Utilizing Skein Eggs as an Alternative Egg Source For Bloater Reintroduction into Lake Ontario K. J. NASH, M. A. CHALUPNICKI, J. H. JOHNSON USGS Great.

Slides:



Advertisements
Similar presentations
Colville Confederated Tribes and the Okanagan Nation Fisheries Commission.
Advertisements

Spatial scales of homing and the efficacy of hatchery supplementation of wild populations Northwest Fisheries Science Center National Marine Fisheries.
Introduction of Aquaculture Into High Schools 45 New York Schools 11 Northeast Schools 2 Correctional Institutes.
Introduction This Hatching Picture Tutorial is a product of the Catfish Hatchery of Abaflix Emerald Investment Ltd, Plot 6, Sanni-Luba Street, G.R.A, Ijebu-Ode.
BioEYES in the Classroom. BioEYES: Day 1 The students will be divided into groups and will be able to choose a male and female fish. The students will.
Lake Sturgeon (Acipenser fulvescens)
Will Coker. us Sciaenops Ocellatus Wide distribution spreading from the Western Atlantic to Mexico and S. America Found in sandy or muddy coastal waters.
  Multiple years of sampling to mark and recapture individuals completed between 2006 and 2008   Despite significant effort, population estimates were.
Waterfront Recreation (photo of big boat going by) Module 6:1.
THE LARVAL HATCH FECUNDITY OF A STOCK OF THE GIANT FRESH WATER PRAWN Macrobrachium rosenbergii By. George B. Brooks, Jr. Ph.D. Environmental Coordinator/Aquatic.
Life history Broodstock –Spawning and care –Fertilization methods Egg development and hatching –Stages of development –Methods of incubation Fry rearing.
The Rusty Crayfish By: Vlad Spirkov, Michael Tsimidis & Matthew Bondi Ecology STSE Changes in Stability of Ecosystems.
Lobsters in Northumberland Jessica Duffill Telsnig (McLoughney, 2013)
Recommendations for a Statewide Water Plan By: Ewan Hadgraft Alabama Rivers Alliance Birmingham-Southern College.
TECHNOLOGY FOR ARTIFICIAL SPAWNING OF STIZOSTEDION LUCIOPERCA SPECIES – PIKEPERCH TECHNOLOGY DISPLAY TECHNOLOGY DISPLAY Object Object The elaborated technology.
Propagating and Selling Fish!
Peter Sigmann Little Sturgeon Area Property Owners Association Tracking Phosphate in Little Sturgeon Bay Location The Bay of Little Sturgeon is located.
Characteristics of Channel Darter Habitat in the Winooski River, Vermont Douglas E. Facey and Shannon M. O ‘Brien Department of Biology Saint Michael’s.
Aquaculture of the Bluefin Tuna. Taxonomy Genus Thunnus Species: Maccoyii, Orientalis, Thynnus.
Spokane Tribal Hatchery Sherman Creek Hatchery Lake Roosevelt Volunteer Net Pens Monitoring by Lake Roosevelt Fisheries.
Kalispel Tribe Resident Fish Program Hatchery - Wayne Gould Habitat- Michele Andersen.
Importance of Protecting Lake Trout. 250,000 lakes in Ontario 1% of these contain lake trout Central/eastern Ontario has >1/3 of lakes Provincial responsibility.
Hair re-growth study Dereje ( Dre ) Yalew /MD,IMG/ Observational Case Study Hair Re-Growth Program using an Electro Stimulator.
U.S. Department of the Interior U.S. Geological Survey USGS Great Lakes Science Center Great Lakes Research.
EPA’s Work Related to P2 and the Great Lakes Great Lakes Regional Pollution Prevention Round Table Summer Conference August 2005.
The Sea Lamprey By: Veena Kanumalla. What is it? Petromyzon marinus Scientific Classification Kingdom: Animalia Phylum: Chordata Class: Cephalaspidomorphi.
Lake Huron Initiative: A Work in Process Presented by: Jim Bredin - Michigan Office of the Great Lakes.
Lake Superior Benthic Fish Community Structure By Michael H. Hoff U.S. Geological Survey Great Lakes Science Center.
A Multi-Agency Effort to Address Declines in Yellow Perch Abundance in Lake Michigan David Clapp Michigan Department of Natural Resources and Yellow Perch.
Aquatic animal diseases, Health care & Management © TANUVAS, 2011 Visit to organized hatchery Contents Components of a fish hatchery Brood fish ponds Breeding.
Fishery Biology. Fisheries Management n Provide people with a sustained, high, and ever-increasing benefit from their use of aquatic resources n Problems.
Introduction Oithona similis is the most abundant copepod in the Gulf of Alaska, and is a dominant in many ecosystems from the poles to the sub-tropics.
Bellevue Planning Commission November 4, Conservation of Lake Sammamish Kokanee An Update on Current Science A Briefing for the City of Bellevue.
Shrimp/Prawn culture Penaied shrimp –3 common species (early on) 1.White, pink, and brown shrimp 2.Asian species (Penaeus monodon) – most widely cultured.
The U.S. Food and Drug Administration (FDA) has classified hydrogen peroxide as a low regulatory priority drug when used at concentrations of up to 500.
Adjusting N:P ratios in liquid dairy manure through nitrification and chemical phosphorus removal to match crop fertilizer requirements Background Nutrient.
KVRI KR Burbot Subcommittee Meeting October 7, 2008 Nathan R. Jensen University of Idaho\Fish and Wildlife Department UI.
Walleye Status in Lake Superior Stephen T. Schram Wisconsin Department of Natural Resources.
Feeding Strategies of Gelatinous Zooplankton Collected at Wallops Island Jennifer L. Cole and Dr. Jessica Nolan Department of Biological Sciences, York.
White Sturgeon of the Nechako and Stuart Watersheds; A Conservation Crisis Presentation by: Christina Ciesielski Presentation by: Christina Ciesielski.
Lower Snake River Comp Plan M & E Program SPY’s thoughts based on 3 weeks.
Unit VIII Post stocking management. INTRODUCTION This phase includes the activities to be undertaken from stocking of fingerlings up to the final harvesting.
Preliminary Data on Euphausiid Distribution and Growth in the Northern Gulf of Alaska. A.I. Pinchuk, R.R. Hopcroft, K.O. Coyle Institute of Marine Science,
The Reinvestigation of Ovule Development Through Megagametogenesis of the Rapid Cycling Brassica rapa L. and the Quantification of Various Stages William.
Overview of the Alligator Gar Conservation Management Plan for Alabama Dave Armstrong Alabama Division of Wildlife & Freshwater Fisheries Spanish Fort,
John Lake – Marine Biologist RIDFW-Marine Fisheries Section 3 Ft. Wetherill Road Jamestown, RI Young-of-the-Year Survey in RI.
Lake Sammamish Kokanee: Bringing Them Back from the Brink A Presentation for the Rotary Club of Issaquah David St. John – Government Relations Administrator.
Assessing Diadromous Fish Restoration in the Kennebec and Androscoggin Rivers Claire Enterline 1, Gail Wippelhauser 1, Nate Gray 1, Paul Christman 1, Karen.
Supplementation using steelhead fry: performance, interactions with natural steelhead, & effect of enriched hatchery environments Christopher P. Tatara.
An Overview of Lake Herring (Coregonus artedii) propagation and production techniques at the UWSP Northern Aquaculture Demonstration Facility and Red Cliff.
The Effects of Vegetation, Nutrition, and Sex Ratio on the Reproductive Cycle of Fundulus heteroclitus in the Laboratory College of Arts and Sciences,
The effects of progesterone and synthetic derivatives on Fathead Minnow (Pimphales promelas) embryos. JA Stine and DB Huggett, Ph.D. Department of Biological.
BC MoE Burbot Recovery Progress 2007 Kootenai Burbot Conservation Strategy.
Fig.1 – Fecundity of Great Lakes cisco as a function of length. Fig.2 – Fecundity of Great Lakes salmonids as a function of length 1 Eggs measured 1 –
Efficacy and Toxicity of Chloramine T Disinfection on Cisco Fry G. E. MACKEY, M. A. CHALUPNICKI, J. H. JOHNSON USGS Great Lakes Science Center, Tunison.
Initial Assessment of Habitat Use by Stocked Lake Sturgeon in the Genesee River D. E. DITTMAN 1 and E. C. ZOLLWEG 2 1 Tunison Laboratory of Aquatic Science,
This presentation was given by, Manfred Kittel, CDFW, at a meeting of the Statewide Coho Recovery Team, June 26-27, 2013.
Early Detection of Invasive Fishes in Lake Superior Joshua Schloesser and Henry Quinlan U.S. Fish and Wildlife Service Ashland Fish and Wildlife Conservation.
An Overview of Lake Herring (Coregonus artedii) propagation and production techniques at the UWSP Northern Aquaculture Demonstration Facility and Red.
Thiamine Variations in Rainbow Smelt from the Great Lakes
Brown Trout Growth: Growing, Growing, or Gone
Use of Iodine to Disinfect Atlantic Salmon Eggs
DISTRIBUTION OF LAKE STURGEON FOLLOWING 11 YEARS OF STOCKING
Calcein as an Alternative Chemical Marker for Lake Herring
Southern California Green Abalone Restoration Project
Images of the Great Lakes (with captions in “notes” pane)
Team Maggot: Designing a Better Bait Cup
Technical Briefing Northern Shrimp Stock Assessment
Culture of Hybrid Striped Bass In the U. S.
Egg Deposition, Development, and Survival
Presentation transcript:

Utilizing Skein Eggs as an Alternative Egg Source For Bloater Reintroduction into Lake Ontario K. J. NASH, M. A. CHALUPNICKI, J. H. JOHNSON USGS Great Lakes Science Center, Tunison Laboratory of Aquatic Science Cortland, NY N. H. RINGLER SUNY College of Environmental Science and Forestry Syracuse, NY ABSTRACT: Restoration of native fish populations into Lake Ontario is a multi-agency effort within New York State. Populations of bloater (Coregonus hoyi), a deep water cisco, over the past century have become extirpated in Lake Ontario due to overharvesting and competition with invasive species. The reintroduction of bloater as an alternative forage fish to non-endemic species has given rise to new culture methods utilizing the availability of current stable populations in Lake Michigan. Specific efforts for new culture techniques have been made for potential utilization of skein-bound eggs to produce viable offspring. Retrieving skein eggs for the purpose of reintroducing bloater back into Lake Ontario would be beneficial due to the large quantity available. In 2015, we evaluated the growth and survival of spawned ripe and skein-bound bloater eggs. The egg diameter of skein eggs was significantly smaller than the ripe eggs but did not appear to effect survival or development. Overall, the survival of ripe eggs was slightly higher than skein eggs but was not significantly different. In the first two months post-hatch survival was greater for “skein” fish, with “ripe” fish survival becoming slightly higher by the fall. Implementation by management agencies of skein-bound eggs as an alternative egg source may benefit restoration efforts of bloater into Lake Ontario. METHODS: Adult bloater were captured near Two Rivers (Wisconsin ) in Lake Michigan by commercial fishing trawlers in January with the assistance of the USFWS Green Bay Office and Jordan River National Fish Hatchery. The adults were evaluated for readiness to spawn as “ripe” where eggs released freely or “skein-bound” where eggs were not fully detached from the ovary and were physically removed. Eggs were fertilized and kept in separate containers and shipped overnight to TLAS and WLFCS for rearing (Figure 2). Once received, eggs were immersed for 10 minutes in culture water containing 25mg/L polymeric iodine (Argentyne, Argent Chemical Labs, Redmond, Washington) while gently being stirred and then rinsed 3x. To prevent egg adhesiveness, eggs were then immersed for 15 minutes in water containing 66 units of bacterial protease (Protease, Bacillus lichenifarmes, Sigma Chemical Co., St. Louis, Missouri) with occasional gentle stirring to prevent clumping. The eggs were again rinsed 3x and transferred to McDonald jars (Figures 3-4). Egg motion was evaluated and flow rates were measured and recorded daily. Floating, dead, and fungus eggs were siphoned off daily. Eggs were treated with 0.17% formalin for 15 min 2-3 times a week to prevent and control fungus levels. All effluent underwent UV treatment in order to prevent any potential disease spread. Embryogenesis was evaluated and monitored weekly throughout the incubation period using a ( x) Leica digital microscope camera with digital imaging software. Weekly sub-samples of eggs from both ripe and skein adults were evaluated for developmental milestones and overall survival. Evaluation consisted of viewing and enumerating 1 ml of eggs from each sample under a dissecting microscope equipped with the digital microscope camera. Live and dead eggs were counted and developmental progress was noted. Embryonic milestones were defined as 1) Fertilization with cell division, 2) Notochord formation, 3) Body and head formation, 4) Eye up, 5) Heart beat observed, 6) Myomeres/pigmentation completed, 7) Hatch (Figure 5). Once hatch occurred evaluation of ripe versus skein egg success was complete. Hatched fry were transferred to green circular takes where they were allowed to grow. Growth and survival were recorded until fish were stocked in November. Differences in egg diameter between ripe and skein eggs were evaluated at fertilization and eye up using paired t-tests (Statistix 8.0, Tidepool Scientific, Tallahassee, Florida). Paired t-tests were also used to determine if the percent of viable eggs between ripe and skein at each developmental milestone were significantly different. We considered p<0.05 to be significantly different. RESULTS: Bloater skein eggs were significantly smaller than ripe eggs at fertilization (p<0.01, T Stat=3.17, DF=69) and at eye up (p=0.02, T Stat=2.38, DF=42) (Table 1). Although smaller, skein eggs enlarged 3x more than ripe eggs over the course of development (0.043mm v 0.015mm). Through all developmental milestones, ripe eggs had a higher percent survival than skein eggs but were not significantly different (Table 2). Survival after hatch was greater for skein fry initially for the first two months, but leveled off with ripe fry survival becoming slightly higher in the fall. INTRODUCTION: Bloater (Coregonus hoyi) is a native species within the Great Lakes whose populations have been extirpated from Lake Ontario as a result of overharvesting by commercial fishing and competition by non-endemic species including Alewife (Alosa pseudohorengus). Currently, there is a multi-agency and binational initiative between the US Fish and Wildlife Service (USFWS), Ontario Ministry of Natural Resources and Forestry (OMNRF), N.Y. Department of Environmental Conservation (NYSDEC), Great Lakes Fisheries Commission, and U.S. Geological Survey (USGS) to reestablish bloater back into Lake Ontario. The Tunison Lab of Aquatic Science (TLAS) in Cortland, NY and White Lake Fish Culture Station (WLFCS) in Ontario, Canada are developing specific hatchery techniques to rear bloater for re- introduction into Lake Ontario. By utilizing a stable population captured from Lake Michigan, we have developed several techniques for yielding successful bloater within a hatchery setting. We experimented with “skein-bound” eggs; eggs enveloped within ovarian tissue, as a potential alternative egg source to produce viable offspring (Figure 1). Acquiring large quantities of viable eggs from Lake Michigan has been previously problematic due to poor environmental conditions, low catch rates, and low collection rates resulting from the expelling of free flowing (ripe) eggs from stress during capture. The utilization of skein eggs due to the large quantity available may boost bloater restoration efforts. The objective of this study was to highlight the early life ontogenetic milestones of bloater development from skein-bound and ripe eggs through hatch as means to compare their overall survival. CONCLUSIONS: The potential utilization of skein-bound eggs as a viable alternative egg source allows the reintroduction effort to substantially increase the overall quantity of eggs obtained. These results show that although skein-bound eggs are smaller in diameter, the overall survival was not significantly different through all egg developmental milestones. This information enables the reintroduction effort to successfully utilize skein-bound eggs as an additional source for yielding viable offspring. Implementation of these results will allow management agencies the capability of increasing overall bloater restoration efforts and have the potential to achieve targeted egg quantity goals allowing for greater stocking potential into Lake Ontario. Further observation of long term survival including behavioral and physiological deficiencies still needs to be assessed. Figure 2: Bloater collection sight and distributions Figure 1: Skein-bound eggs being removed from bloater females Figure 3: Skein-bound eggs in jar Figure 4: Ripe eggs in jar Figure 5: Developmental milestones of bloater egg development Table 1: Ripe versus skein egg diameter at fertilization and eye up stages of embryogenesis. Ripe eggs contained an overall larger diameter but a drastic increase in diameter between these two developmental milestones occurred with skein-bound eggs. Table 2: Overall survival of ripe versus skein-bound eggs from embryogenesis through stocking. Although ripe eggs had an overall higher survival, no significant difference occurred. Between May-July skein-bound fry experienced greater survival than ripe fry.