miRNA ‐ dependent gene silencing involving Ago2 ‐ mediated cleavage of a circular antisense RNA by Thomas B Hansen, Erik D Wiklund, Jesper B Bramsen, Sune B Villadsen, Aaron L Statham, Susan J Clark, and Jørgen Kjems EMBO J. Volume 30(21): November 2, 2011 ©2011 by European Molecular Biology Organization

miR ‐ 671 represses CDR1 expression. Thomas B Hansen et al. EMBO J. 2011;30: ©2011 by European Molecular Biology Organization

Characterization of the circular CDR1 antisense RNA. (A) Schematic illustration of the circular antisense RNA including the NAS splice donor (SD) and splice acceptor (SA) sites and the position of the NAS ‐ specific primer set. Thomas B Hansen et al. EMBO J. 2011;30: ©2011 by European Molecular Biology Organization

Strand ‐ specific quantification of CDR1 mRNA and the antisense RNA. (A) Schematic illustration of the CDR1 locus with primers used for strand ‐ specific qRT–PCR of CDR1 mRNA (above) and antisense RNA (below), respectively. Thomas B Hansen et al. EMBO J. 2011;30: ©2011 by European Molecular Biology Organization

Effect of CDR1 antisense ‐ specific knockdown and overexpression. Thomas B Hansen et al. EMBO J. 2011;30: ©2011 by European Molecular Biology Organization

Ago2 ‐ mediated cleavage of the CDR1 antisense transcript. Thomas B Hansen et al. EMBO J. 2011;30: ©2011 by European Molecular Biology Organization