Sources of Bacterial Species with Antibiotic Activity: Soil & Rotten Wood.

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Presentation transcript:

Sources of Bacterial Species with Antibiotic Activity: Soil & Rotten Wood

Collect rotten wood aseptically

Culture bacteria from rotten wood

Streak bacterium for Isolation

Test Isolates for inhibition of pathogens

Gram stain isolate

Extract DNA from Isolates

PCR 16s rRNA gene

Run Gel to test for ample DNA

Send PCR product for sequencing >S NWC-2-CP1 NNNNNNNNNNNNNNNGCGGNTGGNTCCNAAAAGGNTACCCCACCGACTTCGGGTGTTACAAACTCTC GTGGTGTGNNNNNNNNTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT AGCGATTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAACGGTTTTATGAGATTAGCT CCACCTCGCGGTCTTGCAGCTCTTTGTACCGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCAT GATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTTAATGA TGGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGAC AACCATGCACCACCTGTCACTCTGCTCCCGAAGGAGAAGCCCTATCTCTAGGGTTTTCAGAGGATGTCAAG ACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATT CCTTTGAGTTTCAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAACTTCAGCACTAAAGG GCGGAAACCCTCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTC CCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAAAGTCGCCTTCGCCACTGGTGTTCCTCCATATCT CTACGCATTTCACCGCTACACATGGAANTCCACTTTCCTCTTCTGCACTCAAGTCTCCCAGTTTCCAATGACC CTCCACGGTTGAGCCGTGGGCTTTCACATCAGACTTAAGAAACCACCTGCGCGCGCTTTACGCCCAATAAT TCCNNANNACGCTTGCNANCTACGTATTACNGCGNCTGCTGGCNCGTAGTTAGCNNNNGNTTTCTGGNT AGGTACCGNCANNNNCCAGNTNNNTNANNAGNNNTNGTNNTNCNNANNNANNGANTTNACNACCC NNANNCTNNNTCACTCNNNNNNNGTTGCN

Use sequence for BLAST search

Search journals

You’ve found a new antibiotic!

Was the difference significant? Tundra SoilRotting Wood Total Tested Isolates with Activity 54 81

Two variable Z test for population proportions

p value =

Cupressaceae vs alternative tree families

Future Efforts

Wyoming INBRE Northwest College Mentors: Dr. A. Childs, Dr. S. Harbron, and Dr. E. Kimble. Sue Norris (Statistics) Fellow Students