Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Human Research Program (HRP) HRP Increment 37/38 Overview Increment Manager Susan Torney/ NASA Increment Lead Gina Miller/ LM Operations Lead Andres Ovalles/ LM Export Classification: EAR99

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles HRP Increment 37/38 Team NASA HRP Increment Manager Susan Torney Increment Lead Gina Miller Project Scientist Clarence Sams Increment Planner Joe Coen Increment Operations Lead Andres Ovalles Increment Operations Tech Lead Elizabeth Rowan Increment Training Coordinator Sondra Fabian Increment Science Coordinator Matthew Roper (36S crew) Shanna Rodgers (37S crew)

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles AGENDA HRP Inc 37/38 Complement Crew Participation New HRP In-flight Experiments Ocular Health Update Facility Activities Support to IP Activities Forward Work

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles HRP Inc. 37/38 Complement In-flight and Pre/Post  Biochem Profile  Bisphosphonates Control  Body Measures  Cardio Ox  Journals  Microbiome  Ocular Health  Pro K  Reaction Self Test  Repository  Salivary Markers  Spinal Ultrasound  Sprint Pre/Post Only  Functional Task Test  Hip QCT  IVD  Manual Control

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles 35S ESA FE35S NASA FE36S NASA FE 37S JAXA CDR37S NASA FE Bisphosphonates Ctrl  Body Measures 3 Sessions FD15, 80, R-30 2 Sessions FD15, 80 Cardio Ox 2 Sessions FD15, 60 Journals  Microbiome 2 Sessions R-14, R-1 2 Sessions R-14, R-1 4 Sessions FD7, 90, R-14, R-1 2 Sessions FD7,90 Ocular Health 2 Sessions FD120, R-30 2 Sessions FD120, R-30 6 Sessions FD10,30,60,90, 120, R-30 5 Sessions FD10,30, 60, 90, 120 Pro K / Biochem Profile/ Repository 2 Sessions FD120, R-14 (ProK/Rep) 2 Sessions FD120, R-14 (ProK/Rep) 5 Sessions FD15,30,60,120, R-14 (ProK/Biochem/Repo) 4 Sessions FD15,30,60, 120 (Biochem/Repo) Reaction Self Test  Salivary Markers 3 Sessions FD10, 90, R-1 Spinal Ultrasound 1 Session FD Session FD Sessions FD30, 90, Sessions FD30, 90 Sprint 4 VO2 Sessions FD15, 45, 75, USND Sessions FD14,30,60,90, 120 Participation Matrix for HRP Experiments (In-flight)

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles HRP Inc 37/38 New In-flight Experiments Body Measures Cardio Ox Biochem Profile Salivary Markers Sprint* * Sprint is not a new HRP experiment but was not performed in Inc 35/36.

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Body Measures: Sudhakar Rajulu, Ph.D. Brief Research Summary: Currently, NASA does not have sufficient in- flight anthropometric data (body measurements) gathered to assess the impact of physical body shape and size changes on suit sizing. This study will involve collecting anthropometric data (body measurements) using digital still and video imagery and a tape measure to measure segmental length, height, depth, and circumference data for all body segments (i.e., chest, waist, hip, arms, legs, etc.) from astronauts for pre-, post-, and in-flight conditions. In-Flight Data Collection: Circumference measurements with a tape measure along with photographic and video imagery. Investigation Summaries

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Body Measures ~ FD15, 80, R-30 sessions Operations: –Donning body markers & obtaining photographs –Collecting circumference measurements (recorded in DCT) –Collecting videos of Neutral Body Posture –SLAMMD scheduled within +/- 30 days of each nominal session (efficiencies with monthly SLAMMD runs) HW on 52P

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Body Measures Activities will be performed in LAB with D3S cameras setup at CEVIS rack location –CEVIS will be temp stowed during operations Operator will be required: –Setting up tape for calibration activity –Placing body markers on subject –Taking photos –Performing circumference measurements –Neutral body posture (all video is private)  Camera setup  Voice commands –Data transfer (using SSC)

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Cardio Ox: Steven H. Platts, Ph.D. Brief Research Summary: Future human space travel missions may increase the risk of oxidative and inflammatory damage primarily from radiation, but also from psychological stress, reduced physical activity, diminished nutritional standards and exposure to altered oxygen levels during extravehicular activity. There is evidence that higher levels of oxidative and inflammatory stress and associated damage to blood vessels contribute to cardiovascular disease. The purpose of this study is to measure levels of biomarkers in blood and urine that are affected by oxidative and inflammatory stress before, during, and after long duration spaceflight and relate them to the risk of developing atherosclerosis. In-Flight Data Collection: Ultrasound scans (carotid/brachial) with ECG recording, blood draw and 24-h Urine collection. Investigation Summaries

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Cardio Ox Operations –FD 15, 60, R-15/180 Sessions –Three 24 hr Void-by-void urine collections –Three fasting blood draws –Will be scheduled with ProK sessions for operational efficiencies –Three Ultrasound scans (+/-3 days of blood draws) FD15 FD60 R-15/FD180 Ultrasound Session Frozen Blood/Urine Session

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Biochem Profile: Robert Pietrzyk, M.S. Brief Research Summary: Blood and urine are commonly used to assess an astronaut's health as well as conduct research in physiological disciplines by measuring key biomarkers found in these fluids. In support of research studies, this project will collect, process and store blood and urine samples obtained during the preflight, in- flight and postflight phases of ISS missions and maintain a database of results from the analysis of these samples. This database will offer supporting evidence to scientists by providing metabolic profiles of the effects of spaceflight on human physiology. In-Flight Data Collection: 24-hour urine collection, blood draw and subsequent processing. Investigation Summaries

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Biochem Profile Operations –FD 15, 30, 60, 120, 180 sessions –Will be scheduled with Pro K sessions for operational efficiencies –Five 24 hr Void-by-void urine collections –Five fasting blood draws (immediately following urine collection) –SLAMMD once per month FD15FD30 FD60FD120 FD hour urine collection Frozen Blood collection

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Investigation Summaries Salivary Markers: Richard J. Simpson, Ph.D. Brief Research Summary: The Salivary Markers investigation involves the collection of blood, saliva, urine and a health assessment on six subjects pre-, in- and post-flight to determine if spaceflight induced immune system dysregulation increases infection susceptibility or poses a significant health risk to crewmembers onboard the International Space Station. The investigation utilizes a longitudinal, repeated measures design to determine the effects of long-term exposure to microgravity on a host of salivary antimicrobial proteins (AMPs), latent viral reactivation, antibacterial properties of saliva, and blood markers associated with innate host immune defense. In-Flight Data Collection: Blood draw, Saliva sampling, 24-hour urine collection, and Health Assessment using Med Ops’ Data Collection Tool (DCT) FD 90 and R-1 blood samples will return ambient on Soyuz

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Salivary Markers FD-10FD-90* Soyuz Launch Soyuz Landing Handover Soyuz Undock R-1 7 Day Saliva Collection 24 Hour Urine Collection Health Assessment Ambient Blood Collection *The FD-90 window will be flexible to permit ambient blood to be returned on Soyuz. Frozen Blood Collection Operations –FD10, 90*, R-1 –Operational efficiencies when scheduled with other experiments –Saliva collection extends over a 7 day period –Urine collection extends over a 24 hr period –Blood collection and health assessments are performed each session (blood collected and frozen on FD 10, collected ambient on FD 90 and R-1). HW on 52P

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Salivary Markers  Saliva will be collected every day for 7 days (21 samples per FD session)  The daily collection will include one timed sample and two untimed samples.  The timed sample will be collected prior to the untimed samples.  Collections must occur at the beginning of crew day before eating, drinking, or brushing teeth.  Samples must be performed one at a time

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Ocular Health Update Experiment sessions FD10, 30, 60, 90, 120, R-30 –MRID sessions FD30 & R-30 (FD 100 vision testing only per crew surgeon request) Operations –Vision Testing –Tonometry (with blood pressure) –Fundoscopy –Ocular Ultrasound –Cardiac Ultrasound (with blood pressure) Experiment uses current SODF procedures and activity models for most activities –POIC currently schedules Cardiac Ultrasound and blood pressure readings (OH team schedules other activities via BME) Scheduling challenges are expected during Increment 37 with three concurrent subjects on orbit and the complexity of exercise requirements The strategy in Inc 35/36 of designating weeks in the OOS for Ocular Health seemed to work well in highlighting the need for increased coordination between POIC, MedOps & HRF.

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles HRP Inc 37/38 Facility Activities  GDS/PFS Gauge Photos  HRF Supply Kit Inventory/Resupply  SLAMMD Control Run  Ultrasound 2 File Maintenance and Backup  Build 13 DVD Install / RIC 9.0 Install  HRF1 8PU RFID Drawer Config* *Potential carry over from 35/36

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles HRP Support to IP Science Biological Rhythms 48 hr (JAXA) – Uses Actiwatch Reader/Cable BP Reg (CSA) – Uses HRF Continuous Blood Pressure Device (CBPD) & Pulmonary Function System Circadian Rhythms (ESA) – Uses Actiwatch/Cable Energy (ESA) – Uses pulmonary Function System Vascular (CSA) – Uses HRF Centrifuge (RC)

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Inc. 37/38 Open Work New experiment ops products –ECR Body Measures  In review –ECR Cardio Ox / Salivary Markers / Biochem Profile  Products submitted to POIC for ECR submittal  Cardio Ox Safety Data Package to be approved Aug 9 th 2013 Returning experiment ops products –Sprint  Increment 38  Procedures and Planning Data Set updates required –GJOP & removing VO2max callouts  Will be submitted via OCR Operations Efficiencies with Blood & Urine experiments

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Back Up Slides

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Acronyms, Abbreviations, Definitions ASCRSAstronaut Strength, Conditioning, and Rehabilitation Specialist CDRCommander CMDCommand C/OCheck out CSACanadian Space Agency DVDDigital Video Disc ESAEuropean Space Agency FTTFunctional Task Test GDSGas Delivery System HRFHuman Research Facility HRPHuman Research Program IPInternational Partner ISSInternational Space Station ISSMPISS Medical Project (implementation leg of HRP) LMLockheed Martin NASANational Aeronautical Space Agency PFSPulmonary Function System PIGPressurized Inhalation Gases RCRefrigerated Centrifuge RICRack Interface Controller SLAMMDSpace Linear Acceleration Mass Measurement Device SSCShared Station Computer SWSoftware T61ptype of Portable Computer TBDTo Be Determined UMSUrine Monitor System URCUser Requirements Collection WHCWaste Hygiene Compartment

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Intervertebral Disc Damage: Alan Hargens, Ph.D. Brief Research Summary: The goal of this study is to use state-of- the-art imaging technologies to comprehensibly characterize and quantify space-flight induced changes in disc morphology, biochemistry, metabolism, and kinematics. Subjects will be imaged before and after prolonged spaceflight. These data will be correlated with low back pain that spontaneously arises in space so as to establish pain and disc damage mechanisms that will serve as a basis for future countermeasure development. Data Collection: Pre-/Post-flight testing only; involves MRI testing. Pre-/Post- Investigation Summaries

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Pre-/Post- Investigation Summaries Functional Task Test: Stuart M.C. Lee & Barry A. Spiering Brief Research Summary: The primary objective of the Functional Task Test (FTT) project is to develop and evaluate an integrated set of functional and physiological tests, and then use those tests to determine how post flight changes in sensorimotor, cardiovascular, and muscle physiology impact functional performance (e.g. standing, ladder climbing, and hatch opening). Data Collection: Pre-/Post-flight testing only; series of timed muscle performance tests

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Hip QCT: Jean Sibonga, Ph.D. & Thomas Lang, Ph.D. Brief Research Summary: The primary objective of Hip QCT is to monitor changes in hip sub-regions in response to in-flight countermeasures (CM). This QCT study will also demonstrate how countermeasures that involve mechanical loading of the hip (e.g., exercise) could be distinguished from CMs that involve biochemical suppression of bone resorption (e.g., bisphosphonates) because these two categories of CMs affect different bone compartments of the hip (anti-resorptives on trabecular bone, exercise on cortical bone). Finally, QCT will enable hip strength estimations by Finite Element [FE] Modeling – which detects more changes in hip strength due to space than with DXA modality. Data Collection: Pre-/Post-flight testing only; involves QCT assessment. Pre-/Post- Investigation Summaries

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Manual Control: Stephen Moore, Ph.D. Brief Research Summary: Lack of gravity causes sensorimotor deficits post-landing. This experiment's comprehensive cognitive/sensorimotor test battery will determine the relative contribution of specific mechanisms (including sleepiness and fatigue) underlying decrements in post-flight operator proficiency. These results will be critical in determining whether sensorimotor countermeasures are required for piloted landings and early surface operations, and what functional areas countermeasures should target. Data Collection: Pre-/Post-flight testing only; involves physiological and performance measures. Pre-/Post- Investigation Summaries

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Investigation Summaries Spinal Ultrasound : Scott A. Dulchavsky, Ph.D. Brief Research Summary: Sonographic Astronaut Vertebral Examination (Spinal Ultrasound) aims to use ground and space- based studies to fully characterize spinal changes during and after spaceflight. Ground based pre- and post-flight MRI and high fidelity ultrasound, combined with in-flight ultrasound will be used to characterize and assign a mission health risk to microgravity- associated spinal alterations for back pain and potential injury. This research will determine the accuracy of MRI and musculoskeletal ultrasound in characterizing the anatomy/composition of the vertebral unit and develop training methodologies. In-Flight Data Collection: Cervical and Lumbar Ultrasounds

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Investigation Summaries ProK: Scott M. Smith, PhD. Brief Research Summary: The Dietary Intake Can Predict and Protect Against Changes in Bone Metabolism during Spaceflight and Recovery investigation is NASAs first evaluation of a dietary countermeasure to lessen bone loss of astronauts. Pro K proposes that a flight diet with a decreased ratio of animal protein to potassium will lead to decreased loss of bone mineral. In-Flight Data Collection: Controlled/monitored diet with urine and blood samples.

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Investigation Summaries Reaction Self Test: David F. Dinges, Ph.D. Brief Research Summary: The Psychomotor Vigilance Self Test on the International Space Station is a portable 5-minute reaction time task that will allow the crewmembers to monitor the daily effects of fatigue on performance while on board the International Space Station. In-Flight Data Collection: Testing using reaction feedback software, scheduled on specific days and sleep shifted schedules, and around EVAs.

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Bisphosphonates (Control): Adrian Leblanc, Ph.D. & Toshio Matsumoto, Ph.D. Brief Research Summary: Bisphosphonates as a Countermeasure to Space Flight Induced Bone Loss. The purpose of the Bisphosphonates study is to determine whether an antiresorptive agent, in conjunction with the routine in-flight exercise program, protects International Space Station (ISS) crewmembers from the regional decreases in bone mineral density documented on previous ISS missions. Control subjects will not ingest the bisphosphonate pill in order to provide a comparison. In-Flight Data Collection: 24-h Urine collection, Diet/Exercise Logs Investigation Summaries

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Investigation Summaries Ocular Health: Christian Otto, M.D. Brief Research Summary: The International Space Station (ISS) Ocular Health Protocol aims to systematically gather physiological data to characterize the Risk of Microgravity-Induced Visual Impairment/Intracranial Pressure on crewmembers assigned to a 6 month ISS increment. The data collected will mirror Medical Requirements Integration Documents (MRID) requirements and testing performed during annual medical exams with an increase in the frequency of in-flight and post flight testing to more accurately assess changes that occur in the visual, vascular, and central nervous systems upon exposure to microgravity and the resulting fluid shifts. Monitoring in-flight changes, in addition to post flight recovery, is the main focus of this protocol. In-Flight Data Collection: Fundoscopy, Tonometry, Visual Testing, Ocular Ultrasound, BP and Vascular Compliance (cardiac ultrasound, BP, EKG)

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Investigation Summaries Microbiome: Hernan Lorenzi, M.D. Brief Research Summary: The Microbiome experiment investigates changes to astronauts’ immune systems and microbiomes (the collection of microbes that live in and on the human body). These changes can be detected by taking periodic samples from different parts of the body and the surrounding International Space Station (ISS) environment. As part of this study, the likelihood and consequences of alterations in the microbiome due to extreme environments, and the related human health risk, will be assessed. In-Flight Data Collection: Blood, Saliva, Perspiration, Potable water collections; Microbiome (body swab), ISS Surface, and optional Gastrointestinal sampling.

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Journals: Jack Stuster, Ph.D. Brief Research Summary: This study converts behavioral and human factors information contained in confidential journal entries into quantitative data concerning the importance of the various behavioral issues involved in extended-duration space exploration. In-Flight Data Collection: Journal entries from crew using SSCs, first performance creates a file. Data downlinked monthly via OCA.

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles Sprint: Lori Ploutz-Snyder, Ph.D. Brief Research Summary: The purpose of the Sprint study is to evaluate the efficacy of a new integrated resistance and aerobic training program designed to minimize the loss of muscle, bone, and cardiovascular function during ISS missions. In-Flight Data Collection: Exercise perspricption, Sprint Vo2max sessions, USND leg scans.

Payload Operations Integration Working Group (POIWG) July 2013 Human Research Program (HRP) Andres Ovalles RFID Benefits –Reduces crew time  Near-simultaneous read of hundreds of tags compared with 1-at-a-time with optical bar code.  Does not require line-of-sight associated with optical bar code approach.  Less crew time needed to perform inventories/ lost item searches. –Reduces stowage volume  RFID for consumables tracking to optimize in-flight inventory and resupply strategies.  Wiser use of 8 PU drawer, instead of 4 PU Drawer