GAS CHROMATOGRAPHY Mr. Maywan Hariono

 One of chromatography technique that use a gas as a carrier (mobile phase)  The separation is based on the volatility of sample  Same with HPLC, GC is one of advance chromatography method and use Rt as a separation parameter

Phase??  Stationary phase: octadecyl silane, octasilane, aminosilane  Mobile phase (gas) : He, N 2, H 2, Ar

The principle and instrumentation of GC

Principles  The volatile sample in the liquid matter is injected to the column.  The temperature of column is setted in which sample can be vaporized.  The gas is pumped and then it carries the sample (gas) through the column.  The sample which has higher boiling point/melting point will be retained by column, in contrast the lower one will be carried through the column faster.  Means the higher b.p/m.p wil be lower the Rt and vice versa

Principles  The information from GC machine will be translated to the graph by software

Komponen2 GC 1. syringe 2. Injection port 3. column 4. GC oven 5. detector 6. Carrier gas 7. recorder

GC Component a. Syringe 5-10 microliter in volume

GC Component b. Injection port

GC Component c. Column

Factors influence the GC separation performance i.Boiling point/melting point ii. Column temperature iii. Column length iv. The speed of gas stream v. Internal diameter of column

GC APPLICATIONS 1. Pharmaceutical industry 2. Herbal medicine industry 3. Environmental analysis 4. Cosmetics analysis

adavntages i. Precise and accurate ii. Sensitive

Disadvantages GC i. Time consuming ii. Only volatile sample can be determined using GC iii. The sample is damaged after running, can not be recollected.

Rujukan 1.David G Watson(2005). Pharmaceutical Analysis. Second edition. Pp: as-chromatography.html 3. support/GC.html