion (analysis) 30% 20% 15% 25% of final grade for 446L (100/400) 20% of final grade for 546L (100/500)

clone3seq.zip

PARASITES AND SNAIL BIOLOGY “identity, possibilities” phylogenetics “intentions” transcriptomics PCR rDNA/mito Bioanalyzer DNA-free, direct sequencing gel electrophoresis nanodrop spec Sequence ID (BLAST) editing Phylogenetics electrophoresis RT-PCR gel CTAB Trizol TA cloning, B/W screening M13 sequencing Primer design, walking Qiagen plasmid extraction Restriction digests DNA RNA GenBank submission + Sevilleta snails

15min.ppt

fungal sequences, clones, NGS,CARC

Edit, assemble Parasite Physa 5 (PP5)28S

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.