DNA – November 17, 2010 1.What should this class sound and look like during a lab activity? (Describe what Ms. Moore would see if she came in here) 2.What.

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DNA – November 17, What should this class sound and look like during a lab activity? (Describe what Ms. Moore would see if she came in here) 2.What happens if you do not follow the guidelines of the safety contract?

Introduction to Microscopes Ms. Klinkhachorn November 17, 2010 Biology

On Your Lab Handout… Read the section titled “History and Importance of Microscopes”. Answer the questions that follow.

How to Properly Carry a Microscope You will be graded on this! WALK slowly. Hold the microscope at 2 points – One hand on the arm of the microscope – One hand underneath the base

How to Properly Carry a Microscope Base Arm

Identify Microscope Parts and What They Do CAREFULLY go over the microscope and identify the parts. You may move the parts GENTLY in order to figure out what the function of each part is. Fill in the table below with this information.

Identify the Parts

LetterName Function AObjective Lens Magnifies, Can Rotate to Increase/Decrease Magnification BStage Clips Keep slide in place CDiaphragm Adjust the amount of light that goes through your object of study DLight Source Provides the light needed to see EOcular Lens / Eye Piece Magnifies, Part that you look through

LetterName Function FArm Supports the lenses, used to carry the microscope GStage Holds the slide, moves up and down to help focus HCourse Adjustment Knob Moves the stage up and down to focus the image (low power) IFine Adjustment Knob Helps focus on higher powers JBase Supports the entire microscope, used to carry the microscope

How to Store a Microscope Remove any slides from the microscope Move the objective lens to the lowest power. Move the stage as far down as it can go.

Calculating Magnification To calculate the total magnification using a compound light microscope, you must always multiply the power of the ocular lens by the power of the objective lens. Total Magnification = Ocular Lens Power x Objective Lens Power

Sample Problem #1 Jared’s microscope has an ocular lens power of 20. He sets his objective lens power to 10. What will be the total magnification of the object he is observing? Ocular lens = 20 Objective lens = 10 Total Magnification = 20 x 10 = 200

Sample Problem #2 Shelley’s ocular lens has a power of 50. She is looking at a cell under an objective lens of 20. What will be the total magnification of the object she is looking at?

Sample Problem #2 Shelley’s ocular lens has a power of 50. She is looking at a cell under an objective lens of 20. What will be the total magnification of the object she is looking at? Ocular = 50, Objective = 20 Total Magnification = 50 x 20 = 1000