MS3557 Phosphorylation sites identification of eEF1A in yeast Biological Mass Spectrometry Facility.

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MS3557 Phosphorylation sites identification of eEF1A in yeast Biological Mass Spectrometry Facility

Samples and Methods Samples: eEF1A purified from yeast, 20ug/gel piece X5 20 ug digest with trypsin LC-MSMS: 0.4ug on column, 3hour run. IMAC for phospho-peptide enrichment for the rest of the samples. The phospho-peptides were analyzed by LC-MSMS. 50% on column, 80min run. Database search: GPM

21 STTTGHLIY p-me K meythyl 30

155 MDS p VKWDESR 164 B4-98 B3-98 B5-98 B8-98 NL-98 y3

289 S p VEMHHEQLEQGVPGDNVGFNVK 311 NL-98 y10 b13 2+ b12 2+ b11 2+ b10 2+ b19 2+ b20 2+ b22 2+ b13 y5 y3

312 VNS p VKEIR 319 NL-98 b6 b2 y2 y6 b7 b6-98 y4 b3-98

391 FLKS p GDAALVL 401 B2 NL-98 B3 B6 B8/y9-98 B9 B10 y3 y4 y7 y8 y9 y9 2+ y8-98 B9-98 B6-98 B5-98

402 FVPSK me PMCVEAFSEYPPLGR 421 y2 y18 3+ y18 2+ y5 y4 b15 2+ b b8 b10 2+ b15 S414p/Y416p b2