Formation of a nucleoprotein complex containing Tn7 and its target DNA regulates transposition initiation by Zachary Skelding, Robert Sarnovsky, and Nancy.

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Formation of a nucleoprotein complex containing Tn7 and its target DNA regulates transposition initiation by Zachary Skelding, Robert Sarnovsky, and Nancy L. Craig EMBO J. Volume 21(13): July 1, 2002 ©2002 by European Molecular Biology Organization

(A) Schematic of Tn7 higher ‐ order nucleoprotein complexes. Zachary Skelding et al. EMBO J. 2002;21: ©2002 by European Molecular Biology Organization

(A) The TnsABC+D (L,R)–Tgt complex contains target DNA and requires a donor DNA with two Tn7 ends. Zachary Skelding et al. EMBO J. 2002;21: ©2002 by European Molecular Biology Organization

The TnsABC+D (L,R)–Tgt complex is competent for transposition. Zachary Skelding et al. EMBO J. 2002;21: ©2002 by European Molecular Biology Organization

Mutagenesis of the C ‐ terminus of TnsB strongly decreases transposition and interaction with the target DNA. (A) Alignment of the amino acid sequences from the C ‐ terminus of Tn7 TnsB with Tn5468 TnsB. Zachary Skelding et al. EMBO J. 2002;21: ©2002 by European Molecular Biology Organization

TnsB and TnsCA225V can mediate formation of a (L,R)–Tgt complex, and this reaction depends on residues within the C ‐ terminus of TnsB. Zachary Skelding et al. EMBO J. 2002;21: ©2002 by European Molecular Biology Organization

The TnsABC+D (L,R)–Tgt complex is more stable than the TnsBC+D (L,R)–Tgt complex. Zachary Skelding et al. EMBO J. 2002;21: ©2002 by European Molecular Biology Organization

Mutation of the C ‐ terminus of TnsB does not significantly affect TnsC ‐ stimulated TnsAB cleavage. Zachary Skelding et al. EMBO J. 2002;21: ©2002 by European Molecular Biology Organization