Gram-negative coccobacilli and cocci Prof. Dr. Asem Shehabi Faculty of Medicine University of Jordan University of Jordan.

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Gram-negative coccobacilli and cocci Prof. Dr. Asem Shehabi Faculty of Medicine University of Jordan University of Jordan

Haemophilus Group  Gram-negative cocco/large- thin bacilli.. Aerobic.. Requires growth factors ( V- X-factors).. Grow around S. Staphylococcus.. S. Staphylococcus..  Normal Flora.. Human Respt. Tract.. Died Rapidly Outside Body( 30 minutes) Many Haemophilus sp. Virulenc factors: Endotoxin & Capsule.. Virulenc factors: Endotoxin & Capsule..  H. influnzae type b: Most common pathogenic species.. capsulated.. Causing Localized and Invasive Infect.. Sore Throat, Otitis Media, Sinusitis, Conjunctivitis, Brochopneumonia, Septicemia & Meningitis.. Children 6 Months-5 Years.  Lab Diagnosis: Blood, CSF & Others Culture.. Chocolate & blood Medium.. X & V Factors.. Antibiotics.. Hib-Vaccine.. Infants > 2 months old.

Haemophilus colonies growth surrounding Stapholococcus colonies

Bordetella pertussis  Gram-negative coccbacilli.. Aerobic.. Highly Communicable agent.. Droplets Infection..close contact.. Mucosa Respiratory Tract.. Produce Pertussis Toxin.. Whooping Cough/ paroxysmal cough.. Pertussis.. Infants, Children, high fatal in Adults than children Antibiotics during the first stage  Prevention: Potent single or multiple Vaccine  Diagnosis: Clinical signs and Symptoms. Less Culture & Lab tests.

Neisseria & Moraxella Groups  Neisseria spp., Moraxella spp.: Gram-negative diplococci, Facultative anaerobes, Oxidase/Catalase+ve, Highly susceptible to Low/High Tempt… Dryness, Rapid Autolysis.. Room Tempt. Normal Flora Respiratory Tract.. Rare Non-pathogens (N. sicca, N. flava, M. Mucosa).. Common Pathogens.. N. gonorrhea, N. meningitidites.  1-N.gonorrhea: Pili, IgA-Protease, LPS, Colonization Mucosa.. Invasion..Inflamation.. Genitourinary Tract, Rectum,Throat, Sexually Transmitted Diseases.. Acute/Subacute/Asymptomatic Infections.. Uretral/Vaginal Discharge, Urethritis, Cervicitis, Salpengitis.. Common Reinfection..

Neisseria Gramstain-Intracellular Presence in Urethral Discharge

Follow/2  Lab diagnosis: Direct Gram-stain.. Intracellular G-ve diplococci Culture in Blood/chocolate Agar.. Antibiotics, No Vaccine.  2- N. meningitidites: Capsular Polysaccharides, Serotypes A,B,C, Invasive, Exogenous Infect. Respt.Tract.. Sore Throat.. Septicemia.. Meningitis.. high Mortality.. Epidemic infect.. Children..6-Months-5 years Rare Adults.  Lab Diagnosis: Direct-Gran-stain, Culture in Blood/chocolate Agar.. Antibiotics.. Protective Vaccine.  3- M. catarrhalis: Part of normal Respiratory tract.. Opportunstic pathogens.. Pneumonia.. Rare Septicemia.. Compromised Lung.. Culture.. Antibiotics.