Fig. S1: Fingerprinting of three BAC clones from different accessions of wild rice species with the AA genome constitution. The BAC DNAs were completely.

Slides:

Advertisements

Similar presentations

Biotechnology. Comparative genomics also has been used to identify recently mobilized transposons in genetically diverse humans. For example, over 600.

Advertisements

Mouse Genome Sequencing

-The methods section of the course covers chapters 21 and 22, not chapters 20 and 21 -Paper discussion on Tuesday - assignment due at the start of class.

Recombinant DNA Technology and Genomics A.Overview: B.Creating a DNA Library C.Recover the clone of interest D.Analyzing/characterizing the DNA - create.

Linkage and Mapping. Figure 4-8 For linked genes, recombinant frequencies are less than 50 percent.

Figure S1. RACE mapped transcription starts and polyA signals of Ogre CL5 and Ogre CL5del and putative splice site of Ogre CL5 and Ogre CL5del in Silene.

Supplementary Fig 1. Multiple sequence alignment of nucleotide sequences from amplified region of different Oryza sativa lines and wild species of rice.

Supplemental Materials of Two conserved oligosaccharyltransferase catalytic subunits required for N-glycosylation exist in Spartina alterniflora Luyi Jiang.

2470 bp 1891 bp WT bp 2314 bp A B Fig. S1. Verification with PCR amplification of the.

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Lisa Edelmann, Raj K. Pandita, Bernice E. Morrow

Volume 6, Issue 4, Pages (October 2000)

Alternative Splicing of a Novel Glycophorin Allele GPHe(GL) Generates Two Protein Isoforms in the Human Erythrocyte Membrane by Cheng-Han Huang, Olga O.

Mark M Metzstein, H.Robert Horvitz Molecular Cell

RHD gene deletion occurred in the Rhesus box

Jayne Griffiths, Marco Catoni, Mayumi Iwasaki, Jerzy Paszkowski

by Cheng-Han Huang, Ying Chen, Marion E. Reid, and Christine Seidl

The Mre11 Complex Is Required for Repair of Hairpin-Capped Double-Strand Breaks and Prevention of Chromosome Rearrangements Kirill S. Lobachev, Dmitry.

Isothermal Multiple Displacement Amplification

by Jean-Michel Cayuela, Betty Gardie, and François Sigaux

Christoph Heiner Westphal, Philip Leder Current Biology

Volume 7, Issue 4, Pages (October 1997)

Volume 10, Issue 1, Pages (July 2002)

A Member of a Gene Family on Xp22

Hou-Sung Jung, Gregory J. Tsongalis, Joel A. Lefferts

HKAP1.6 and hKAP1.7, Two Novel Human High Sulfur Keratin-Associated Proteins are Expressed in the Hair Follicle Cortex Yutaka Shimomura, Noriaki Aoki,

A Novel Alu-Like Element Rearranged in the Dystrophin Gene Causes a Splicing Mutation in a Family with X-Linked Dilated Cardiomyopathy Alessandra Ferlini,

Improved system for helper-dependent adenoviral vector production

Genomic Rearrangements Resulting in PLP1 Deletion Occur by Nonhomologous End Joining and Cause Different Dysmyelinating Phenotypes in Males and Females

Volume 10, Issue 11, Pages (November 2017)

Molecular Characterization and Gene Content of Breakpoint Boundaries in Patients with Neurofibromatosis Type 1 with 17q11.2 Microdeletions Dieter E.

Figure 1. Generation of the S250F Aadc mutant mice

Developmentally Programmed Rearrangement of T Cell Receptor Vγ Genes Is Controlled by Sequences Immediately Upstream of the Vγ Genes Jeanne E Baker,

Investigation of the human stem cell factor KIT ligand gene, KITLG, in women with 46,XX spontaneous premature ovarian failure Emily S. Hui, B.A., Ekemini.

Human Epidermal Differentiation Complex in a Single 2

Volume 154, Issue 6, Pages (September 2013)

Analysis of an exon 1 polymorphism of the B2 bradykinin receptor gene and its transcript in normal subjects and patients with C1 inhibitor deficiency

Origin of Immunoglobulin Isotype Switching

Size Polymorphisms in the Human Ultrahigh Sulfur Hair Keratin-Associated Protein 4, KAP4, Gene Family Naoyuki Kariya, Yutaka Shimomura, Masaaki Ito

Dissemination of multidrug-resistant, class 1 integron-carrying Acinetobacter baumannii isolates in Taiwan L.-Y. Huang, T.-L. Chen, P.-L. Lu, C.-A. Tsai,

Supplemental Figure 3 A B C T-DNA 1 2 RGLG1 2329bp 3 T-DNA 1 2 RGLG2

Volume 9, Issue 6, Pages (December 1998)

Structure of the GM2A Gene: Identification of an Exon 2 Nonsense Mutation and a Naturally Occurring Transcript with an In-Frame Deletion of Exon 2 Biao.

Volume 10, Issue 8, Pages (April 2000)

A Human Homologue of the Drosophila melanogaster diaphanous Gene Is Disrupted in a Patient with Premature Ovarian Failure: Evidence for Conserved Function.

Volume 18, Issue 2, Pages (April 2005)

Comprehensive Mutation Analysis of the CYP21A2 Gene

A Multi-Exonic BRCA1 Deletion Identified in Multiple Families through Single Nucleotide Polymorphism Haplotype Pair Analysis and Gene Amplification with.

Volume 84, Issue 3, Pages (February 1996)

Volume 17, Issue 1, Pages (July 2002)

A Mutation in the Variable Repeat Region of the Aggrecan Gene (AGC1) Causes a Form of Spondyloepiphyseal Dysplasia Associated with Severe, Premature.

Sex-Linked period Genes in the Silkmoth, Antheraea pernyi

The mlenapts RNA Helicase Mutation in Drosophila Results in a Splicing Catastrophe of the para Na+ Channel Transcript in a Region of RNA Editing Robert.

Volume 58, Issue 2, Pages (August 2000)

APOE Gene Targeting (A) Schematic representation of the endogenous APOE locus, the gene targeting vector and the targeted APOE locus. The exons of the.

Material for Quiz 5 from Chapter 8

Multiple Developmental Stage–Specific Enhancers Regulate CD8 Expression in Developing Thymocytes and in Thymus-Independent T Cells Wilfried Ellmeier,

B. Bakhshi, M.R. Pourshafie, F. Navabakbar, A. Tavakoli

Assessing the Functional Characteristics of Synonymous and Nonsynonymous Mutation Candidates by Use of Large DNA Constructs A.M. Eeds, D. Mortlock, R.

Volume 16, Issue 4, Pages (April 2002)

Anthony M. Raizis, Martin M. Ferguson, David T. Nicholls, Derek W

Bart A. Jessen, Marjorie A. Phillips, Robert H. Rice

Expression of multiple forms of MEL1 gene products.

MicroRNA Binding Sites in Arabidopsis Class III HD-ZIP mRNAs Are Required for Methylation of the Template Chromosome Ning Bao, Khar-Wai Lye, M.Kathryn.

Volume 21, Issue 23, Pages (December 2011)

Structure of the IFL1 Gene and the Nature of the Mutations in the ifl1 Alleles.(A) A schematic representation of the exon and intron organization of the.

Mutation of the Ca2+ Channel β Subunit Gene Cchb4 Is Associated with Ataxia and Seizures in the Lethargic (lh) Mouse Daniel L Burgess, Julie M Jones,

Kit-Sing Au, Adelaide A. Hebert, E. Steve Roach, Hope Northrup

Southern blot demonstrating allelic heterozygosity of the vspC5 gene as well as members of the family of genes related to vspC5. Southern blot demonstrating.

Volume 1, Issue 5, Pages (September 2008)

Presentation transcript:

Fig. S1: Fingerprinting of three BAC clones from different accessions of wild rice species with the AA genome constitution. The BAC DNAs were completely digested with the restriction enzyme HindIII and resolved on a 0.8% agarose gel. HindIII- fingerpritings of the BACs were revealed by Southern hybridization following the instructions of the ECL labeling and detection kit (Cat. No. RPN3000, GE, USA). Three Southern hybridizations were done using the whole BAC DNA as the probes: A. OR_BBa0100B19 (Lane 1); B. OR_CBa0092G16 (Lane 2); and C. OR_CBa0094P06 (Lane3). The probe used in the Southern hybridization was marked in asterisk. The extra HindIII-digested band in OR_BBa0100B19 is marked with an arrow to show that OR_BBa0100B19 is the largest clone. 1* * * ABC

OM_Ba0177F21 OM_Ba0178G06 OM_Ba0049B19 OM_Ba0180N01 OM_Ba0165G09 OM_Ba0024C01 NIPPK NBS-LRR cluster Fig. S2: BAC contig covering the Pi2/9 locus in the CC genome of O. minuta. The BAC clones are indicated in rectangles with their names above. Three BACs selected for sequencing are indicated in filled rectangles. The physical relationship between OM_Ba0177F21 and OM_Ba0165G09 in shadow was confirmed by PCR validation and sequencing of the PCR products. Both flanking genes [nitrate inducted protein (NIP) and protein kinase (PK)] are shown in filled boxes. The central NBS-LRR gene cluster is shown in an oval. The figure is not drawn in scale.

Nbs12-OM- CC Nbs4-OM-CC Nbs6-OM-CC VG 4 VG 5 VG 7 VG 1 VG 2 VG 6 VG 3 VG 8 Fig. S3: Cladistic analysis of the Pi2/9 gene family members in the Oryza genus. The cladistic tree was constructed based on the sequences of the entire coding sequence (CDS) of the Pi2/9 gene family members in the four wild rice species as well as those in cultivated rice lines. Clustalx and MEGA 4 programs were used for multiple sequence alignment and for tree viewer, respectively. Three genes (Nbs12-OM-CC, Nbs4-OM- CC, and Nbs6-OM-CC) containing large unsequenced gaps are shadowed in their respective clades. The vertical groups (VGs) are shown to the right of their respective clades.

Fig. S4: Genomic expansion of the Pi2/9 locus by insertion of transposable elements in the BB subgenome of O. minuta. This figure was modified from Fig. 1 mainly by adding the information of the transposable elements (TEs) in the BB subgenome of O. minuta (A) and O. puncata (B). The TEs are indicated in grey rectangles, and their names are listed above. Detailed information for all the TEs is in Table 3. The shadows linking both genomes indicate the regions that are highly similar to each other, i.e., with ≥90% sequence identity in nucleotides on average. TE1TE3TE4 and TE5TE6 TE7 TE8 TE1TE2TE3 A B TE2 10 kb

NBS LRR Pi9 Nbs2-OS-IR31917 Fig. S5: Schematic of the amplification of Nbs2-OS-IR The exons are indicated in red rectangles. The dashed lines in Nbs2-OS-IR31917 indicate the unamplified region. The shadows indicate the amplified and sequenced regions in Nbs2-OS-IR31917.