Albumin MIP Prepared in Aqueous Solution System Speaker : Thong-Yueh Lin 報告者 : 林 聰 樂

Outline Introduction Experimental Results and discussion Conclusions

Introduction

Property of Albumin Half-life: 17 to 23 day Molecular weight: 68,000 Isoelectric point: 4.8 Concentration of human serum: 3.0 to 5.0 g/dl

Experimental

Materials Monomer 3-dimethylaminopropyl metacrlamide:DMAPMA acrylamide:AMAllylamine:AA Methacrylic acid:MAA Hydroxy ethyl methacrylate:HEMA

Crosslinking agent Tetraethylene glycol dimethacrylate:TEGDMA Polyethylene glycol dimethacrylate:PEGDMA Materials

Initiators Ammonium persulfate:APS Cumene hydroperoxide:CHP Tetramethyl ethylenediamine:TMEDA Materials

Fabrication Sequence of MIP Preparation of MIPcrush of MIP Screening of MIP Washing with 20 wt.% methanol and drying of MIP

Results and discussion

HPLC analysis of protein mixture

Typical calibration curve of Cytochrome C by using HPLC

Typical calibration curve of Albumin by using HPLC

Typical calibration curve of Lysozyme by using HPLC

Typical calibration curve of Myoglobin by using HPLC

Selectivity mass ratio to adsorbent of M.I.P Sample name: DMAPMA-TEGDMA Albumin 4.5% Water 10.4% DMAPMA 5.0% TEGDMA 80% APS,TMEDA Selectivity: 1:1.9:14.2:1. 4

Selectivity mass ratio to adsorbent of M.I.P Sample name: MAA-TEGDMA Albumin 3.1% Water 7.3% MAA 2.7% TEGDMA 86.8% APS,TMEDA Selectivity: 52.2:160.1: 1:77.5

Selectivity mass ratio to adsorbent of M.I.P Sample name: AM-TEGDMA 1 Albumin 2.4% Water 9.2% AM 2.4% TEGDMA 86% APS,TMEDA Selectivity: 1:4.1:6.5:2.5

Selectivity mass ratio to adsorbent of M.I.P Sample name: AM-TEGDMA 2 Albumin 2.4% Water 11.2% AM 2.3% TEGDMA 84% CHP,TMEDA Selectivity 4.4:25.8:8.9:1

Selectivity mass ratio to adsorbent of M.I.P Sample name: HEMA-PEGDMA Albumin 4.5% Water 10.5% HEMA 5.1% PEGDMA 80% APS,TMEDA Selectivity 1: 4.3: 1: 1.8

Selectivity mass ratio to adsorbent of M.I.P Sample name: DMAPMA-PEGDMA Albumin 5.4% Water 12.6% DMAPMA 6.0% PEGDMA 76% APS,TMEDA Selectivity 3.1:6.4:5.4:1

Mass ratio of Adsorbent for different composition MIP Sample name Mass ratio of adsorbent (Cytochrome C: Lysozyme: Albumin: Myoglobin) DMAPMA-TEGDMA 1 : 1.9 : 14.2 : 1.4 MAA-TEGDMA 52.2 : : 1 : 77.5 AM-TEGDMA 1 : 4.1 : 6.5 : 2.5 AM-TEGDMA 4.4 : 25.8 : 8.9 : 1 HEMA-PEGDMA 1 : 4.3 : 1 : 1.8 DMAPMA-PEGDMA 3.1 : 6.4 : 5.4 : 1

Conclusions 1.The DMAPMA-TEGDMA MIP can obtain high selectivity for albumin selectivity for albumin 2.The mass ratio of DMAPMA-TEGDMA MIP for adsorbent = Albumin : Lysozyme : Myoglobin : Cytochrome C = Albumin : Lysozyme : Myoglobin : Cytochrome C = 14.2 : 1.9 : 1.4 : 1 = 14.2 : 1.9 : 1.4 : 1

Thank you for your attention