Hybrid systems with nanocellulose: Magnetic responsive nanocellulose for separation technology applications - Protein separation Puu-0.4210 – Nanotechnology.

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Presentation transcript:

Hybrid systems with nanocellulose: Magnetic responsive nanocellulose for separation technology applications - Protein separation Puu – Nanotechnology in Forest Biomaterials Nanna Koivula Advisor: Ilari Filpponen

Introduction Magnetic nanoparticles used in protein separation -> medical applications, affinity filtration etc. (Moreno-Guzmán et al. 2010)

Papain recovery Papain recovery- CNC/Fe 3 O 4 NPs/AuNPs Magnetic core: magnetite nanoparticles (Fe 3 O 4 NPs) Metal or metal oxide shell: Au nanoparticles (AuNPs) (Mahmoud et al. 2013)

Approach Materials TEMPO-ox. cellulose nanocrystals (CNC) Magnetic nanoparticle (Fe 3 O 4 ) Methods Covalent attachment of magnetic nanoparticles to the TEMPO-CNC CNC modification to provide specific affinity towards target protein (addition of Cu2+ forms a ligand which is specific for lysozyme binding; (Zhu, J.; Sun, G (2014)) Fluorescence labelling of protein (for demo purposes) Application Protein isolated from sample solution under magnetic field

References Mahmoud, K. Lam, E. Hrapovic, S. Luong, J Preparation of Well-Dispersed Gold/Magnetite Nanoparticles Embedded on Cellulose Nanocrystals for Efficient Immobilization of Papain Enzyme. ACS applied materials & interfaces.5:11. pg. 4978–4985 Moreno-Guzman, M. Eguılaz, M et al Disposable immunosensor for cortisol using functionalized magnetic particles. Analyst. 135, pg Nacev, A. Beni, C. Bruno, O. Shapiro, B Magnetic nanoparticle transport within flowing blood and into surrounding tissue. 5:11. pg Nypelo, T. Rodriguez-Abreu, C. Rivas, J. Dickey, M, Rojas. O Magneto-responsive hybrid materials based on cellulose nanocrystals. Cellulose. 21. pg. 2557–2566 Peng, J. Liu, Q. Xu, Z. Masliyah, J Synthesis of Interfacially Active and Magnetically Responsive Nanoparticles for Multiphase Separation Applications. Advanced functional materials. 22:8. pg Zhu, J.; Sun, G Facile Fabrication of Hydrophilic Nanofibrous Membranes with an Immobilized Metal–chelate Affinity Complex for Selective Protein Separation. ACS applied materials & interfaces, 6, pg