CHROMATOGRAPHY 1Biochemistry of Medics
Chromatography 2Biochemistry of Medics
Chromatography 3Biochemistry of Medics
Chromatography- Principle 4Biochemistry of Medics
Chromatography- Classification 5Biochemistry of Medics
Chromatography- Classification(Contd.) 6Biochemistry of Medics
Partition Chromatography 7Biochemistry of Medics
Paper chromatography 8Biochemistry of Medics
Paper chromatography 9Biochemistry of Medics
Paper chromatography- Procedure 10Biochemistry of Medics
Paper chromatography- Procedure 11Biochemistry of Medics
Ascending and Descending Paper Chromatography 12Biochemistry of Medics
Paper Chromatography -Analysis 13Biochemistry of Medics
R ƒ value 14Biochemistry of Medics
Two dimensional chromatography 15Biochemistry of Medics
Significance of Paper chromatography 16Biochemistry of Medics
Thin layer chromatography (TLC) 17Biochemistry of Medics
TLC- Procedure 18Biochemistry of Medics
TLC -Overview 19Biochemistry of Medics
Significance of TLC 20Biochemistry of Medics
Advantages of TLC over paper chromatography 21Biochemistry of Medics
2)Adsorption chromatography 22Biochemistry of Medics
2)Adsorption chromatography- Elution 23Biochemistry of Medics
Adsorption chromatography The substance loosely adsorbed to the stationary medium comes in the earlier fraction. 24Biochemistry of Medics
Ion – Exchange chromatography 25Biochemistry of Medics
Ion – Exchange chromatography 26Biochemistry of Medics
Ion – Exchange chromatography- Procedure 27Biochemistry of Medics
Ion – Exchange chromatography- Procedure (Contd.) 28Biochemistry of Medics
Types of ion exchanges resins 29Biochemistry of Medics
Ion – Exchange chromatography- An Overview 30Biochemistry of Medics
4) Gel filtration chromatography or Molecular Sieve chromatography 31Biochemistry of Medics
Gel filtration chromatography or Molecular Sieve chromatography 32Biochemistry of Medics
Gel filtration chromatography-An Overview Larger particles come out first, while smaller particles come in later fractions 33Biochemistry of Medics
5) Affinity chromatography 34Biochemistry of Medics
Affinity chromatography- Significance 35Biochemistry of Medics
Affinity chromatography- Significance By using antibodies, antigens can be easily separated. Conversely, antibodies can be purified by passing through a column containing the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, the remaining antibodies come out in earlier fractions. 36 Biochemistry of Medics
6) High performance liquid chromatography- 37Biochemistry of Medics
High performance liquid chromatography- 38Biochemistry of Medics
High performance liquid chromatography- Significance 39Biochemistry of Medics
High performance liquid chromatography- Apparatus 40Biochemistry of Medics
6) Gas liquid chromatography- 41Biochemistry of Medics
Gas liquid chromatography- 42Biochemistry of Medics
Gas liquid chromatography-Apparatus 43Biochemistry of Medics
Summary 44Biochemistry of Medics