Mekanisme Kerja Hormon dr. Susila Sastri M.Biomed Biokimia FK-Unand
Hormone
Struktur Kimia Hormon 1.Polipeptida Insulin Glucagon 2.Derivat asam amino (amin) Epineprin Serotonin Tyroxin 3. Steroid (derivat kolesterol) Aldosteron Cortisol Retinoic acid 4. Eicosanoid (polyunsaturated acid, (20atom C) Prostadglandin E Thromboxane A2
Simple Intracellular & Extracelluler Signaling
Hormon Action
Hypophise Anterior
Neurohormone
GAS MOLECULE Cell Communication
Second messenger: Small molecules synthesized in cells in response to an external signal are the second messengers, which are responsible for intracellular signal transduction: Ca 2+, DG, Cer, IP 3, cAMP, cGMP
Receptor Receptors: specific membrane proteins, which are able to recognize and bind to corresponding ligand molecules, become activated, and transduce signal to next signaling molecules: A. Membran B. Intra Sel Glycoprotein or Lipoprotein
Hypothalamus Anterior pituitary Posterior pituitary Thyrotropin ACTH Somatotropin LH FSH Prolactin Vasopressin Oxytocin Thyroid Adrenal Cortex Adrenal Medulla PancreasOvary Testis Muscles liver Tissues Liver, muscles Estradiol Testosterone Insulin, glucagon, somatostatin T3 Cortisol aldosterone Mammary glands Reproductive organs Epinephrine Releasing hormones Nervous Kelenjer-Hormon-Target Sel
1. Membrane receptors
Signal transduction mediated by membrane receptor cAMP dependent-protein kinase A pathway cGMP dependent PKG pathway Ca 2+ dependent PK pathway Tyrosine protein Kinase pathway NF-κB pathway TGF- βpathway
Membrane Receptor
Cyclic AMP
Mekanisme Signal Tranduksi
Glucagon Cyclic AMP Cyclic GMP Ca 2+ Diacylgycerol Protein substrates PK-APK-G CalmodulinPK-C Protein Ser/Thr kinases Protein substrates Multifunctional kinases Other phospholipases Tyrosine kinase IP 3 GGGG Insulin T-cell Activation Nitric oxide G protein End result is phosphorylation of one or more proteins Hormon-2 nd Massengger- Enzim
Inositol triphosphate System PLC; phospholipase C PIP2: phosphoinositol DAG:diacylgliserol IP3:inositol triphosphat PKC: phosphokinase C
Intracellular receptor (DNA transcription regulated receptor) Steroid Hormone, VD 3, Thyroxine Cytosolic R: glycocorticosteroid H Nuclear R: thyroxine, estrogen, androgen, progesterone
Hormon - Receptor highly specificity highly affinity saturation reversible binding special function model
Control of receptor activity Phosphorylation or dephosphorylation of R Phospholipid of membrane Enzyme catalyzed hydrolysis G protein regulation
Thyroid Mechanism of Action
Adrenaline Mechanism of action
ACTH Mechanism of Action
Aldosteron Mechanism of Action
Estrogen Mechanism of Action
Androgen Mechanism of Action
Insulin Receptor
Insulin Action
Glucagon
GH/ STH